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Hyung-Keun Ku et al.
Analytical biochemistry, 434(1), 178-180 (2012-12-04)
The Bradford assay is a simple method for protein quantitation, but variation in the results between proteins is a matter of concern. In this study, we compared and normalized quantitative values from two models for protein quantitation, where the residues
Søren U Nielsen et al.
The Journal of general virology, 89(Pt 10), 2507-2517 (2008-09-18)
Hepatitis C virus (HCV) particles found in vivo are heterogeneous in density and size, but their detailed characterization has been restricted by the low titre of HCV in human serum. Previously, our group has found that HCV circulates in blood
Lene K Vestby et al.
BMC veterinary research, 5, 43-43 (2009-11-26)
Biofilm has been shown to be one way for Salmonella to persist in the feed factory environment. Matrix components, such as fimbriae and cellulose, have been suggested to play an important role in the survival of Salmonella in the environment.
Sungwon Han et al.
Analytical and bioanalytical chemistry, 405(6), 1821-1831 (2012-09-25)
Gel electrophoresis is routinely used to separate and analyse macromolecules in biological systems. Although many of these electrophoretic techniques necessitate the denaturing of the analytes prior to their analysis, blue native polyacrylamide gel electrophoresis (BN-PAGE) permits the investigation of proteins/enzymes
David E Pelayes et al.
Ophthalmic research, 48 Suppl 1, 21-25 (2012-08-29)
Brilliant blue G (BBG) is frequently used in chromovitrectomy to facilitate internal limiting membrane (ILM) peeling. A study was initiated to evaluate if heavy BBG is safe and effective in staining the ILM. We studied 30 eyes, 23 with idiopathic
Nadine Dyballa et al.
Methods in molecular biology (Clifton, N.J.), 893, 47-59 (2012-06-06)
Proteins separated by two-dimensional gel electrophoresis can be visualized by in-gel detection using -different staining methods. Ideally, the dye should bind non-covalently to the protein following a linear response curve. Since protein concentrations in biological systems may vary by six
Francisca Díaz et al.
Current protocols in human genetics, Chapter 19, Unit19-Unit19 (2009-10-07)
The oxidative phosphorylation (OXPHOS) system consists of five multimeric complexes embedded in the mitochondrial inner membrane. They work in concert to drive the aerobic synthesis of ATP. Mitochondrial and nuclear DNA mutations affecting the accumulation and function of these enzymes
Shoji Notomi et al.
PloS one, 8(1), e53338-e53338 (2013-01-12)
Photoreceptor degeneration is the most critical cause of visual impairment in age-related macular degeneration (AMD). In neovascular form of AMD, severe photoreceptor loss develops with subretinal hemorrhage due to choroidal neovascularization (CNV), growth of abnormal blood vessels from choroidal circulation.
Yüksel Totan et al.
The British journal of ophthalmology, 99(1), 75-80 (2014-08-06)
To evaluate the potential efficacy and safety of inner limiting membrane (ILM) staining with brilliant blue G (BBG) under air infusion. 63 patients, treated by one surgeon with macular hole or idiopathic epiretinal membrane were evaluated prospectively. BBG was applied
Adam Aguirre et al.
Journal of cellular physiology, 228(2), 485-493 (2012-07-19)
Fas ligation via the ligand FasL activates the caspase-8/caspase-3-dependent extrinsic death pathway. In so-called type II cells, an additional mechanism involving tBid-mediated caspase-9 activation is required to efficiently trigger cell death. Other pathways linking FasL-Fas interaction to activation of the
Song Wei et al.
Bioorganicheskaia khimiia, 38(5), 535-544 (2013-01-25)
A novel 4-(tetrahydro-2-furanmethoxy)-N-octadecyl-1,8-naphthalimide (4-TN1) blue emitting fluorophore was synthesized for solvent polarity probing and protein detection. The effect of various solvents on absorption and fluorescence spectra of 4-TNI was investigated. By comparison with other derivatives with heterocyclic electron-donating groups, 4-TNI
Sheng Chen et al.
Critical care medicine, 41(12), e466-e474 (2013-08-22)
Brilliant blue G, a selective P2X7 receptor antagonist, exhibits neuroprotective properties. This study examined whether brilliant blue G treatment ameliorates early brain injury after experimental subarachnoid hemorrhage, specifically via inhibiting p38 mitogen-activated protein kinase-related proapoptotic pathways. Controlled in vivo laboratory
Veronica Zaga-Clavellina et al.
Reproductive biology and endocrinology : RB&E, 9, 13-13 (2011-01-27)
Premature rupture of fetal membranes (PROM) complicated with intrauterine infection has been associated to alterations of the extracellular matrix (ECM) homeostasis. The aim of this work was to evaluate the integral/functional response of the amnion (AMN) and choriodecidua (CHD) to
Joseph M Tuscano et al.
Cancer immunology, immunotherapy : CII, 60(6), 771-780 (2011-02-25)
Previous studies have shown that bispecific antibodies that target both CD20 and CD22 have in vivo lymphomacidal properties. We developed a CD20-CD22 bispecific antibody (Bs20x22) from anti-CD20 and the anti-CD22 monoclonal antibodies (mAb), rituximab and HB22.7, respectively. Bs20x22 was constructed
Jijun Zhao et al.
