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Hyung-Keun Ku et al.
Analytical biochemistry, 434(1), 178-180 (2012-12-04)
The Bradford assay is a simple method for protein quantitation, but variation in the results between proteins is a matter of concern. In this study, we compared and normalized quantitative values from two models for protein quantitation, where the residues
Clivandir Severino da Silva et al.
Frontiers in behavioral neuroscience, 13, 240-240 (2019-12-05)
Neonatal lipopolysaccharide (LPS) exposure-induced brain inflammation has been associated to neuronal injury and facilitates the development of models of neurological disorders in adult rats. The P2X7 receptor (P2X7R) plays a fundamental role in the onset and maintenance of the inflammatory
Søren U Nielsen et al.
The Journal of general virology, 89(Pt 10), 2507-2517 (2008-09-18)
Hepatitis C virus (HCV) particles found in vivo are heterogeneous in density and size, but their detailed characterization has been restricted by the low titre of HCV in human serum. Previously, our group has found that HCV circulates in blood
Lene K Vestby et al.
BMC veterinary research, 5, 43-43 (2009-11-26)
Biofilm has been shown to be one way for Salmonella to persist in the feed factory environment. Matrix components, such as fimbriae and cellulose, have been suggested to play an important role in the survival of Salmonella in the environment.
Sungwon Han et al.
Analytical and bioanalytical chemistry, 405(6), 1821-1831 (2012-09-25)
Gel electrophoresis is routinely used to separate and analyse macromolecules in biological systems. Although many of these electrophoretic techniques necessitate the denaturing of the analytes prior to their analysis, blue native polyacrylamide gel electrophoresis (BN-PAGE) permits the investigation of proteins/enzymes
Atef M K Nassar et al.
Molecules (Basel, Switzerland), 25(11) (2020-06-11)
Sumithion (Fenitrothion) (SUM) is an organophosphorus insecticide used to combat a wide variety of plant pests. Exposure to SUM causes significant toxicity to the brain, liver, kidney, and reproductive organs through, for example, binding to DNA, and it induces DNA
David E Pelayes et al.
Ophthalmic research, 48 Suppl 1, 21-25 (2012-08-29)
Brilliant blue G (BBG) is frequently used in chromovitrectomy to facilitate internal limiting membrane (ILM) peeling. A study was initiated to evaluate if heavy BBG is safe and effective in staining the ILM. We studied 30 eyes, 23 with idiopathic
Nadine Dyballa et al.
Methods in molecular biology (Clifton, N.J.), 893, 47-59 (2012-06-06)
Proteins separated by two-dimensional gel electrophoresis can be visualized by in-gel detection using -different staining methods. Ideally, the dye should bind non-covalently to the protein following a linear response curve. Since protein concentrations in biological systems may vary by six
Maide Ozen et al.
Pediatric research, 87(3), 463-471 (2019-09-08)
Magnesium sulfate (MgSO4) is utilized for fetal neuroprotection in preterm birth but its mechanism of action is still poorly understood. P2X7 receptor (P2X7R) is required for secretion of IL-1β, and can be blocked by divalent cations such as magnesium (Mg)
Shoji Notomi et al.
PloS one, 8(1), e53338-e53338 (2013-01-12)
Photoreceptor degeneration is the most critical cause of visual impairment in age-related macular degeneration (AMD). In neovascular form of AMD, severe photoreceptor loss develops with subretinal hemorrhage due to choroidal neovascularization (CNV), growth of abnormal blood vessels from choroidal circulation.
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Yüksel Totan et al.
The British journal of ophthalmology, 99(1), 75-80 (2014-08-06)
To evaluate the potential efficacy and safety of inner limiting membrane (ILM) staining with brilliant blue G (BBG) under air infusion. 63 patients, treated by one surgeon with macular hole or idiopathic epiretinal membrane were evaluated prospectively. BBG was applied
Melatonin attenuates hypertension-induced renal injury partially through inhibiting oxidative stress in rats.
Qiao YF, et al.
Molecular Medicine Reports, 13, 21-21 (2016)
Belinda Yau et al.
Scientific reports, 9(1), 19466-19466 (2019-12-21)
A threonine-to-Isoleucine (Thr52Ile) mutation within the pro-domain of the Sorcs1 gene was positionally cloned as the gene underlying a quantitative trait locus that affects fasting insulin levels in mice. In humans, genome-wide association studies and linkage studies have shown that
P2X7 antagonism using Brilliant Blue G reduces body weight loss and prolongs survival in female SOD1G93A amyotrophic lateral sclerosis mice.
PeerJ, e3064-e3064 (2017)
Adam Aguirre et al.
Journal of cellular physiology, 228(2), 485-493 (2012-07-19)
Fas ligation via the ligand FasL activates the caspase-8/caspase-3-dependent extrinsic death pathway. In so-called type II cells, an additional mechanism involving tBid-mediated caspase-9 activation is required to efficiently trigger cell death. Other pathways linking FasL-Fas interaction to activation of the
Song Wei et al.
