Chemistry (Weinheim an der Bergstrasse, Germany), 18(41), 12986-12998 (2012-09-13)
Fluorescent dyes emitting red light are frequently used in conventional and super-resolution microscopy of biological samples, although the variety of the useful dyes is limited. We describe the synthesis of rhodamine-based fluorescent dyes with absorption and emission maxima in the...
Resolving the structure of inner ear ribbon synapses with STED microscopy.
Synapse, 69(5), 242-255 (2015)
STED with wavelengths closer to the emission maximum.
Nanopharmaceutics composed of a carrier and a protein have the potential to improve the activity of therapeutical proteins. Therapy for lysosomal diseases is limited by the lack of effective protein delivery systems that allow the controlled release of specific proteins...
Functional and morphological preservation of adult ventricular myocytes in culture by sub-micromolar cytochalasin D supplement.
Tian, Q.; et al.
Journal of Molecular and Cellular Cardiology, 52(1), 113-124 (2012)
Experimental investigations on fluorescence excitation and depletion of ATTO 390 dye.
Luo, D., et al.
Optics & Laser technology, 45, 723-725 (2013)
Ultrafast photon counting applied to resonant scanning STED microscopy.
Wu, X.; Toro, L.; Stefani, E.; Wu, Y.
Journal of Microscopy, 257(1), 31-38 (2015)
A novel nanoscopic tool by combining AFM with STED microscopy.
Harke, B., et al.
Optical Nanoscopy, 1, 3-3 (2012)
Direct observation of the nanoscale dynamics of membrane lipids in a living cell. Nature.
Eggeling, C.; et al.
Nature, 457, 1159-1162 (2009)
Analysis of replication factories in human cells by super-resolution light microscopy.
The visualization and quantification of mitochondria-associated proteins with high power microscopy methods is of particular interest to investigate protein architecture in this organelle. We report the usage of a custom-made STimulated Emission Depletion (STED) fluorescence nanoscope with ~30nm lateral resolution...
STED microscopy to monitor agglomeration of silica particles inside A549 cells.
CggR is the transcriptional repressor of the gapA operon encoding central glycolytic enzymes in Bacillus subtilis. Recently, a detailed mechanistic characterization of gapA induction revealed that the binding of fructose-1,6-bisphosphate (FBP) to a low affinity site on CggR (Kd >...
We combine single molecule fluorescence orientation imaging with single-pair fluorescence resonance energy transfer microscopy, using a total internal reflection microscope. We show how angles and FRET efficiencies can be determined for membrane proteins at the single molecule level and provide...
Two-photon excitation STED microscopy by utilizing transmissive liquid crystal devices.
Otomo, K.; et al.
Optics Express, 22(23), 28215-28215 (2014)
Synaptic membrane proteins form stable microdomains in early endosomes.
Geumann, U.; et al.
Microscopy Research and Technique, 73(6), 606-617 (2010)
Super-resolution imaging of ciliary microdomains in isolated olfactory sensory neurons using a custom STED microscope.
Meyer, S. A.; Ozbay, B.
Proceedings of SPIE, 8950, 89500-89500 (2014)
STED nanoscopy. A glimpse into the future.
Bianchini, P.; et al.
Cell and Tissue Research, 360(1), 143-150 (2015)
Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission.
Ge, J.; Kuang, C.; Lee, SS.; Kao, FJ
Optics Express, 20(27), 28216-28216 (2012)
Quantification of Internalized Silica Nanoparticles via STED Microscopy.
Peuschel, H.; et al.
BioMed Research International, 2015(1), 1-16 (2015)
STED Nanoscopy in Living Cells Using Fluorogen Activating Proteins.
Fitzpatrick, JA.; et al.
Bioconjugate Chemistry, 20(10), 1843-1847 (2009)
Far-field optical nanoscopy based on continuous wave laser stimulated emission depletion.
Kuang, C.; Zhao, W.; Wang, G.; et al.
The Review of Scientific Instruments, 81(5), 53709-53709 (2010)
Microscopy research and technique, 71(9), 644-650 (2008-06-03)
Tackling biological problems often involves the imaging and localization of cellular structures on the nanometer scale. Although optical super-resolution below 100 nm can be readily attained with stimulated emission depletion (STED) and photoswitching microscopy methods, attaining an axial resolution <100...
Superresolution and Fluorescence Dynamics Evidence Reveal That Intact Liposomes Do Not Cross the Human Skin Barrier.
Nuclear actin-related proteins (Arps) are subunits of several chromatin remodelers, but their molecular functions within these complexes are unclear. We report the crystal structure of the INO80 complex subunit Arp8 in its ATP-bound form. Human Arp8 has several insertions in...
An Advanced Tool to Reveal Spatiotemporal Heterogeneity of Molecular Membrane Dynamics.
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