Arthritis and rheumatism, 65(12), 3176-3185 (2013-09-12)
The NLRP3 inflammasome plays key roles in inflammation and autoimmunity, and purinergic receptor P2X7 has been proposed to be upstream of NLRP3 activation. The aim of the present study, using murine models, was to investigate whether the P2X7 /NLRP3 inflammasome
Y L Ooi et al.
Eye (London, England), 27(3), 425-431 (2012-12-01)
To compare the potential retinal toxicity of two commercially Brilliant blue G dyes (Brilliant Peel and Ocublue Plus) and Indocyanine green (ICG) at usual clinical concentration. Brilliant Peel 0.025% (n=9), Ocublue Plus 0.025% (n=9), and ICG 0.05% (n=9) were injected
Chiara Rigato et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 32(34), 11559-11573 (2012-08-24)
Microglia are known to invade the mammalian spinal cord (SC) at an early embryonic stage. While the mechanisms underlying this early colonization of the nervous system are still unknown, we recently found that it is associated, at least partially, with
Broc R Wenrich et al.
Analytical biochemistry, 428(2), 93-95 (2012-06-30)
The Bradford assay has been used reliably for decades to quantify protein in solution. The analyte is incubated in acidic solution of Coomassie Blue G-250 dye, during which reversible ionic and nonionic binding interactions form. Bradford assay color yields were
Kristen L Huber et al.
Protein expression and purification, 67(2), 139-147 (2009-05-22)
Peptide libraries have proven to be useful in applications such as substrate profiling, drug candidate screening and identifying protein-protein interaction partners. However, issues of fidelity, peptide length, and purity have been encountered when peptide libraries are chemically synthesized. Biochemically produced
Kei Takayama et al.
Investigative ophthalmology & visual science, 53(11), 7389-7394 (2012-10-04)
We compared the phototoxicity of indocyanine green (ICG) and Brilliant Blue G (BBG) in cultured RPE cells under fluorescent lamp illumination imitating ambient light. Cultured human RPE line cells were stained with ICG or BBG solution at concentrations of clinical
Felipe P P Almeida et al.
JAMA ophthalmology, 133(1), 85-88 (2014-10-17)
This case report describes a man who developed retinal changes in his right eye associated with brilliant blue G migration into the subretinal space during 2 years of follow-up. The patient's best-corrected visual acuity in the right eye was 20/70
Paul B Henrich et al.
Acta ophthalmologica, 91(2), e120-e124 (2012-11-21)
To evaluate the potential of heavier-than-water brilliant blue G (BBG-D(2) 0) to stain the internal limiting membrane (ILM) during chromovitrectomy. In a nonrandomized, prospective, clinical multicentre study, 71 consecutive chromovitrectomy interventions in 71 patients were analysed. During routine 23-gauge vitrectomy
Bernd Thiede et al.
Molecular & cellular proteomics : MCP, 12(2), 529-538 (2012-10-04)
The proteomics field has shifted over recent years from two-dimensional gel electrophoresis (2-DE)-based approaches to SDS-PAGE or gel-free workflows because of the tremendous developments in isotopic labeling techniques, nano-liquid chromatography, and high-resolution mass spectrometry. However, 2-DE still offers the highest
V S Gasparov et al.
Biokhimiia (Moscow, Russia), 59(6), 763-777 (1994-06-01)
Possible applications of the Coomassie brilliant blue G-250 dye method for protein assay are reviewed. The physico-chemical and optical properties of the dye are considered in detail. The data on the protein-binding mechanism are generalized. Modifications of the Coomassie blue
Hiroshi Enaida et al.
Retina (Philadelphia, Pa.), 33(10), 2170-2174 (2013-04-26)
To measure the concentration of brilliant blue G (BBG) in vitreous and plasma after use as a surgical adjuvant for staining and peeling of the internal limiting membrane to determine potential systemic adverse effects. This study was designed as a
H Edward Wong et al.
ACS chemical neuroscience, 2(11), 645-657 (2012-08-04)
Growing evidence suggests that on-pathway amyloid-β (Aβ) oligomers are primary neurotoxic species and have a direct correlation with the onset of Alzheimer's disease (AD). One promising therapeutic strategy to block AD progression is to reduce the levels of these neurotoxic
Timothy Me Scales et al.
Molecular neurodegeneration, 6, 12-12 (2011-01-29)
Tau protein is the principal component of the neurofibrillary tangles found in Alzheimer's disease, where it is hyperphosphorylated on serine and threonine residues, and recently phosphotyrosine has been demonstrated. The Src-family kinase Fyn has been linked circumstantially to the pathology
Yoshifumi Iwamaru et al.
PloS one, 7(5), e37896-e37896 (2012-06-14)
Prion diseases are fatal neurodegenerative disorders with no effective therapy currently available. Accumulating evidence has implicated over-activation of P2X7 ionotropic purinergic receptor (P2X7R) in the progression of neuronal loss in several neurodegenerative diseases. This has led to the speculation that
Linda F McElhiney
International journal of pharmaceutical compounding, 16(3), 190-195 (2012-10-12)
Vital dyes have been used for over a hundred years to assist ophthalmic practitioners in diagnosing their patients' problems. Patients don't always tolerate the commercial products, usually due to the preservatives, and may need a compounded preparation. Some dyes are
Nicole Sessler et al.
Amino acids, 43(3), 1119-1129 (2012-07-24)
Detailed knowledge of the composition of protein complexes is crucial for the understanding of their structure and function; however, appropriate techniques for compositional analyses of complexes largely rely on elaborate tagging, immunoprecipitation, cross-linking and purification strategies. The proteasome is a
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