Showing 1-30 of 140 results for "

a0884

"
Zhongli Yang et al.
Neuroscience letters, 555, 215-219 (2013-10-01)
Recently, we reported that several polymorphisms and haplotypes in the choline acetyltransferase gene (ChAT) are associated with nicotine dependence (ND). Of them, SNP rs1880676 is of particular interest because: (1) it is a non-synonymous variant located in the coding region...
Kevin Guo et al.
Analytical chemistry, 81(10), 3919-3932 (2009-03-25)
We report a new quantitative metabolome profiling technique based on differential (12)C-/(13)C-isotope dansylation labeling of metabolites, fast liquid chromatography (LC) separation and electrospray ionization Fourier-transform ion cyclotron resonance mass spectrometry (ESI-FTICR MS) detection. An isotope reagent, (13)C-dansyl chloride, can be...
J D Shoemaker et al.
Journal of chromatography, 562(1-2), 125-138 (1991-01-02)
Eighty-five clinical urine samples and nineteen urine samples previously found by other laboratories to suggest genetic metabolic defects were prepared for trimethylsilylation by treatment with urease, followed by azeotropic dehydration. The "Target Analyte Search" program provided with the VG Trio...
Asparagine levels in the bone marrow of patients with acute lymphoblastic leukemia during asparaginase therapy.
Martina Chiu et al.
Pediatric blood & cancer, 60(11), 1915-1915 (2013-08-01)
J C Filho et al.
Clinical nutrition (Edinburgh, Scotland), 16(6), 299-305 (2006-07-18)
Muscle biopsy and blood samples were simultaneously collected from 27 healthy subjects for determination of free amino acid (AA) in plasma, muscle and erythrocytes (RBC) by reverse-phase high performance liquid chromatography and related their concentrations to deoxyribonucleic acid (DNA), muscle...
Beata Mickiewicz et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 33(1), 71-77 (2014-10-07)
Joint injuries and subsequent osteoarthritis (OA) are the leading causes of chronic joint disease. In this work, we explore the possibility of applying magnetic resonance spectroscopy-based metabolomics to detect host responses to an anterior cruciate ligament (ACL) reconstruction injury in...
Karin Mohrmann et al.
Cancer chemotherapy and pharmacology, 56(4), 344-350 (2005-05-06)
Breast cancer resistance protein (BCRP/ABCG2) is an ATP-binding cassette (ABC) multidrug transporter that confers resistance to various anticancer drugs like topotecan and mitoxantrone. To obtain more insight in its cellular functioning, we investigated phosphorylation and N-linked glycosylation of BCRP. In...
Lijun Zhou et al.
Nature, 506(7486), 116-120 (2013-11-19)
Splicing of precursor messenger RNA (pre-mRNA) in eukaryotic cells is carried out by the spliceosome, which consists of five small nuclear ribonucleoproteins (snRNPs) and a number of accessory factors and enzymes. Each snRNP contains a ring-shaped subcomplex of seven proteins...
Louise T Henriksen et al.
British journal of haematology, 166(2), 213-220 (2014-04-08)
L-asparaginase is an important drug in the treatment of childhood acute lymphoblastic leukaemia (ALL). Cerebrospinal fluid (CSF) asparagine depletion is considered a marker of asparaginase effect in the central nervous system (CNS) and may play a role in CNS-directed anti-leukaemia...
Xiaolang Cai et al.
Wei sheng wu xue bao = Acta microbiologica Sinica, 52(12), 1467-1476 (2013-03-06)
Mycobacterium tuberculosis can survive inside host cells for a long time. To elucidate the acid adaptation mechanism mediated by the asparagine (Asn) metabolic pathway, the activity of asparaginase (AnsA) and growth of mycobacteria were characterized. The nonpathogenic M. bovis BCG...
Stefani R Mladenova et al.
FEBS letters, 588(9), 1808-1812 (2014-04-02)
The human pathogen Staphylococcus aureus is an asparagine prototroph despite its genome not encoding an asparagine synthetase. S. aureus does use an asparaginyl-tRNA synthetase (AsnRS) to directly ligate asparagine to tRNA(Asn). The S. aureus genome also codes for one aspartyl-tRNA...
Yang Hai et al.
Biochemistry, 52(51), 9294-9309 (2013-11-23)
The crystal structure of formiminoglutamase from Trypanosoma cruzi (TcFIGase) is reported at 1.85 Å resolution. Although the structure of this enzyme was previously determined by the Structural Genomics of Pathogenic Protozoa Consortium (PDB accession code 2A0M), this structure was determined...
E M Michalak et al.
Cell death and differentiation, 15(6), 1019-1029 (2008-02-09)
The ability of p53 to induce apoptosis in cells with damaged DNA is thought to contribute greatly to its tumour suppressor function. P53 has been proposed to induce apoptosis via numerous transcriptional targets or even by direct cytoplasmic action. Two...
Peihan Orestes et al.
Diabetes, 62(11), 3828-3838 (2013-07-10)
It has been established that Ca(V)3.2 T-type voltage-gated calcium channels (T-channels) play a key role in the sensitized (hyperexcitable) state of nociceptive sensory neurons (nociceptors) in response to hyperglycemia associated with diabetes, which in turn can be a basis for...
Richard C Grandison et al.
Nature, 462(7276), 1061-1064 (2009-12-04)
Dietary restriction extends healthy lifespan in diverse organisms and reduces fecundity. It is widely assumed to induce adaptive reallocation of nutrients from reproduction to somatic maintenance, aiding survival of food shortages in nature. If this were the case, long life...
