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Direct observation of the nanoscale dynamics of membrane lipids in a living cell. Nature.
Eggeling, C.; et al.
Nature, 457, 1159-1162 (2009)
Kirill Kolmakov et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 18(41), 12986-12998 (2012-09-13)
Fluorescent dyes emitting red light are frequently used in conventional and super-resolution microscopy of biological samples, although the variety of the useful dyes is limited. We describe the synthesis of rhodamine-based fluorescent dyes with absorption and emission maxima in the...
Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission.
Ge, J.; Kuang, C.; Lee, SS.; Kao, FJ
Optics Express, 20(27), 28216-28216 (2012)
Harpreet Singh et al.
Mitochondrion, 12(2), 230-236 (2011-10-11)
The visualization and quantification of mitochondria-associated proteins with high power microscopy methods is of particular interest to investigate protein architecture in this organelle. We report the usage of a custom-made STimulated Emission Depletion (STED) fluorescence nanoscope with ~30nm lateral resolution...
Analysis of replication factories in human cells by super-resolution light microscopy.
Cseresnyes, Z.; et al.
BMC Cell Biology, 10(1), 88-88 (2009)
STED with wavelengths closer to the emission maximum.
Vicidomini, G.; et al.
Optics Express, 20(5), 5225-5236 (2012)
Marina I Giannotti et al.
Biomacromolecules, 12(7), 2524-2533 (2011-05-25)
Nanopharmaceutics composed of a carrier and a protein have the potential to improve the activity of therapeutical proteins. Therapy for lysosomal diseases is limited by the lack of effective protein delivery systems that allow the controlled release of specific proteins...
A novel nanoscopic tool by combining AFM with STED microscopy.
Harke, B., et al.
Optical Nanoscopy, 1, 3-3 (2012)
STED nanoscopy. A glimpse into the future.
Bianchini, P.; et al.
Cell and Tissue Research, 360(1), 143-150 (2015)
Nasrin Aliasgharlou et al.
Turkish journal of chemistry, 44(6), 1723-1732 (2021-01-26)
In this study, boric acid (BA) is employed as a crosslinking agent to improve the characteristics of two commonly used polymeric films, ethyl cellulose (EC) and polyvinyl alcohol (PVA), for topical drug delivery applications. The developed films are characterized by...
Functional and morphological preservation of adult ventricular myocytes in culture by sub-micromolar cytochalasin D supplement.
Tian, Q.; et al.
Journal of Molecular and Cellular Cardiology, 52(1), 113-124 (2012)
Ultrafast photon counting applied to resonant scanning STED microscopy.
Wu, X.; Toro, L.; Stefani, E.; Wu, Y.
Journal of Microscopy, 257(1), 31-38 (2015)
Fluoreszierende Rhodamine und fluorogene Carbopyronine fur die STED-Mikroskopie lebender Zellen.
Butkevich, Alexey N.; et al.
Angewandte Chemie (International Edition in English), 128(10), 3350-3355 (2016)
Experimental investigations on fluorescence excitation and depletion of ATTO 390 dye.
Luo, D., et al.
Optics & Laser technology, 45, 723-725 (2013)
STED microscopy to monitor agglomeration of silica particles inside A549 cells.
Schubbe, S., et al.
Advanced Engineering Materials, 12, 417-422 (2010)
Snap-, CLIP- and Halo-Tag Labelling of Budding Yeast Cells.
Stagge, F.; et al.
PLoS ONE, 8(10), e78745-e78745 (2013)
Super-Resolution Imaging Reveals That AMPA Receptors Inside Synapses Are Dynamically Organized in Nanodomains Regulated by PSD95.
Nair D.; et al.
The Journal of Neuroscience, 33(32), 13204-13224 (2013)
An Advanced Tool to Reveal Spatiotemporal Heterogeneity of Molecular Membrane Dynamics.
Vicidomini, G.; et al.
Nano Letters, 15(9), 5912-5918 (2015)
STED microscope with Spiral Phase Contrast.
Lauterbach, M. A.; et al.
Scientific Reports, 3 (2013)
Stimulated emission depletion-based raster image correlation spectroscopy reveals biomolecular dynamics in live cells.
Hedde P.N.; et al.
Nature Communications, 4, 2093-2093 (2013)
Two-photon excitation STED microscopy by utilizing transmissive liquid crystal devices.
Otomo, K.; et al.
Optics Express, 22(23), 28215-28215 (2014)
S E D Webb et al.
Optics express, 16(25), 20258-20265 (2008-12-10)
We combine single molecule fluorescence orientation imaging with single-pair fluorescence resonance energy transfer microscopy, using a total internal reflection microscope. We show how angles and FRET efficiencies can be determined for membrane proteins at the single molecule level and provide...
Silvia Zorrilla et al.
Biochemistry, 46(51), 14996-15008 (2007-12-07)
CggR is the transcriptional repressor of the gapA operon encoding central glycolytic enzymes in Bacillus subtilis. Recently, a detailed mechanistic characterization of gapA induction revealed that the binding of fructose-1,6-bisphosphate (FBP) to a low affinity site on CggR (Kd >...
Resolving the structure of inner ear ribbon synapses with STED microscopy.
Rutherford, M.A.
Synapse, 69(5), 242-255 (2015)
Synaptic membrane proteins form stable microdomains in early endosomes.
Geumann, U.; et al.
Microscopy Research and Technique, 73(6), 606-617 (2010)
SNARE Function Is Not Involved in Early Endosome Docking.
Geumann, U.; et al.
Molecular Biology of the Cell, 19(12), 5327-5337 (2008)
Annedore Punge et al.
Microscopy research and technique, 71(9), 644-650 (2008-06-03)
Tackling biological problems often involves the imaging and localization of cellular structures on the nanometer scale. Although optical super-resolution below 100 nm can be readily attained with stimulated emission depletion (STED) and photoswitching microscopy methods, attaining an axial resolution <100...
Enhancing stimulated emission-based fluorescence detection with interferometric setup.
Chung, S.-S.M.; Deng, J.-H.
Proceedings of SPIE, 8948, 89481-89481 (2014)
Super-resolution imaging of ciliary microdomains in isolated olfactory sensory neurons using a custom STED microscope.
Meyer, S. A.; Ozbay, B.
Proceedings of SPIE, 8950, 89500-89500 (2014)
STED Microscopy to Monitor Agglomeration of Silica Particles Inside A549 Cells.
Schubbe, S.; Cavelius, C.
Advanced Energy Materials, 12, 417-422 (2010)
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