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Jeff G Wilkesman et al.
Electrophoresis, 28(3), 429-436 (2006-12-30)
Proteolytic activities isolated from the marine demosponges Geodia cydonium and Suberites domuncula were analyzed by 2-D zymography, a technique that combines IEF and zymography. After purification, a 200 kDa proteolytically active protein band was obtained from G. cydonium when analyzed
Panagiota G Stathopoulou et al.
BMC microbiology, 9, 107-107 (2009-05-29)
The oral pathogen Porphyromonas gingivalis has been shown to modulate apoptosis in different cell types, but its effect on epithelial cells remains unclear. We demonstrate that primary human gingival epithelial cells (HGECs) challenged with live P. gingivalis for 24 hours
Leticia Reyes et al.
PloS one, 8(9), e74230-e74230 (2013-09-27)
P. gingivalis (Pg), a causative agent of chronic generalized periodontitis, has been implicated in promoting cardiovascular disease. Expression of lipoprotein gene PG0717 of Pg strain W83 was found to be transiently upregulated during invasion of human coronary artery endothelial cells
Benjaporn Treetharnmathurot et al.
International journal of pharmaceutics, 357(1-2), 252-259 (2008-03-01)
PEGylated proteins are routinely used as therapeutics, but systematic studies of the effect of PEG molecular weight and linking chemistry on the biological activity and particularly the thermal stability of the conjugated protein are rarely made. Here, activated monomethoxypolyethylene glycol
Yunfeng Zhang et al.
Bioresource technology, 247, 81-87 (2017-09-26)
Streptomyces griseus trypsin (SGT) possesses enzymatic properties similar to mammalian trypsins and has great potential applications in the leather processing, bioethanol, detergent and pharmaceutical industry. Here, a new strategy was reported for improving its stable, active secretory production through engineering
Maria Soledad Michiels et al.
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 204, 228-235 (2016-12-08)
No studies are available about biochemical characteristics and modulation (i.e. by endogenous and/or environmental cues) of trypsin (a key digestive endoprotease) in hepatopancreas of intertidal euryhaline crabs neither on the possible concomitant modulation of key ectoproteases such as aminopeptidase-N (APN)
Naresh Arora et al.
PloS one, 8(6), e66603-e66603 (2013-07-05)
Strains of Bacillus thuringiensis produce insecticidal proteins. These strains have been isolated from diverse ecological niches, such as soil, phylloplane, insect cadavers and grain dust. To effectively propagate, these strains produce a range of molecules that facilitate its multiplication in
Tatiana A Valueva et al.
Journal of pathogens, 2011, 947218-947218 (2011-01-01)
Proteinases secreted by the oomycete Phytophthora infestans (Mont.) de Bary, Rhizoctonia solani, and Fusarium culmorum belonging to different families of fungi have been studied to determine if the exoenzyme secretion depends on the environmental conditions and the phylogenetic position of
Jakub Zdarta et al.
Colloids and surfaces. B, Biointerfaces, 169, 118-125 (2018-05-15)
In the presented study synthesized magnetic nanoparticles were used as an inorganic precursor for the preparation of novel magnetite-lignin and magnetite-chitin hybrid supports for enzyme immobilization. Effective synthesis of the hybrids was confirmed by Fourier transform infrared spectroscopy and powder
Julia Stolarow et al.
BMC biotechnology, 15, 77-77 (2015-08-20)
Immobilization of enzymes onto different carriers increases enzyme's stability and reusability within biotechnological and pharmaceutical applications. However, some immobilization techniques are associated with loss of enzymatic specificity and/or activity. Possible reasons for this loss are mass transport limitations or structural
Monika Rani et al.
Journal of food science and technology, 56(3), 1192-1201 (2019-04-09)
Idli is a naturally fermented food, prepared using dehulled black gram dal and parboiled rice. In the first phase of the study, replacement of rice with some underutilized cereals like amaranth and finger millet was carried out at different proportions
B Oppert et al.
Bulletin of entomological research, 96(2), 167-172 (2006-03-25)
Digestion in the larger black flour beetle, Cynaeus angustus (LeConte), was studied to identify new control methods for this pest of stored grains and grain products. The physiological pH of the larval gut, as measured with extracts in water, was
Digestive enzymes and stylet morphology of Deraeocoris nigritulus (Uhler)(Hemiptera: Miridae) reflect adaptations for predatory habits
Boyd DW
Annals of the Entomological Society of America, 96(5), 667-671 (2014)
Yubo Hou et al.
In vitro cellular & developmental biology. Animal, 53(7), 593-603 (2017-06-22)
Gingipains secreted by Porphyromonas gingivalis (P. gingivalis, Pg) play an important role in maintaining macrophage infiltrating. And, this study is to evaluate effects of gingipain on M1 macrophage polarization after exposure to Porphyromonas gingivalis (P. gingivalis, Pg) and if these
Shanshan Li et al.