Indalecio Quesada-Soriano et al.
Biochimica et biophysica acta, 1844(9), 1427-1436 (2014-05-06)
The glutathione S-transferase from Plasmodium falciparum presents distinct features which are absent from mammalian GST isoenzyme counterparts. Most apparent among these are the ability to tetramerize and the presence of a flexible loop. The loop, situated between the 113-119 residues...
Wolfram Gronwald et al.
Kidney international, 79(11), 1244-1253 (2011-03-11)
Autosomal dominant polycystic kidney disease (ADPKD) is a frequent cause of kidney failure; however, urinary biomarkers for the disease are lacking. In a step towards identifying such markers, we used multidimensional-multinuclear nuclear magnetic resonance (NMR) spectroscopy with support vector machine-based...
H C Curtius et al.
Journal of chromatography, 112, 523-531 (1975-10-29)
A specific and very sensitive method for the determination of 5-hydroxy-tryptophol (5-HTOL) and 5-methoxytryptophol (5-MTOL) in extracts from human cerebrospinal fluid (CSF) involving the use of mass fragmentography and pentafluoropriopionyl derivatives is described. 5-HTOL and 5-MTOL were determined in human...
Marie Pancera et al.
PloS one, 8(2), e55701-e55701 (2013-02-23)
Dozens of broadly neutralizing HIV-1 antibodies have been isolated in the last few years from the sera of HIV-1-infected individuals. Only a limited number of regions on the HIV-1 spike, however, are recognized by these antibodies. One of these regions...
Benjamin J Des Soye et al.
Cell chemical biology, 26(12), 1743-1754 (2019-11-11)
The site-specific incorporation of non-canonical amino acids (ncAAs) into proteins via amber suppression provides access to novel protein properties, structures, and functions. Historically, poor protein expression yields resulting from release factor 1 (RF1) competition has limited this technology. To address...
A N Fonteh et al.
Amino acids, 32(2), 213-224 (2006-10-13)
Our aim was to determine changes in free amino acid (FAA) and dipeptide (DP) concentrations in probable Alzheimer's disease (pAD) subjects compared with control (CT) subjects using liquid chromatography and electrospray ionization tandem mass spectrometry (LCMS2). We recruited gender- and...
David S Wishart et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 871(2), 164-173 (2008-05-27)
With continuing improvements in analytical technology and an increased interest in comprehensive metabolic profiling of biofluids and tissues, there is a growing need to develop comprehensive reference resources for certain clinically important biofluids, such as blood, urine and cerebrospinal fluid...
G Alfredsson et al.
Psychopharmacology, 99(3), 322-327 (1989-01-01)
Twenty-four acutely ill schizophrenic patients (DSM-III-R), 18-42 years old, were treated for 6 weeks with sulpiride. Sulpiride was administered in three different daily dosages (starting with 400, 800 or 1200 mg) according to a double blind randomized administration schedule. The...
T Fujita et al.
Clinica chimica acta; international journal of clinical chemistry, 287(1-2), 99-109 (1999-10-06)
Pipecolic acid is regarded as a gamma-aminobutyric acid receptor agonist. Stereochemical studies of pipecolic acid were performed in patients with chronic liver diseases. Plasma D- and L-pipecolic acid were significantly elevated in 15 liver cirrhotic patients with no history of...
Johanna Chiche et al.
Cell metabolism, 29(6), 1243-1257 (2019-03-05)
Diffuse large B cell lymphoma (DLBCL) is a heterogeneous disease treated with anti-CD20-based immuno-chemotherapy (R-CHOP). We identified that low levels of GAPDH predict a poor response to R-CHOP treatment. Importantly, we demonstrated that GAPDHlow lymphomas use OxPhos metabolism and rely...
Maria Trott et al.
PloS one, 9(5), e97478-e97478 (2014-05-16)
HIV neutralizing antibodies (nAbs) represent an important tool in view of prophylactic and therapeutic applications for HIV-1 infection. Patients chronically infected by HIV-1 represent a valuable source for nAbs. HIV controllers, including long-term non-progressors (LTNP) and elite controllers (EC), represent...
Guijie Ren et al.
Canadian journal of microbiology, 59(9), 641-644 (2013-09-10)
To explore the effects of amino acids Gln and Asn within the specific fusion domain of fusion (F) protein on the specific membrane fusion in Newcastle disease virus (NDV), the mutants Q204E-Q205E and N245D were constructed in the specific fusion...
Velia Minicozzi et al.
The Journal of biological chemistry, 289(16), 11242-11252 (2014-03-04)
In this work we present and compare the results of extensive molecular dynamics simulations of model systems comprising an Aβ1-40 peptide in water in interaction with short peptides (β-sheet breakers) mimicking the 17-21 region of the Aβ1-40 sequence. Various systems...
Silvia Pérez-Magariño et al.
Journal of agricultural and food chemistry, 63(23), 5670-5681 (2015-06-02)
In sparkling wines, mannoproteins released during yeast autolysis largely affect their final quality. This process is very slow and may take several months. The aim of this work was to study the effect of several commercial dry yeast autolysates on...
Beatrice Bocca et al.
Annali dell'Istituto superiore di sanita, 41(2), 197-203 (2005-10-26)
The haematic concentration of 26 metals and the oxidative damage in 60 patients (20 males and 40 females) affected by Alzheimer's disease and 44 healthy individuals (33 males and 11 females) were compared. In patients, the following significant (p <...