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Peter M Eimon et al.
Nature communications, 9(1), 219-219 (2018-01-18)
Neurological drugs are often associated with serious side effects, yet drug screens typically focus only on efficacy. We demonstrate a novel paradigm utilizing high-throughput in vivo electrophysiology and brain activity patterns (BAPs). A platform with high sensitivity records local field potentials (LFPs)...
BanuPriya Sridharan et al.
PloS one, 10(12), e0141479-e0141479 (2016-01-01)
There are a variety of exciting hydrogel technologies being explored for cartilage regenerative medicine. Our overall goal is to explore whether using stem cells in an aggregate form may be advantageous in these applications. 3D stem cell aggregates hold great...
Design of a toxicity biosensor based on Aliivibrio fischeri entrapped in a disposable card
Jouanneau, S, et al.
Environmental Science and Pollution Research International, 23(5), 4340-4345 (2016)
Yuko Sato et al.
Development (Cambridge, England), 146(19) (2019-10-02)
Histone post-translational modifications are key gene expression regulators, but their rapid dynamics during development remain difficult to capture. We applied a Fab-based live endogenous modification labeling technique to monitor the changes in histone modification levels during zygotic genome activation (ZGA)...
D L Bark et al.
Journal of biomechanics, 50, 50-55 (2016-11-27)
Cardiovascular development is influenced by the flow-induced stress environment originating from cardiac biomechanics. To characterize the stress environment, it is necessary to quantify flow and pressure. Here, we quantify the flow field in a developing zebrafish heart during the looping...
Xiaogang Wang et al.
Journal of the mechanical behavior of biomedical materials, 114, 104150-104150 (2020-11-21)
Hydrogels have seen widespread application across biomedical sciences and there is considerable interest in using hydrogels, including agarose, for creating in vitro three-dimensional environments to grow cells and study mechanobiology and mechanotransduction. Recent advances in the preparation of agarose gels...
Lesley D Schurman et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 39(30), 5949-5965 (2019-05-28)
Diacylglycerol lipase-α (DAGL-α), the principal biosynthetic enzyme of the endogenous cannabinoid 2-arachidonylglycerol (2-AG) on neurons, plays a key role in CB1 receptor-mediated synaptic plasticity and hippocampal neurogenesis, but its contribution to global hippocampal-mediated processes remains unknown. Thus, the present study...
Ciana Deveau et al.
PLoS genetics, 16(6), e1008869-e1008869 (2020-06-23)
We investigate mutations in trβ2, a splice variant of thrb, identifying changes in function, structure, and behavior in larval and adult zebrafish retinas. Two N-terminus CRISPR mutants were identified. The first is a 6BP+1 insertion deletion frameshift resulting in a...
Alexander Mietke et al.
Biophysical journal, 109(10), 2023-2036 (2015-11-21)
Cell stiffness is a sensitive indicator of physiological and pathological changes in cells, with many potential applications in biology and medicine. A new method, real-time deformability cytometry, probes cell stiffness at high throughput by exposing cells to a shear flow...
Gregory J Anthony et al.
Physics in medicine and biology, 64(9), 095023-095023 (2019-03-29)
Histotripsy is a therapeutic ultrasound modality under development to liquefy tissue mechanically via bubble clouds. Image guidance of histotripsy requires both quantification of the bubble cloud activity and accurate delineation of the treatment zone. In this study, magnetic resonance (MR)...
Valentina Baena et al.
Endocrinology, 161(7) (2020-05-10)
Meiotic arrest and resumption in mammalian oocytes are regulated by 2 opposing signaling proteins in the cells of the surrounding follicle: the guanylyl cyclase natriuretic peptide receptor 2 (NPR2), and the luteinizing hormone receptor (LHR). NPR2 maintains a meiosis-inhibitory level...
Jeffrey W Simkins et al.
Journal of magnetic resonance (San Diego, Calif. : 1997), 293, 123-133 (2018-06-26)
19F magnetic resonance has been used in the medical field for quantifying oxygenation in blood, tissues, and tumors. The 19F NMR oximetry technique exploits the affinity of molecular oxygen for liquid fluorocarbon phases, and the resulting linear dependence of 19F...
Chiharu Tani et al.
Applied and environmental microbiology, 75(22), 7044-7050 (2009-09-22)
Arbuscular mycorrhizal (AM) fungi benefit their host plants by supplying phosphate obtained from the soil. Polyphosphate is thought to act as the key intermediate in this process, but little is currently understood about how polyphosphate is synthesized or translocated within...
Feng Xu et al.
PloS one, 6(4), e19344-e19344 (2011-05-10)
Decellularization and cellularization of organs have emerged as disruptive methods in tissue engineering and regenerative medicine. Porous hydrogel scaffolds have widespread applications in tissue engineering, regenerative medicine and drug discovery as viable tissue mimics. However, the existing hydrogel fabrication techniques...
Ning Zhao et al.
Nature communications, 10(1), 2947-2947 (2019-07-05)
To expand the toolbox of imaging in living cells, we have engineered a single-chain variable fragment binding the linear HA epitope with high affinity and specificity in vivo. The resulting probe, called the HA frankenbody, can light up in multiple...
