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Xianwen Lou et al.
Nature communications, 8, 15420-15420 (2017-05-16)
Numerous self-assembling molecules have been synthesized aiming at mimicking both the structural and dynamic properties found in living systems. Here we show the application of hydrogen/deuterium exchange (HDX) mass spectrometry (MS) to unravel the nanoscale organization and the structural dynamics...
Douglas Zippel et al.
Clinical imaging, 39(3), 408-411 (2015-02-15)
We present our use of magnetic resonance (MR) measurement to determine the amount of residual breast tissue (RBT) following total mastectomy with reconstruction. Breast MR images of 45 women who underwent surgery between January and November 2011 were reviewed. The...
Bo Li et al.
Translational vision science & technology, 9(8), 37-37 (2020-08-29)
To investigate whether human donor lenses are capable of exporting reduced glutathione. Human lenses of varying ages were cultured in artificial aqueous humor for 1 hour under hypoxic conditions to mimic the physiologic environment and reduced glutathione (GSH) and oxidized...
Yirui Guo et al.
Biochemistry, 57(11), 1779-1789 (2018-02-20)
Heme-nitric oxide/oxygen binding (H-NOX) proteins are a group of hemoproteins that bind diatomic gas ligands such as nitric oxide (NO) and oxygen (O2). H-NOX proteins typically regulate histidine kinases (HK) located within the same operon. It has been reported that...
M T Price et al.
Neuropharmacology, 34(8), 1069-1079 (1995-08-01)
Activation of metabotropic glutamate receptors (mGluR) by Glu or related mGluR agonists triggers phosphoinositide (PI) hydrolysis, intracellular Ca2+ mobilization and protein kinase C activation. These mGluR agonist-stimulated events are inhibited strongly by 2-amino-3-phosphono-L-propionic acid (L-AP3) and L-aspartate-beta-hydroxamate (L-A beta H)...
Shawn Xiong et al.
Structure (London, England : 1993), 26(8), 1101-1115 (2018-07-10)
The human NDR family kinases control diverse aspects of cell growth, and are regulated through phosphorylation and association with scaffolds such as MOB1. Here, we report the crystal structure of the human NDR1 kinase domain in its non-phosphorylated state, revealing...
Véronique Sauvé et al.
Nature structural & molecular biology, 25(7), 623-630 (2018-07-04)
Mutations in the ubiquitin ligase parkin are responsible for a familial form of Parkinson's disease. Parkin and the PINK1 kinase regulate a quality-control system for mitochondria. PINK1 phosphorylates ubiquitin on the outer membrane of damaged mitochondria, thus leading to recruitment...
Jingjing Pei et al.
Proceedings of the National Academy of Sciences of the United States of America, 118(10) (2021-03-03)
Human respiratory syncytial virus (RSV) nonstructural protein 2 (NS2) inhibits host interferon (IFN) responses stimulated by RSV infection by targeting early steps in the IFN-signaling pathway. But the molecular mechanisms related to how NS2 regulates these processes remain incompletely understood....
Graciela Lorca et al.
Journal of bacteriology, 185(10), 3218-3222 (2003-05-06)
YveA of Bacillus subtilis, a putative transporter of the amino acid/polyamine/organocation (APC) superfamily, is shown to mediate uptake of both L-aspartate and L-glutamate as well as having sensitivity to L-aspartate hydroxamate. This 14 TMS protein is the primary aspartate uptake...
Gerard Quash et al.
Bulletin du cancer, 91(4), E61-E79 (2004-11-26)
We have previously shown that the addition of 4-methylthio-2-oxobutanoate (MTOB) to cultures of methionine dependent neoplastic cells which lack endogenous MTOB restores their capacity to grow in the absence of exogenous methionine. Transition state inhibitors of the MTOB transaminase,responsible for...
J W Deitmer et al.
Glia, 19(2), 111-122 (1997-02-01)
Glutamate is an excitatory receptor agonist in both neurones and glial cells, and, in addition, glutamate is also a substrate for glutamate transporter in glial cells. We have measured intracellular and extracellular pH changes induced by bath application of glutamate...
Svetlana N Rubtsova et al.
PloS one, 10(7), e0133578-e0133578 (2015-07-25)
Using confocal microscopy, we analyzed the behavior of IAR-6-1, IAR1170, and IAR1162 transformed epithelial cells seeded onto the confluent monolayer of normal IAR-2 epithelial cells. Live-cell imaging of neoplastic cells stably expressing EGFP and of normal epithelial cells stably expressing...
David Blocquel et al.
Proceedings of the National Academy of Sciences of the United States of America, 116(39), 19440-19448 (2019-09-11)
Aminoacyl-transfer RNA (tRNA) synthetases (aaRSs) are the largest protein family causatively linked to neurodegenerative Charcot-Marie-Tooth (CMT) disease. Dominant mutations cause the disease, and studies of CMT disease-causing mutant glycyl-tRNA synthetase (GlyRS) and tyrosyl-tRNA synthetase (TyrRS) showed their mutations create neomorphic...
Ana Andres-Hernando et al.
JCI insight, 6(1) (2020-12-16)
Subjects with obesity frequently have elevated serum vasopressin levels, noted by measuring the stable analog, copeptin. Vasopressin acts primarily to reabsorb water via urinary concentration. However, fat is also a source of metabolic water, raising the possibility that vasopressin might...
Claudia Lanvers et al.
