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Bhavna V Mohite et al.
Applied biochemistry and biotechnology, 169(5), 1497-1511 (2013-01-16)
Bacterial cellulose (BC), a biopolymer, due to its unique properties is valuable for production of vital products in food, textile, medicine, and agriculture. In the present study, the optimal fermentation conditions for enhanced BC production by Gluconacetobacter hansenii NCIM 2529
Lilith M Caballero Aguilar et al.
Journal of colloid and interface science, 587, 240-251 (2020-12-29)
Drug delivery systems such as microspheres have shown potential in releasing biologicals effectively for tissue engineering applications. Microfluidic systems are especially attractive for generating microspheres as they produce microspheres of controlled-size and in low volumes, using micro-emulsion processes. However, the
Siti Shahara Zulfakar et al.
Food microbiology, 34(2), 369-375 (2013-04-02)
Microbial contamination of carcass surfaces occurs during slaughter and post-slaughter processing steps, therefore interventions are needed to enhance meat safety and quality. Although many studies have been done at the macro-level, little is known about specific processes that influence bacterial
Manveen K Sethi et al.
Molecular omics, 16(4), 364-376 (2020-04-21)
Advancement in mass spectrometry has revolutionized the field of proteomics. However, there remains a gap in the analysis of protein post-translational modifications (PTMs), particularly for glycosylation. Glycosylation, the most common form of PTM, is involved in most biological processes; thus
Yohan Yoon et al.
Meat science, 95(2), 317-322 (2013-06-12)
This study evaluated chemical tenderizers and cooking methods to inactivate Escherichia coli O157:H7 in ground beef patties (model system for non-intact beef). Ground beef was inoculated with E. coli O157:H7 and mixed with (i) nothing (control), (ii) calcium chloride (CC)
Avinash A Kadam et al.
Bioresource technology, 132, 276-284 (2013-02-16)
Pretreatments to sugarcane bagasse (SCB) such as CaCl2, alkali, ammonia, steam and milling showed 91%, 46%, 47%, 42% and 56% adsorption of Solvent Red 5B (SR5B); 92%, 57%, 58%, 56% and 68% adsorption of simulated dyes mixture (SDM), and 86%
Katharina Zimmermann et al.
Nature protocols, 4(2), 174-196 (2009-01-31)
This protocol details methods to identify and record from cutaneous primary afferent axons in an isolated mammalian skin-saphenous nerve preparation. The method is based on extracellular recordings of propagated action potentials from single-fiber receptive fields. Cutaneous nerve endings show graded
Tatiana Jofra et al.
Bio-protocol, 8(5), e2743-e2743 (2018-03-05)
Type 1 diabetes (T1D) is an autoimmune disease caused by the lack of insulin-producing pancreatic beta cells leading to systemic hyperglycemia. Pancreatic islet transplantation is a valid therapeutic approach to restore insulin loss and to promote adequate glycemic control. Pancreatic
Stefanos Stagkourakis et al.
Cell, 182(4), 960-975 (2020-08-09)
Parental behavior is pervasive throughout the animal kingdom and essential for species survival. However, the relative contribution of the father to offspring care differs markedly across animals, even between related species. The mechanisms that organize and control paternal behavior remain
Losee L Ling et al.
Nature, 517(7535), 455-459 (2015-01-07)
Antibiotic resistance is spreading faster than the introduction of new compounds into clinical practice, causing a public health crisis. Most antibiotics were produced by screening soil microorganisms, but this limited resource of cultivable bacteria was overmined by the 1960s. Synthetic
[Ammonium chloride and the calcium chloride loading test for the diagnosis of renal tubular acidosis].
S Takahashi et al.
Nihon rinsho. Japanese journal of clinical medicine, 43(9), 1836-1842 (1985-09-01)
Ekaterina Eremenko et al.
STAR protocols, 2(3), 100643-100643 (2021-07-27)
The organization of chromatin structure plays a crucial role in gene expression, DNA replication, and repair. Chromatin alterations influence gene expression, and modifications could be associated with genomic instability in the cells during aging or diseases. Here, we provide a
Pelin Onsekizoglu Bagci et al.
Journal of food science, 80(9), N2071-N2078 (2015-08-05)
Gold nanoparticles (AuNPs) were synthesized at room temperature following a simple, rapid, and green route using fresh-squeezed apple juice as a reducing reagent. The optimal AuNPs, based on the particle color, stability, and color change suitable for colorimetric detection of
Dhanya Jaikumar et al.
International journal of biological macromolecules, 74, 318-326 (2014-12-30)
Injectable, biodegradable scaffolds are required for soft tissue reconstruction owing to its minimally invasive approach. Such a scaffold can mimic the native extracellular matrix (ECM), provide uniform distribution of cells and overcome limitations like donor site morbidity, volume loss, etc.
Koji Nobe et al.
The Journal of pharmacology and experimental therapeutics, 345(1), 62-68 (2013-02-01)
Adiponectin is an adipose tissue-secreted protein and is a multifunctional adipocytokine. However, the association of adiponectin with bladder contraction has not been investigated. In this study, the adiponectin-sense transgenic mouse (Adip-Sen mouse; age, 16-24 weeks; male) and age-matched controls (C57Bl
Stephanie Rey et al.
