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Bo-Ram Kim et al.
Tissue engineering. Part A, 20(23-24), 3279-3289 (2014-06-18)
To confirm the effect of recombinant human bone morphogenetic protein-2 (BMP-2) for bone regeneration, BMP-2-loaded polycaprolactone (PCL)-gelatin (Gel)-biphasic calcium phosphate (BCP) fibrous scaffolds were fabricated using the electrospinning method. The electrospinning process to incorporate BCP nanoparticles into the PCL-Gel scaffolds
Thomas S Ream et al.
Nucleic acids research, 43(8), 4163-4178 (2015-03-31)
Using affinity purification and mass spectrometry, we identified the subunits of Arabidopsis thaliana multisubunit RNA polymerases I and III (abbreviated as Pol I and Pol III), the first analysis of their physical compositions in plants. In all eukaryotes examined to
Giorgia Manzo et al.
Biopolymers, 97(11), 873-881 (2012-08-18)
Antimicrobial peptides (AMPs) are found in various classes of organisms as part of the innate immune system. Despite high sequence variability, they share common features such as net positive charge and an amphipathic fold when interacting with biologic membranes. Esculentin-1b
Frank O Aylward et al.
PloS one, 10(8), e0134752-e0134752 (2015-09-01)
Leaf-cutter ants are prolific and conspicuous constituents of Neotropical ecosystems that derive energy from specialized fungus gardens they cultivate using prodigious amounts of foliar biomass. The basidiomycetous cultivar of the ants, Leucoagaricus gongylophorus, produces specialized hyphal swellings called gongylidia that
Silvia Surinova et al.
EMBO molecular medicine, 7(9), 1166-1178 (2015-08-09)
Non-invasive detection of colorectal cancer with blood-based markers is a critical clinical need. Here we describe a phased mass spectrometry-based approach for the discovery, screening, and validation of circulating protein biomarkers with diagnostic value. Initially, we profiled human primary tumor
Bradley Peter et al.
Biochemistry, 52(16), 2739-2749 (2013-04-04)
Chloride intracellular channel protein 1 (CLIC1) is a dual-state protein that can exist either as a soluble monomer or in an integral membrane form. The transmembrane domain (TMD), implicated in membrane penetration and pore formation, comprises helix α1 and strand
Anisur R Molla et al.
Biochimie, 95(2), 204-214 (2012-10-02)
2,2,2-Trifuoroethanol (TFE)-induced conformational structure change of a β-sheet legume lectin, soybean agglutinin (SBA) has been investigated employing its exclusive structural forms in quaternary (tetramer) and tertiary (monomer) states, by far- and near-UV CD, FTIR, fluorescence, low temperature phosphorescence and chemical
Aase M Raddum et al.
Biochemical pharmacology, 95(1), 1-15 (2015-03-17)
The progression of aggressive cancer occurs via angiogenesis and metastasis makes these processes important targets for the development of anti-cancer agents. However, recent studies have raised the concern that selective inhibition of angiogenesis results in a switch towards increased tumour
Claudia Scholz et al.
ChemMedChem, 9(9), 2104-2110 (2014-02-08)
The optimization of synthetic carriers for gene transfer remains a major challenge. Cationic polymers such as polyethylenimine (PEI) often show increasing gene transfer activity with increasing molecular weight, but this favorable effect is accompanied by an undesired increase in cytotoxicity.
Thiloka C Ariyasena et al.
Journal of chromatography. A, 1361, 240-254 (2014-08-30)
Retention factors on several columns and at various temperatures using gas chromatography and from reversed-phase liquid chromatography on a SunFire C18 column with various mobile phase compositions containing acetonitrile, methanol and tetrahydrofuran as strength adjusting solvents are combined with liquid-liquid
Muhammad Abbas et al.
Organic & biomolecular chemistry, 10(47), 9330-9333 (2012-11-02)
Ammonia and selenoaldehydes are both problematic components in Ugi reactions. Here we report the efficient direct multicomponent synthesis of sensitive selenocysteine peptides without the use of convertible (protected) primary amines, including suitable deprotection protocols for selenols.
Akiyoshi Hirata et al.
Journal of the American Chemical Society, 136(32), 11546-11554 (2014-07-19)
A polyamide containing N-methylpyrrole (Py) and N-methylimidazole (Im), designated PIPA, binds with high affinity and specificity to specific nucleotide sequences in the minor groove of double-helical DNA. Based on a recent report of the synthesis of PIPA for telomere visualization
Trifluoroethanol-induced conformational change of tetrameric and monomeric soybean agglutinin: Role of structural organization and implication for protein folding and stability.
Molla AR, Mandal DK.
Biochimie (2012)
Michael Reinwarth et al.
Chembiochem : a European journal of chemical biology, 14(1), 137-146 (2012-12-12)
Bioactive peptides often contain several disulfide bonds that provide the main contribution to conformational rigidity and structural, thermal, or biological stability. Among them, cystine-knot peptides-commonly named "knottins"-make up a subclass with several thousand natural members. Hence, they are considered promising
A facile parallel synthesis of trifluoroethyl-substituted alkynes.
Cui-Bo Liu et al.
Angewandte Chemie (International ed. in English), 51(25), 6227-6230 (2012-05-09)
John R Horsley et al.