Analytical biochemistry, 309(1), 117-126 (2002-10-17)
The enzyme L-asparaginase (ASNASE), which hydrolyzes L-asparagine (L-Asn) to ammonia and L-aspartic acid (L-Asp), is commonly used for remission induction in acute lymphoblastic leukemia. To correlate ASNASE activity with L-Asn reduction in human serum, sensitive methods for the determination of...
Hanliu Wang et al.
Biochemistry, 54(42), 6475-6481 (2015-09-30)
We report the use of hydrogen-deuterium amide exchange coupled to mass spectrometry (HDX-MS) to study the interfaces of and conformational changes accompanying CsgE oligomerization. This protein plays an important role in enteric bacteria biofilm formation. Biofilms provide protection for enteric...
Suzanne G Mays et al.
Molecular pharmacology, 92(1), 1-11 (2017-04-02)
Peroxisome proliferator-activated gamma coactivator 1-
D A Gibson et al.
Human reproduction (Oxford, England), 30(6), 1290-1301 (2015-03-31)
Does intrauterine biosynthesis of estrogen play an important role in early pregnancy by altering the function of uterine natural killer (uNK) cells? Estrogens directly regulate the function of human uNK cells by increasing uNK cell migration and secretion of uNK...
João Carlos Rodrigues et al.
BMJ open, 10(9), e037239-e037239 (2020-09-05)
Although several imaging options are available for diagnosing syndesmotic injury, a fundamental question that guides treatment remains unanswered. Syndesmotic instability is still challenging to diagnose correctly, and syndesmotic disruption and true syndesmotic instability should be differentiated. Currently, imaging tests quickly...
Sandra N Majuta et al.
Journal of the American Society for Mass Spectrometry, 30(6), 1102-1114 (2019-04-14)
Rapid, solution-phase hydrogen/deuterium exchange (HDX) coupled with mass spectrometry (MS) is demonstrated as a means for distinguishing small-molecule metabolites. HDX is achieved using capillary vibrating sharp-edge spray ionization (cVSSI) to allow sufficient time for reagent mixing and exchange in micrometer-sized...
G J Stephens et al.
Receptors & channels, 1(1), 39-52 (1993-01-01)
Electrophysiological effects of the excitatory amino acids (EAAs) glutamate (Glu) and kainate (KA) on membrane properties of confluent (> 7-9 day) astrocyte cultures were examined. The whole-cell patch clamp technique was employed to measure membrane currents. Cells were subdivided antigenically...
Aleksandra S Chikina et al.
Biology of the cell, 111(10), 245-261 (2019-08-14)
Metastatic disease is caused by the ability of cancer cells to reach distant organs and form secondary lesions at new locations. Dissemination of cancer cells depends on their migration plasticity - an ability to switch between motility modes driven by...
Harpreet Kaur et al.
Methods (San Diego, Calif.), 153, 13-21 (2018-11-26)
Colocalization single-molecule methods can provide a wealth of information concerning the ordering and dynamics of biomolecule assembly. These have been used extensively to study the pathways of spliceosome assembly in vitro. Key to these experiments is the measurement of binding...
Richard Y-C Huang et al.
mAbs, 10(1), 95-103 (2017-11-15)
TL1A, a tumor necrosis factor-like cytokine, is a ligand for the death domain receptor DR3. TL1A, upon binding to DR3, can stimulate lymphocytes and trigger secretion of proinflammatory cytokines. Therefore, blockade of TL1A/DR3 interaction may be a potential therapeutic strategy...
Catherine S Giess et al.
AJR. American journal of roentgenology, 208(6), 1378-1385 (2017-03-08)
The purpose of this study was to determine the clinical utility of breast MRI for diagnosing malignancy in women with equivocal mammographic findings but no symptoms. Retrospective review of an institutional MRI database of 7332 contrast-enhanced breast MRI examinations from...
Hillary E Hoffman et al.
Journal of medicinal chemistry, 52(19), 6032-6041 (2009-10-02)
Mammalian serine racemase (SR) is a pyridoxal-5'-phosphate (PLP) dependent enzyme responsible for the biosynthesis of the neurotransmitter D-serine, which activates N-methyl-D-aspartate (NMDA) receptors in the CNS. Aberrant regulation of NMDA receptor signaling has been implicated in a variety of neuropathologies...
Shaoyong Lu et al.
Journal of medicinal chemistry, 62(14), 6405-6421 (2019-03-01)
Allosteric modulators bound to structurally diverse allosteric sites can achieve better pharmacological advantages than orthosteric ligands. The discovery of allosteric modulators, however, has been traditionally serendipitous, owing to the complex nature of allosteric modulation. Recent advances in the understanding of...
Hanliu Wang et al.
International journal of mass spectrometry, 420, 16-23 (2017-10-24)
Bacteria within Curli biofilms are protected from environmental pressures (e.g., disinfectants, antibiotics), and this is responsible for intractable infections. Understanding aggregation of the major protein component of Curli, CsgA, may uncover disease-associated amyloidogenesis mechanisms. Here, we report the application of...
Jana Jirásková-Vaníčková et al.
Current drug targets, 12(7), 1037-1055 (2011-02-05)
Proteins of glutamatergic NMDA receptor signaling pathways have been studied as targets for intervention in a variety of neuropathological conditions, including neurodegenerations, epilepsy, neuropathic pain, drug addiction, and schizophrenia. High activity NMDA-blocking agents have been designed to treat some of...
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