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Showing 1-30 of 126 results for "A2576" within Papers
Protein crystallization in agarose gel with high strength: developing an automated system for protein crystallographic processes
Sugiyama S, et al.
Japanese Journal of Applied Physics, 48(7R), 075502-075502 (2009)
Jia Liu et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(17), 6694-6699 (2013-04-10)
Seamless and minimally invasive integration of 3D electronic circuitry within host materials could enable the development of materials systems that are self-monitoring and allow for communication with external environments. Here, we report a general strategy for preparing ordered 3D interconnected
Nobuyuki Tanaka et al.
PloS one, 12(4), e0173647-e0173647 (2017-04-06)
The geometrical confinement of small cell colonies gives differential cues to cells sitting at the periphery versus the core. To utilize this effect, for example to create spatially graded differentiation patterns of human mesenchymal stem cells (hMSCs) in vitro or
R Alvarez et al.
Journal of virology, 82(1), 471-486 (2007-10-19)
Understanding why human immunodeficiency virus (HIV) preferentially infects some CD4(+) CD45RO(+) memory T cells has implications for antiviral immunity and pathogenesis. We report that differential expression of a novel secreted factor, ps20, previously implicated in tissue remodeling, may underlie why
Microcasting with agarose gel via degassed polydimethylsiloxane molds for repellency-guided cell patterning
Tanaka N, et al.
Royal Society of Chemistry Advances, 6(60), 54754-54762 (2016)
Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993), 74(6), 16-23 (2013-01-09)
A scheme of isolation and purification of the enzyme with alpha-L-rhamnosidase activity from culture liquid Cryptococcus albidus 1001 has been developed. It included fractionation by ammonium sulfate and chromatography on TSK-gels Toyopearl HW-60, Fractogel TSK DEAE-650-s and Sepharose 6B. The
Rawand Masoud et al.
Cell reports. Medicine, 1(8), 100143-100143 (2020-12-10)
Mitochondrial respiration (oxidative phosphorylation, OXPHOS) is an emerging target in currently refractory cancers such as pancreatic ductal adenocarcinoma (PDAC). However, the variability of energetic metabolic adaptations between PDAC patients has not been assessed in functional investigations. In this work, we
Nathan A Baird et al.
PloS one, 3(10), e3376-e3376 (2008-10-15)
Single nucleotide polymorphism (SNP) discovery and genotyping are essential to genetic mapping. There remains a need for a simple, inexpensive platform that allows high-density SNP discovery and genotyping in large populations. Here we describe the sequencing of restriction-site associated DNA
Xianbao He et al.
PLoS pathogens, 9(8), e1003569-e1003569 (2013-09-07)
The sst1, "supersusceptibility to tuberculosis," locus has previously been shown to be a genetic determinant of host resistance to infection with the intracellular pathogen, Mycobacterium tuberculosis. Chlamydia pneumoniae is an obligate intracellular bacterium associated with community acquired pneumonia, and chronic
Purification of recombinant protein derived from the baculovirus expression system using glutathione affinity agarose.
E J Sorscher et al.
Methods in molecular biology (Clifton, N.J.), 39, 337-348 (1995-01-01)
Minglin Sun et al.
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery, 27(1), 89-94 (2013-02-23)
To explore the effect of rolling compression loading bioreactor on chondrogenesis of rabbit bone marrow mesenchymal stem cells (BMSCs) with different loading parameters. BMSCs were isolated from New Zealand rabbits, aged 2.5 months. BMSCs at passage 3 were used to
J Porath
Journal of molecular recognition : JMR, 3(3), 123-127 (1990-06-01)
Protein adsorption and retention data collected from recent chromatographic studies on hydrophilic gels substituted with chelate-bonded metal ions are discussed. Attempts are made to interpret the adsorption behavior in terms of molecular events caused by the affinity for the immobilized
Simple agarose micro-confinement array and machine-learning-based classification for analyzing the patterned differentiation of mesenchymal stem cells
Tanaka N, et al.
PLoS ONE, 12(4), e0173647-e0173647 (2017)
Lan Zhao et al.
Biomedical chromatography : BMC, 27(9), 1089-1091 (2013-05-18)
To study the leakage at different solution pH values, IgG Sepharose 6FFĀ®, a commercially available immunoadsorbent, was used as a model. The leaked substance consists of three parts: (1) ligands and its fragments; (2) ligands plus matrix fragments in which
Hu Liu et al.
Journal of chromatography. A, 1344, 59-65 (2014-04-29)
This work proposed an integrative method of protein refolding and purification by like-charged resin facilitated refolding and metal-chelate affinity adsorption. Hexahistidine-tagged enhanced green fluorescence protein (EGFP) was overexpressed in Escherichia coli as inclusion bodies (IBs), and then the protein was
Gianluca VadalĆ et al.