Mostafa Ghannad-Rezaie et al.
Nature communications, 10(1), 2620-2620 (2019-06-15)
Conventional drug screens and treatments often ignore the underlying complexity of brain network dysfunctions, resulting in suboptimal outcomes. Here we ask whether we can correct abnormal functional connectivity of the entire brain by identifying and combining multiple neuromodulators that perturb connectivity...
Steven Ceto et al.
Cell stem cell, 27(3), 430-440 (2020-08-08)
Neural stem/progenitor cell (NSPC) grafts can integrate into sites of spinal cord injury (SCI) and generate neuronal relays across lesions that can provide functional benefit. To determine if and how grafts become synaptically organized and connect with host systems, we...
Sonia Samtani et al.
Investigative ophthalmology & visual science, 50(11), 5098-5106 (2009-06-12)
Doxycycline, a broad-spectrum antibiotic, has certain antiangiogenic properties and can inhibit matrix metalloproteinases (MMPs/gelatinases). The authors investigated the effects of doxycycline on choroidal neovascularization (CNV) and regulation of MMP-2 and -9 and pigment epithelium-derived factor (PEDF). Doxycycline was orally administered...
Surfactant-induced coagulation of agarose from aqueous extract of Gracilaria dura seaweed as an energy-efficient alternative to the conventional freeze--thaw process
Meena R, et al.
Royal Society of Chemistry Advances, 4(53), 28093-28098 (2014)
Mahmoud Affi et al.
Analytical and bioanalytical chemistry, 408(30), 8761-8770 (2016-04-05)
Water quality and water management are worldwide issues. The analysis of pollutants and in particular, heavy metals, is generally conducted by sensitive but expensive physicochemical methods. Other alternative methods of analysis, such as microbial biosensors, have been developed for their...
Andrew Miri et al.
Journal of neurophysiology, 105(2), 964-980 (2010-11-19)
The advent of methods for optical imaging of large-scale neural activity at cellular resolution in behaving animals presents the problem of identifying behavior-encoding cells within the resulting image time series. Rapid and precise identification of cells with particular neural encoding...
Andrew Advani et al.
PloS one, 6(10), e24695-e24695 (2011-10-11)
Rarefaction of the renal microvasculature correlates with declining kidney function. However, current technologies commonly used for its evaluation are limited by their reliance on endothelial cell antigen expression and assessment in two dimensions. We set out to establish a widely...
Mei Wang et al.
The Journal of cell biology, 219(9) (2020-07-24)
How astounding neuronal diversity arises from variable cell lineages in vertebrates remains mostly elusive. By in vivo lineage tracing of ∼1,000 single zebrafish retinal progenitors, we identified a repertoire of subtype-specific stereotyped neurogenic lineages. Remarkably, within these stereotyped lineages, GABAergic...
Sargon Gross-Thebing et al.
Science advances, 6(49) (2020-12-06)
The biophysical and biochemical properties of live tissues are important in the context of development and disease. Methods for evaluating these properties typically involve destroying the tissue or require specialized technology and complicated analyses. Here, we present a novel, noninvasive...
Melissa C Skala et al.
Nano letters, 8(10), 3461-3467 (2008-09-05)
Molecular imaging is a powerful tool for investigating disease processes and potential therapies in both in vivo and in vitro systems. However, high resolution molecular imaging has been limited to relatively shallow penetration depths that can be accessed with microscopy....
Luis M Polo et al.
Cell reports, 26(3), 573-581 (2019-01-17)
XRCC1 accelerates repair of DNA single-strand breaks by acting as a scaffold protein for the recruitment of Polβ, LigIIIα, and end-processing factors, such as PNKP and APTX. XRCC1 itself is recruited to DNA damage through interaction of its central BRCT...
Stephanie Möllmert et al.
Biophysical journal, 118(2), 448-463 (2019-12-25)
Severe injury to the mammalian spinal cord results in permanent loss of function due to the formation of a glial-fibrotic scar. Both the chemical composition and the mechanical properties of the scar tissue have been implicated to inhibit neuronal regrowth...
Jérôme Bove et al.
Plant physiology, 147(4), 1646-1658 (2008-05-30)
The delivery of cell wall material and membrane to growing plant cell surfaces requires the spatial and temporal coordination of secretory vesicle trafficking. Given the small size of vesicles, their dynamics is difficult to quantify. To quantitatively analyze vesicle dynamics...
Carley N McCutchen et al.
Journal of biomechanics, 64, 49-58 (2017-10-08)
Cells sense and respond to mechanical loads in a process called mechanotransduction. These processes are disrupted in the chondrocytes of cartilage during joint disease. A key driver of cellular mechanotransduction is the stiffness of the surrounding matrix. Many cells are...
Suraj U Hettiarachchi et al.
Science translational medicine, 12(567) (2020-10-30)
Idiopathic pulmonary fibrosis (IPF) is a lethal disease with an average life expectancy of 3 to 5 years. IPF is characterized by progressive stiffening of the lung parenchyma due to excessive deposition of collagen, leading to gradual failure of gas...
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