Molecules (Basel, Switzerland), 18(1), 1138-1149 (2013-01-18)
The enzymatic performance of trypsin in hydrolysis of N-α-benzoyl-DL-arginine-4-nitroanilide (BAPNA) was improved by adsorption on Santa Barbara Amorphous (SBA)-15 mesoporous silica. The optimal immobilization conditions were screened and the properties of immobilized enzyme have also been studied. Under the optimal
Ezequiel R Coscueta et al.
Food chemistry, 198, 36-44 (2016-01-16)
Enzymatic hydrolysis of soybean meal protein isolate (SPI) obtained under two temperature conditions with Corolase PP was studied, assessing the impact of hydrolysis on potential antioxidant and antihypertensive activities. The protein was isolated from soybean meal under controlled conditions of
S Shakilanishi et al.
International journal of biological macromolecules, 103, 1069-1076 (2017-05-31)
Enzyme based dehairing of animal skins in leather processing is considered a greener alternative to conventional chemical based dehairing process. The specificity based screening of proteases would be an important criterion as it would help in removing hairs by acting
Giovanni Covaleda et al.
Journal of proteomics, 165, 75-92 (2017-06-13)
Proteases and their inhibitors have become molecules of increasing fundamental and applicative value. Here we report an integrated strategy to identify and analyze such inhibitors from Caribbean marine invertebrates extracts by a fast and sensitive functional proteomics-like approach. The strategy
M Carmen Arques et al.
Journal of agricultural and food chemistry, 64(8), 1823-1830 (2016-02-16)
Soy milk is a health-promoting beverage of which consumption is steadily expanding. Different bioactivities have been associated with soy products such as antioxidant capacity, anti-inflammatory properties, or decrease of cancer development risk. These activities have been related to the presence
Atiyeh Mahdavi et al.
Journal of insect science (Online), 13, 81-81 (2013-11-16)
Proteolytic activities in digestive system extracts from the larval midgut of the lesser mulberry pyralid, Glyphodes pyloalis Walker (Lepidoptera: Pyralidae), were analyzed using different specific peptide substrates and proteinase inhibitors. High proteolytic activities were found at pH 10.0 and a
Vinod Kumar et al.
Archives of biochemistry and biophysics, 566, 15-25 (2014-12-21)
Legume Bowman-Birk inhibitors (BBIs) that inhibit mammalian proteases exist as dimers in solution. The structural basis governing dimerization of HGI-III (horsegram seed BBI) was investigated. An intra-monomer salt bridge (D76-K71) stabilizes an atypical hook-like conformation at the C-terminus. We postulate
Japjot Sekhon et al.
Journal of food science and technology, 54(11), 3598-3611 (2017-10-21)
Three released cultivars, forty four advance breeding lines and three wild species of pigeonpea (
Roall Espersen et al.
Applied microbiology and biotechnology, 104(6), 2513-2522 (2020-01-29)
Two proteases, named C- and T-like proteases, respectively, were purified from the culture supernatant of Amycolatopsis keratinophila subsp. keratinophila D2T grown on a keratinous slaughterhouse by-product of pig bristles and nails as sole nitrogen and carbon source. The two proteases
Tooba Siddiqui et al.
Journal of fluorescence, 29(4), 969-979 (2019-07-22)
Gallic acid is a naturally occurring plant polyphenol found in green tea and various fruits. Under certain conditions gallic acid exhibits pro-oxidant characteristics rather than its well known antioxidant property. In the present work, we explored the interaction of gallic
Ana C Figueiredo et al.
PloS one, 7(3), e34354-e34354 (2012-03-30)
The tremendous social and economic impact of thrombotic disorders, together with the considerable risks associated to the currently available therapies, prompt for the development of more efficient and safer anticoagulants. Novel peptide-based thrombin inhibitors were identified using in silico structure-based
Xiang Wang et al.
FEBS letters, 594(15), 2462-2471 (2020-05-18)
MS5 is a meiosis-related protein belonging to the Brassicaceae-specific domain of unknown function family and characterized by the MS5 superfamily domain (MSD). In this study, we elucidated the three-dimensional crystal structure and potential biochemical function of the MSD. It was
Yuanhuai Peng et al.
Food chemistry, 290, 277-285 (2019-04-20)
Fluorescein-isothiocyanate (FITC) labeled trypsin-like protease was prepared and injected into the hepatopancreas of white shrimp. Different segments of the injected shrimp were analyzed with a fluorescence microscope during storage. FITC-trypsin-like protease can be detected in the first segment of shrimp
Aline Medeiros Lima et al.
Planta, 252(2), 16-16 (2020-07-15)
A new Piper nigrum cysteine proteinase inhibitor, PnCPI, belonging to group I of phytocystatins, with inhibitory activity against papain and growth of Fusarium solani f. sp. piperis, was isolated and characterized. Previous studies (de Souza et al. 2011) have identified
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