Bioorganicheskaia khimiia, 38(5), 535-544 (2013-01-25)
A novel 4-(tetrahydro-2-furanmethoxy)-N-octadecyl-1,8-naphthalimide (4-TN1) blue emitting fluorophore was synthesized for solvent polarity probing and protein detection. The effect of various solvents on absorption and fluorescence spectra of 4-TNI was investigated. By comparison with other derivatives with heterocyclic electron-donating groups, 4-TNI
Liyen Katrina Kan et al.
Purinergic signalling, 16(3), 327-336 (2020-06-26)
Gliomas, the most common primary brain cancer, are highly infiltrative and extremely difficult to treat. Despite advancements, current treatment is limited, with patients surviving for a median of 14-15 months post-diagnosis. Previous research has demonstrated the upregulation of a purinergic receptor
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Sheng Chen et al.
Critical care medicine, 41(12), e466-e474 (2013-08-22)
Brilliant blue G, a selective P2X7 receptor antagonist, exhibits neuroprotective properties. This study examined whether brilliant blue G treatment ameliorates early brain injury after experimental subarachnoid hemorrhage, specifically via inhibiting p38 mitogen-activated protein kinase-related proapoptotic pathways. Controlled in vivo laboratory
Veronica Zaga-Clavellina et al.
Reproductive biology and endocrinology : RB&E, 9, 13-13 (2011-01-27)
Premature rupture of fetal membranes (PROM) complicated with intrauterine infection has been associated to alterations of the extracellular matrix (ECM) homeostasis. The aim of this work was to evaluate the integral/functional response of the amnion (AMN) and choriodecidua (CHD) to
Rahul Bhattacharjee et al.
Molecular biology of the cell, 31(9), 917-929 (2020-02-27)
In many organisms, positive and negative signals cooperate to position the division site for cytokinesis. In the rod-shaped fission yeast Schizosaccharomyces pombe, symmetric division is achieved through anillin/Mid1-dependent positive cues released from the central nucleus and negative signals from the
Joseph M Tuscano et al.
Cancer immunology, immunotherapy : CII, 60(6), 771-780 (2011-02-25)
Previous studies have shown that bispecific antibodies that target both CD20 and CD22 have in vivo lymphomacidal properties. We developed a CD20-CD22 bispecific antibody (Bs20x22) from anti-CD20 and the anti-CD22 monoclonal antibodies (mAb), rituximab and HB22.7, respectively. Bs20x22 was constructed
Jijun Zhao et al.
Arthritis and rheumatism, 65(12), 3176-3185 (2013-09-12)
The NLRP3 inflammasome plays key roles in inflammation and autoimmunity, and purinergic receptor P2X7 has been proposed to be upstream of NLRP3 activation. The aim of the present study, using murine models, was to investigate whether the P2X7 /NLRP3 inflammasome
COMPARATIVE EVALUATION OF SOMATIC PROTEINS OF THE NYMPHAL STAGE OF LINGUATULA SERRATA ISOLATED FROM GOATS AND CATTLE IN NORTHWEST IRAN.
Bulgarian Journal of Veterinary Medicine, 19, 242-246 (2016)
Y L Ooi et al.
Eye (London, England), 27(3), 425-431 (2012-12-01)
To compare the potential retinal toxicity of two commercially Brilliant blue G dyes (Brilliant Peel and Ocublue Plus) and Indocyanine green (ICG) at usual clinical concentration. Brilliant Peel 0.025% (n=9), Ocublue Plus 0.025% (n=9), and ICG 0.05% (n=9) were injected
Wei Zhang et al.
Frontiers in cellular neuroscience, 13, 152-152 (2019-05-10)
Morphological and functional polarization of neurons depends on the generation and maintenance of the axon initial segment (AIS). This axonal domain maintains axonal properties but is also the place where the action potential (AP) is generated. All these functions require
Chiara Rigato et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 32(34), 11559-11573 (2012-08-24)
Microglia are known to invade the mammalian spinal cord (SC) at an early embryonic stage. While the mechanisms underlying this early colonization of the nervous system are still unknown, we recently found that it is associated, at least partially, with
Human cells adapt to translational errors by modulating protein synthesis rate and protein turnover.
Ana Sofia Varanda et al.
RNA biology, 17(1), 135-149 (2019-10-02)
Deregulation of tRNAs, aminoacyl-tRNA synthetases (aaRS) or tRNA modifying enzymes, increase the level of protein synthesis errors (PSE) and are associated with several diseases, but the cause-effect mechanisms of these pathologies remain elusive. To clarify the role of PSE in
Kristen L Huber et al.
Protein expression and purification, 67(2), 139-147 (2009-05-22)
Peptide libraries have proven to be useful in applications such as substrate profiling, drug candidate screening and identifying protein-protein interaction partners. However, issues of fidelity, peptide length, and purity have been encountered when peptide libraries are chemically synthesized. Biochemically produced
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