Journal of the American Chemical Society, 136(35), 12479-12488 (2014-08-15)
Electrochemical studies are reported on a series of peptides constrained into either a 310-helix (1-6) or β-strand (7-9) conformation, with variable numbers of electron rich alkene containing side chains. Peptides (1 and 2) and (7 and 8) are further constrained
Claudia Kunz et al.
Journal of peptide science : an official publication of the European Peptide Society, 18(6), 400-404 (2012-04-24)
The influence of lithium cations on the cis/trans isomerization of prolyl peptide bonds was investigated in a quantitative manner in trifluoroethanol (TFE) and acetonitrile, employing NMR techniques. The focus was on various environmental and structural aspects, such as lithium cation
Yinyin Chen et al.
Dalton transactions (Cambridge, England : 2003), 44(7), 3118-3127 (2015-01-09)
A composite antitumor drug carrier platform, in which antitumor drug doxorubicin (DOX) loaded core-shell structured Cu9S5@mSiO2 nanoparticles were incorporated into poly(ε-caprolactone) and gelatin to form nanofibrous fabrics using an electrospinning process, was successfully assembled. The resultant multifunctional spun pieces could
Johnny Habchi et al.
Methods in molecular biology (Clifton, N.J.), 895, 361-386 (2012-07-05)
Electron paramagnetic resonance (EPR) spectroscopy is a technique that specifically detects unpaired electrons. EPR sensitive reporter groups (spin labels or spin probes) can be introduced into biological systems via site-directed spin labeling (SDSL). This is usually accomplished by cysteine-substitution mutagenesis
Herbert C Hoffmann et al.
Chemical communications (Cambridge, England), 48(85), 10484-10486 (2012-08-31)
Recently, we have described the synthesis of chiral metal-organic frameworks iPr-ChirUMCM-1 and Bn-ChirUMCM-1 and their use in enantioselective separation. Here, we demonstrate for the first time the use of a chiral solvating agent (1-phenyl-2,2,2-trifluoroethanol, TFPE) for chiral recognition in iPr-ChirUMCM-1
Catherine Pan et al.
Journal of proteome research, 13(11), 4535-4545 (2014-05-24)
Despite extensive research, an unmet need remains for protein biomarkers of Parkinson's disease (PD) in peripheral body fluids, especially blood, which is easily accessible clinically. The discovery of such biomarkers is challenging, however, due to the enormous complexity and huge
Timo Glatter et al.
Molecular systems biology, 5, 237-237 (2009-01-22)
Protein complexes represent major functional units for the execution of biological processes. Systematic affinity purification coupled with mass spectrometry (AP-MS) yielded a wealth of information on the compendium of protein complexes expressed in Saccharomyces cerevisiae. However, global AP-MS analysis of
V L Anderson et al.
Physical biology, 9(5), 056005-056005 (2012-08-31)
Both increased temperature and moderate concentrations of fluorinated alcohols enhance aggregation of the Parkinson's disease-associated protein α-synuclein (αS). Here, we investigate the secondary structural rearrangements induced by heating and trifluoroethanol [TFE]. At low TFE concentrations, CD spectra feature a negative
Neeraj Kumar Joshi et al.
The journal of physical chemistry. A, 116(27), 7272-7278 (2012-06-14)
In the present work we report some hitherto unnoticed features in the steady state and time-resolved measurements of isoquinoline in water and trifluoroethanol (TFE). Absorption spectra reveal that in water, neutrals as well cationic species are present. Emission spectrum shows
Ganesh Shanmugam et al.
International journal of biological macromolecules, 54, 155-159 (2012-12-19)
Collagen, a fibrous structural protein, is a major component of skin, tendon, bone, and other connective tissues. Collagen is one of the dominant biomaterials used for tissue engineering and drug delivery applications. 2,2,2-Trifluoroethanol (TFE) has been used as a co-solvent
Qiang Shao et al.
The Journal of chemical physics, 137(14), 145101-145101 (2012-10-16)
To investigate how solvent (water) and cosolvent (osmolyte) affect protein structure in various osmolyte solutions, in the present study we used GB1p peptide as the model protein to study its folding process in 2,2,2-trifluoroethanol (TFE)∕water and denaturation process in GdmSCN∕water
Emma-Dune Leriche et al.
Molecules (Basel, Switzerland), 19(12), 20731-20750 (2014-12-17)
Highly branched polyamidoamine (PAMAM) dendrimers presenting biological activities have been envisaged as non-viral gene delivery vectors. They are known to associate with nucleic acid (DNA) in non-covalent complexes via electrostatic interactions. Although their transfection efficiency has been proved, PAMAMs present
Ariane E Erickson et al.
Carbohydrate polymers, 134, 467-474 (2015-10-03)
The inability to produce large quantities of nanofibers has been a primary obstacle in advancement and commercialization of electrospinning technologies, especially when aligned nanofibers are desired. Here, we present a high-throughput centrifugal electrospinning (HTP-CES) system capable of producing a large
Bei Feng et al.
Biomacromolecules, 13(12), 3917-3925 (2012-11-08)
In tissue engineering research, there has recently been considerable interest in using electrospun biomimetic nanofibers of hybrids, in particular, from natural and synthetic polymers for engineering different tissues. However, phase separation between a pair of much dissimilar polymers might give
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