Showing 1-30 of 37 results for "

a0701

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Karina Scalise et al.
Journal of neurophysiology, 116(5), 2067-2080 (2016-11-03)
Although most studies of the cerebellum have been conducted in mammals, cerebellar circuitry is highly conserved across vertebrates, suggesting that studies of simpler systems may be useful for understanding cerebellar function. The larval zebrafish is particularly promising in this regard...
Extracting cell stiffness from real-time deformability cytometry: theory and experiment
Mietke A, et al.
Biophysical Journal, 109(10), 2023-2036 (2015)
Andrew Miri et al.
Journal of neurophysiology, 105(2), 964-980 (2010-11-19)
The advent of methods for optical imaging of large-scale neural activity at cellular resolution in behaving animals presents the problem of identifying behavior-encoding cells within the resulting image time series. Rapid and precise identification of cells with particular neural encoding...
Mei Wang et al.
The Journal of cell biology, 219(9) (2020-07-24)
How astounding neuronal diversity arises from variable cell lineages in vertebrates remains mostly elusive. By in vivo lineage tracing of ∼1,000 single zebrafish retinal progenitors, we identified a repertoire of subtype-specific stereotyped neurogenic lineages. Remarkably, within these stereotyped lineages, GABAergic...
Andrew Advani et al.
PloS one, 6(10), e24695-e24695 (2011-10-11)
Rarefaction of the renal microvasculature correlates with declining kidney function. However, current technologies commonly used for its evaluation are limited by their reliance on endothelial cell antigen expression and assessment in two dimensions. We set out to establish a widely...
Surfactant-induced coagulation of agarose from aqueous extract of Gracilaria dura seaweed as an energy-efficient alternative to the conventional freeze--thaw process
Meena R, et al.
Royal Society of Chemistry Advances, 4(53), 28093-28098 (2014)
Anurima Majumder et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 35(24), 9225-9235 (2015-06-19)
Despite the expression of homologous phototransduction components, the molecular basis for differences in light-evoked responses between rod and cone photoreceptors remains unclear. We examined the role of cGMP phosphodiesterase (PDE6) in this difference by expressing cone PDE6 (PDE6C) in rd1/rd1...
Kavitha Sivasubramaiyan et al.
Journal of biomedical science, 16, 88-88 (2009-09-24)
Trophoblast differentiation and formation of the placenta are important events linked to post-implantation embryonic development. Models mimicking the biology of trophoblast differentiation in a post-implantation maternal microenvironment are needed for understanding disorders like placental-ischemia or for applications in drug-screening, and...
M Cecilia Cirio et al.
PloS one, 6(4), e18858-e18858 (2011-04-29)
In the vertebrate embryo, the kidney is derived from the intermediate mesoderm. The LIM-class homeobox transcription factor lhx1 is expressed early in the intermediate mesoderm and is one of the first genes to be expressed in the nephric mesenchyme. In...
Mahmoud Affi et al.
Analytical and bioanalytical chemistry, 408(30), 8761-8770 (2016-04-05)
Water quality and water management are worldwide issues. The analysis of pollutants and in particular, heavy metals, is generally conducted by sensitive but expensive physicochemical methods. Other alternative methods of analysis, such as microbial biosensors, have been developed for their...
Sargon Gross-Thebing et al.
Science advances, 6(49) (2020-12-06)
The biophysical and biochemical properties of live tissues are important in the context of development and disease. Methods for evaluating these properties typically involve destroying the tissue or require specialized technology and complicated analyses. Here, we present a novel, noninvasive...
Melissa C Skala et al.
Nano letters, 8(10), 3461-3467 (2008-09-05)
Molecular imaging is a powerful tool for investigating disease processes and potential therapies in both in vivo and in vitro systems. However, high resolution molecular imaging has been limited to relatively shallow penetration depths that can be accessed with microscopy....
Luis M Polo et al.
Cell reports, 26(3), 573-581 (2019-01-17)
XRCC1 accelerates repair of DNA single-strand breaks by acting as a scaffold protein for the recruitment of Polβ, LigIIIα, and end-processing factors, such as PNKP and APTX. XRCC1 itself is recruited to DNA damage through interaction of its central BRCT...
Suraj U Hettiarachchi et al.
Science translational medicine, 12(567) (2020-10-30)
Idiopathic pulmonary fibrosis (IPF) is a lethal disease with an average life expectancy of 3 to 5 years. IPF is characterized by progressive stiffening of the lung parenchyma due to excessive deposition of collagen, leading to gradual failure of gas...
Carley N McCutchen et al.
Journal of biomechanics, 64, 49-58 (2017-10-08)
Cells sense and respond to mechanical loads in a process called mechanotransduction. These processes are disrupted in the chondrocytes of cartilage during joint disease. A key driver of cellular mechanotransduction is the stiffness of the surrounding matrix. Many cells are...
Jérôme Bove et al.
Plant physiology, 147(4), 1646-1658 (2008-05-30)
The delivery of cell wall material and membrane to growing plant cell surfaces requires the spatial and temporal coordination of secretory vesicle trafficking. Given the small size of vesicles, their dynamics is difficult to quantify. To quantitatively analyze vesicle dynamics...