Cell reports, 30(6), 2006-2017 (2020-02-13)
Vesicle pool properties are known determinants of synaptic efficacy, but their potential role as modifiable substrates in forms of Hebbian plasticity is still unclear. Here, we investigate this using a nanoscale readout of functionally recycled vesicles in natively wired hippocampal
Aya Watanabe et al.
Clinical science (London, England : 1979), 126(9), 671-678 (2013-11-22)
In the present study we sought to determine the effect of CoCl2, an inhibitor of PHD (prolyl hydroxylase domain protein), on the development of AAA (abdominal aortic aneurysm). AAA was induced in C57BL/6 mice by periaortic application of CaCl2 (AAA
Rita L Strack et al.
Nature protocols, 9(1), 146-155 (2013-12-21)
Genetically encoded fluorescent sensors can be valuable tools for studying the abundance and flux of molecules in living cells. We recently developed a novel class of sensors composed of RNAs that can be used to detect diverse small molecules and
Diana Baigts Allende et al.
Journal of agricultural and food chemistry, 63(31), 7058-7065 (2015-07-15)
To better tailor gelatins for textural characteristics in (food) gels, their interactions are destabilized by introduction of electrostatic repulsions and creation of affinity sites for calcium to "lock" intermolecular interactions. For that purpose gelatins with various degrees of succinylation are
Agnes Schröder et al.
JCI insight, 4(23) (2019-12-06)
Dietary salt consumption leads to cutaneous Na+ storage and is associated with various disorders, including osteopenia. Here, we explore the impact of Na+ and the osmoprotective transcription factor nuclear factor of activated T cell 5 (NFAT5) on bone density and
Norris I Hollie et al.
Biochimica et biophysica acta, 1841(6), 888-895 (2013-12-10)
Mice deficient in group 1b phospholipase A2 have decreased plasma lysophosphatidylcholine and increased hepatic oxidation that is inhibited by intraperitoneal lysophosphatidylcholine injection. This study sought to identify a mechanism for lysophosphatidylcholine-mediated inhibition of hepatic oxidative function. Results showed that in
Marvin D Berman et al.
American journal of physiology. Gastrointestinal and liver physiology, 308(1), G42-G55 (2014-11-02)
Metastable and equilibrium phase diagrams for unconjugated bilirubin IXα (UCB) in bile are yet to be determined for understanding the physical chemistry of pigment gallstone formation. Also, UCB is a molecule of considerable biomedical importance because it is a potent
Eric M Rosenberg et al.
Cell chemical biology, 26(5), 662-673 (2019-03-05)
The G protein-coupled receptor (GPCR) CXCR4 is a co-receptor for HIV and is involved in cancers and autoimmune diseases. We characterized five purine or quinazoline core polyamine pharmacophores used for targeting CXCR4 dysregulation in diseases. All were neutral antagonists for
Juanjuan Zhang et al.
Journal of environmental sciences (China), 25(9), 1719-1725 (2014-02-14)
The development of low-cost and efficient new mineral adsorbents has been a hot topic in recent years. In this study, Friedel's salt (FS: 3CaO x A12O3 x CaCl2 x10H2O), a hexagonal layered inorganic absorbent, was synthesized to remove Cd2+ from
Anita Leocadio Freitas-Mesquita et al.
Experimental parasitology, 137, 8-13 (2013-12-10)
Several ecto-enzymatic activities have been described in the plasma membrane of the protozoan Leishmania amazonensis, which is the major etiological agent of diffuse cutaneous leishmaniasis in South America. These enzymes, including ecto-phosphatases, contribute to the survival of the parasite by
P A Léon Bouyou et al.
Analytical and bioanalytical chemistry, 406(20), 5019-5030 (2014-06-11)
Trace levels of the veterinary antibiotic compound sulfadiazine (SDZ) can be determined in agricultural drainage water samples with this new method. Optimized sample pretreatment and solid-phase extraction was combined with liquid chromatography coupled to tandem mass spectrometry (SPE LC-MS/MS) using
Joseph T Morott et al.
Journal of pharmaceutical sciences, 104(1), 124-134 (2014-11-21)
The primary aim of this research was to produce successfully taste masked formulations of Sildenafil Citrate (SC) using hot-melt extrusion (HME) technology. Multiple screw configurations and polymeric carriers were evaluated for their effects on taste masking efficiency, which was assessed
Evelien Wynendaele et al.
PloS one, 10(11), e0142071-e0142071 (2015-11-05)
Bacteria communicate with each other by the use of signaling molecules, a process called 'quorum sensing'. One group of quorum sensing molecules includes the oligopeptides, which are mainly produced by Gram-positive bacteria. Recently, these quorum sensing peptides were found to
Tieyuan Zhang et al.
Environmental science & technology, 49(5), 2969-2976 (2015-02-03)
Elevated concentration of naturally occurring radioactive material (NORM) in wastewater generated from Marcellus Shale gas extraction is of great concern due to potential environmental and public health impacts. Development of a rapid and robust method for analysis of Ra-226, which
Changyang Linghu et al.
Cell, 183(6), 1682-1698 (2020-11-25)
In order to analyze how a signal transduction network converts cellular inputs into cellular outputs, ideally one would measure the dynamics of many signals within the network simultaneously. We found that, by fusing a fluorescent reporter to a pair of
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