Spine, 38(6), E319-E324 (2013-01-18)
Descriptive anatomical study on ovine and human cadaveric lumbar spinal segments. To describe the alternative transpedicular approach to deliver therapeutic agents into intervertebral disc (IVD). The present delivery approach of therapeutic agents (growth factors/cells/hydrogels) within the IVD is through injection
Shailabh Kumar et al.
Nature communications, 12(1), 6018-6018 (2021-10-16)
Mosquito bites transmit a number of pathogens via salivary droplets deposited during blood-feeding, resulting in potentially fatal diseases. Little is known about the genomic content of these nanodroplets, including the transmission dynamics of live pathogens. Here we introduce Vectorchip, a
Paul Szyszka et al.
Frontiers in systems neuroscience, 2, 3-3 (2008-10-30)
The insect mushroom bodies are higher-order brain centers and critical for odor learning. We investigated experience dependent plasticity of their intrinsic neurons, the Kenyon cells (KCs). Using calcium imaging, we recorded KC responses and investigated non-associative plasticity by applying repeated
Tatsuya Kawai et al.
Acta radiologica (Stockholm, Sweden : 1987), 54(8), 954-960 (2013-04-25)
The effects of a tin filter on virtual non-enhanced (VNE) images created by dual-energy CT have not been well evaluated. To compare the accuracy of VNE images between those with and without a tin filter. Two different types of columnar
Yolanda Schaerli
Bio-protocol, 8(13) (2018-07-20)
High-throughput screening of a DNA library expressed in a bacterial population for identifying potentially rare members displaying a property of interest is a crucial step for success in many experiments such as directed evolution of proteins and synthetic circuits and
Joshua Schindel et al.
International journal of radiation oncology, biology, physics, 86(2), 387-393 (2013-02-26)
To present a novel marker-flange, addressing source-reconstruction uncertainties due to the artifacts of a titanium intracavitary applicator used for magnetic resonance imaging (MRI)-guided high-dose-rate (HDR) brachytherapy (BT); and to evaluate 7 different MRI marker agents used for interstitial prostate BT
Jeremy J Flint et al.
NeuroImage, 219, 116997-116997 (2020-06-04)
Since its first description and development in the late 20th century, diffusion magnetic resonance imaging (dMRI) has proven useful in describing the microstructural details of biological tissues. Signal generated from the protons of water molecules undergoing Brownian motion produces contrast
G Duro et al.
Journal of chromatography, 618(1-2), 95-104 (1993-08-25)
Agarose gel electrophoresis is a powerful technique for the separation of nucleic acids on the basis of their size and conformation. The development of methods to recover size-fractionated nucleic acids molecules from agarose gels has greatly facilitated recombinant DNA technologies.
Mostafa Ghannad-Rezaie et al.
Nature communications, 10(1), 2620-2620 (2019-06-15)
Conventional drug screens and treatments often ignore the underlying complexity of brainĀ network dysfunctions, resulting in suboptimal outcomes. Here we ask whether we can correct abnormal functional connectivity of the entire brain by identifying and combining multiple neuromodulators that perturb connectivity
Ian G Cowell et al.
Mutagenesis, 26(2), 253-260 (2010-11-12)
The ability to detect and quantify specific DNA adducts benefits genome stability research, drug development and the evaluation of environmental mutagens. The trapped in agarose DNA immunostaining (TARDIS) assay was developed as a means of detecting and quantifying melphalan and
Michael E Todhunter et al.
Current protocols in chemical biology, 8(3), 147-178 (2016-09-14)
Tissues are the organizational units of function in metazoan organisms. Tissues comprise an assortment of cellular building blocks, soluble factors, and extracellular matrix (ECM) composed into specific three-dimensional (3-D) structures. The capacity to reconstitute tissues in vitro with the structural
Feng Xu et al.
PloS one, 6(4), e19344-e19344 (2011-05-10)
Decellularization and cellularization of organs have emerged as disruptive methods in tissue engineering and regenerative medicine. Porous hydrogel scaffolds have widespread applications in tissue engineering, regenerative medicine and drug discovery as viable tissue mimics. However, the existing hydrogel fabrication techniques
Haruka Koga et al.
Biofabrication, 5(1), 015010-015010 (2013-01-11)
Micropatterning is useful for regulating culture environments. We developed a highly efficient near-infrared-(NIR)-responsive gel and established a new technique that enables cell patterning by NIR irradiation. As a new culture substratum, we designed a tissue culture plate that was coated
F H Kirkpatrick et al.
Electrophoresis, 14(4), 349-354 (1993-04-01)
Properties of agarose potentially relevant to PFGE (pulsed-field gel electrophoresis) are reviewed, and some new information is presented. Agarose polymers appear to have molecular weights in the range of 100,000 to 200,000 Da, but this is not tightly related to
Wen-Jun Han et al.
The Journal of biological chemistry, 288(13), 9519-9531 (2013-02-05)
Agarase hydrolyzes agarose into a series of oligosaccharides with repeating disaccharide units. The glycoside hydrolase (GH) module of agarase is known to be responsible for its catalytic activity. However, variations in the composition of the GH module and its effects
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