Spencer S Gang et al.
PLoS pathogens, 13(10), e1006675-e1006675 (2017-10-11)
Parasitic nematodes infect over 1 billion people worldwide and cause some of the most common neglected tropical diseases. Despite their prevalence, our understanding of the biology of parasitic nematodes has been limited by the lack of tools for genetic intervention....
Christopher J Folts et al.
PLoS biology, 14(12), e1002583-e1002583 (2016-12-16)
Neurodegenerative lysosomal storage disorders (LSDs) are severe and untreatable, and mechanisms underlying cellular dysfunction are poorly understood. We found that toxic lipids relevant to three different LSDs disrupt multiple lysosomal and other cellular functions. Unbiased drug discovery revealed several structurally...
Stephanie Möllmert et al.
Biophysical journal, 118(2), 448-463 (2019-12-25)
Severe injury to the mammalian spinal cord results in permanent loss of function due to the formation of a glial-fibrotic scar. Both the chemical composition and the mechanical properties of the scar tissue have been implicated to inhibit neuronal regrowth...
BanuPriya Sridharan et al.
PloS one, 10(12), e0141479-e0141479 (2016-01-01)
There are a variety of exciting hydrogel technologies being explored for cartilage regenerative medicine. Our overall goal is to explore whether using stem cells in an aggregate form may be advantageous in these applications. 3D stem cell aggregates hold great...
Peter M Eimon et al.
Nature communications, 9(1), 219-219 (2018-01-18)
Neurological drugs are often associated with serious side effects, yet drug screens typically focus only on efficacy. We demonstrate a novel paradigm utilizing high-throughput in vivo electrophysiology and brain activity patterns (BAPs). A platform with high sensitivity records local field potentials (LFPs)...
Ning Zhao et al.
Nature communications, 10(1), 2947-2947 (2019-07-05)
To expand the toolbox of imaging in living cells, we have engineered a single-chain variable fragment binding the linear HA epitope with high affinity and specificity in vivo. The resulting probe, called the HA frankenbody, can light up in multiple...
Feng Xu et al.
PloS one, 6(4), e19344-e19344 (2011-05-10)
Decellularization and cellularization of organs have emerged as disruptive methods in tissue engineering and regenerative medicine. Porous hydrogel scaffolds have widespread applications in tissue engineering, regenerative medicine and drug discovery as viable tissue mimics. However, the existing hydrogel fabrication techniques...
Steven Ceto et al.
Cell stem cell, 27(3), 430-440 (2020-08-08)
Neural stem/progenitor cell (NSPC) grafts can integrate into sites of spinal cord injury (SCI) and generate neuronal relays across lesions that can provide functional benefit. To determine if and how grafts become synaptically organized and connect with host systems, we...
Mostafa Ghannad-Rezaie et al.
Nature communications, 10(1), 2620-2620 (2019-06-15)
Conventional drug screens and treatments often ignore the underlying complexity of brain network dysfunctions, resulting in suboptimal outcomes. Here we ask whether we can correct abnormal functional connectivity of the entire brain by identifying and combining multiple neuromodulators that perturb connectivity...
Valentina Baena et al.
Endocrinology, 161(7) (2020-05-10)
Meiotic arrest and resumption in mammalian oocytes are regulated by 2 opposing signaling proteins in the cells of the surrounding follicle: the guanylyl cyclase natriuretic peptide receptor 2 (NPR2), and the luteinizing hormone receptor (LHR). NPR2 maintains a meiosis-inhibitory level...
Yuko Sato et al.
Development (Cambridge, England), 146(19) (2019-10-02)
Histone post-translational modifications are key gene expression regulators, but their rapid dynamics during development remain difficult to capture. We applied a Fab-based live endogenous modification labeling technique to monitor the changes in histone modification levels during zygotic genome activation (ZGA)...
Sonia Samtani et al.
Investigative ophthalmology & visual science, 50(11), 5098-5106 (2009-06-12)
Doxycycline, a broad-spectrum antibiotic, has certain antiangiogenic properties and can inhibit matrix metalloproteinases (MMPs/gelatinases). The authors investigated the effects of doxycycline on choroidal neovascularization (CNV) and regulation of MMP-2 and -9 and pigment epithelium-derived factor (PEDF). Doxycycline was orally administered...
Ciana Deveau et al.
PLoS genetics, 16(6), e1008869-e1008869 (2020-06-23)
We investigate mutations in trβ2, a splice variant of thrb, identifying changes in function, structure, and behavior in larval and adult zebrafish retinas. Two N-terminus CRISPR mutants were identified. The first is a 6BP+1 insertion deletion frameshift resulting in a...
Xiaogang Wang et al.
Journal of the mechanical behavior of biomedical materials, 114, 104150-104150 (2020-11-21)
Hydrogels have seen widespread application across biomedical sciences and there is considerable interest in using hydrogels, including agarose, for creating in vitro three-dimensional environments to grow cells and study mechanobiology and mechanotransduction. Recent advances in the preparation of agarose gels...