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Determination of 7 Nitrosamines in Drinking Water

Nitrosamines analysis by solid phase extraction (SPE) and subsequent gas chromatography-tandem mass spectrometry (GC-MS/MS) according to GB 5750.10-2023.

MilliporeSigma Announces € 62 Million Investment in New Quality Control Facility in Darmstadt, Germany

Facility to provide production-related quality control services for Life Science business and brings together 135 employees and various functions into one ~10,000 m2 building.

Estimation of Proline in Fruit and Vegetable Juices

Spectrophotometric determination of proline by the Ninhydrin method, per EN 1141 and German Food and Feed Code §64 LFBG 31.00-7.

RNA Therapeutics & Delivery USA

Visit us at booth #7 to learn about our solutions to help you overcome your mRNA manufacturing and LNP formulation challenges.

cOmplete™, Mini, EDTA-free Protocol

Dissolve one tablet in 10 ml aqueous buffer or water. If high proteolytic activity is present, dissolve one tablet in 7 ml buffer.

Scepter™ 3.0 Cell Counter – How it Works

Scepter™ counting is 7 to 10 times faster than hemocytometry-and also faster than other automated counters. Learn more about how this amazing technology works

Enzymatic Assay of Cathepsin B

Cathepsin B is a lysosomal cysteine proteinase with broad specificity. This protocol uses Nα–CBZ–Arg–Arg–7–amido–4–methylcoumarin as the substrate for fluorometric detection of Cathepsin B activity.

Assay of Betamethasone Sodium Phosphate by HPTLC

Development of an HPTLC based method, using Silica Gel 60 F254 plate, to determine Betamethasone Sodium Phosphate over a concentration range of 1 to 7 µg/spot.

Ultrapure Water for Sensitive LC-MS Analysis of Pesticides

Pesticides analysis by LC-MS needs pesticide-free reagent water, which can be reliably delivered by Milli-Q® IQ 7 ultrapure water systems. See data.

In Vitro Differentiation of Human PBMC and CD34+ Derived Monocytes into Mature CD83+/CD14- Dendritic Cells

Generate mature human dendritic immune cells from PBMCs or CD34+ stem cell derived monocytes in 7 days in defined and serum-free dendritic cell culture media. Explore over 350 PromoCell products.

Obtaining Ultrapure Water for Sensitive PFAS Analysis by LC-MS

Sensitive PFAS testing can be performed with ultrapure water from a Milli-Q® IQ 7 system, which eliminates detectable PFAS for reliable LC-MS/MS results. See the science, see the proof.

3D Mammosphere Culture Media

3D mammosphere culture protocol used to expand human MCF-7 and MDA-MB-231 breast cancer cells in serum-free/xeno-free stem cell culture media. Explore over 350 PromoCell products on Sigma.com.

Carreira DOLEFIN Ligands

The selective preparation of diarylmethine stereogenic centers is a challenging endeavor in chemical synthesis, especially when there is little differentiation between the two aryl groups

Restriction Endonucleases - The Molecular Scissors

The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson.

TRANSIA® PLATE Staphylococcal Enterotoxins

TRANSIA® PLATE Staphylococcal Enterotoxins - AOAC Performance Tested Methods Validation Study

NHC-based Palladium Catalysts

In collaboration with Umicore AG and Co.,1 we are pleased to offer a series of robust Pd(II) and Pd(0) complexes employed as the linchpin in C–C bond forming reactions.

Metal Borohydrides as Hydrogen Storage Materials

An article about metal borohydrides as hydrogen storage materials

Organic Semiconductor Laser Materials

Poly(Amino Acid) Block Copolymers for Drug Delivery and other Biomedical Applications

Humankind has utilized protein materials throughout its existence, starting with the use of materials such as wool and silk for warmth and protection from the elements and continuing with the use of recombinant DNA techniques to synthesize proteins with unique

Cell Death Troubleshooting in Cell Culture

Here we discuss causes of cell death, and how to assess and prevent these causes.

Optimizing Crude Sample Plant PCR

Overcoming potent inhibitors and sample diversity in direct plant PCR.

Recent Developments in Magnetic Iron Oxide Nanoparticles for Non-Invasive Bioimaging

Professor Hui Mao explores the use of superparamagnetic iron oxide nanoparticles (INOPs) that offer an alternate contrast-enhancing mechanism.

Quantum Dots: An Emerging Class of Soluble Optical Nanomaterials

We presents an article concering Quantum Dots: An Emerging Class of Soluble Optical Nanomaterials.

Complete Whole Transcriptome Amplification Kit Protocol (WTA2)

WTA2, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3-bias

Biginelli Reaction

The Biginelli Reaction is an acid-catalyzed, three-component reaction between an aldehyde, b-ketoester, and urea that produces tetrahydropyrimidones, which have potential pharmaceutical applications.

Determination of 2-Chloroethanol as Marker for Fumigant Ethylene Oxide in Sesame Seeds by HS-SPME-GC-MS

The work describes the development of an HS-SPME-GC-MS method to determine 2-chloroethanol as a marker of fumigant ethylene oxide in different kinds of sesame seed samples.

Immunoprecipitation Techniques

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

Purification Using Protein A-based Chromatography Media

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

Degradable Poly(ethylene glycol) Hydrogels for 2D and 3D Cell Culture

Progress in biotechnology fields such as tissue engineering and drug delivery is accompanied by an increasing demand for diverse functional biomaterials. One class of biomaterials that has been the subject of intense research interest is hydrogels, because they closely mimic

Thermal Transitions of Homopolymers: Glass Transition & Melting Point

Literature values for the glass transition temperature, (Tg), and melting temperature, (Tm), are given for the more common homopolymers.

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