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Identification of competitive antagonists of the P2Y1 receptor.

J L Boyer et al.

Molecular pharmacology, 50(5), 1323-1329 (1996-11-01)

Although P2 receptors mediate a myriad of physiological effects of extracellular adenine nucleotides, study of this broad class of receptors has been compromised by a lack of P2 receptor-selective antagonist molecules. The adenine nucleotide-promoted inositol lipid hydrolysis response of turkey

The P2Y1 receptor antagonist adenosine-2',5'-diphosphate non-selectively antagonizes the platelet P2X1 ion channel.

E Toth-Zsamboki et al.

Thrombosis and haemostasis, 86(5), 1338-1339 (2002-01-31)

The platelet P2 receptors

Platelets, 259-277 (2019)

Purification, cDNA cloning and functional expression of NADPH-cytochrome P450 reductase from Centaurium erythraea cell cultures.

H Schwarz et al.

Plant biology (Stuttgart, Germany), 11(3), 300-306 (2009-05-28)

Solubilised NADPH-cytochrome P450 reductase (CPR) was purified from the microsomal fraction of centaury (Centaurium erythraea) cell cultures by Q-anion exchange chromatography and affinity chromatography on adenosine 2',5'-diphosphate agarose. SDS-PAGE demonstrated the presence of three CPR isoforms with molecular masses of

P2y1 and P2y2 receptor-operated Ca2+ signals in primary cultures of cardiac microvascular endothelial cells.

F Moccia et al.

Microvascular research, 61(3), 240-252 (2001-05-05)

Intracellular Ca2+ signals elicited by nucleotide agonists were investigated in primary cultures of rat cardiac microvascular endothelial cells using the fura-2 technique. UTP increased the intracellular [Ca2+] in 94% of the cells, whereas 2MeSATP was active in 32%. The rank

Characterisation of pea cytosolic glutathione reductase expressed in transgenic tobacco.

R G Stevens et al.

Planta, 211(4), 537-545 (2000-10-13)

Expression in transgenic tobacco (Nicotiana tabacum L.) of a pea (Pisum sativum L.) GOR2 cDNA, encoding an isoform of glutathione reductase (GOR2), resulted in a 3- to 7-fold elevation of total foliar glutathione reductase (GR) activity. The enzyme encoded by

Inexpensive purification of P450 reductase and other proteins using 2',5'-adenosine diphosphate agarose affinity columns.

D Rock et al.

Protein expression and purification, 22(1), 82-83 (2001-06-05)

Two reductases, P450 oxidoreductase and P450Bm-3 reductase, were purified on a 2',5'-adenosine diphosphate solid support. Although the efficiency of these columns is well established, the cost of the resin and the eluting material 2'-adenosine can be prohibitive. Herein we show

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