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Lourdes Reyes-Escogido et al.
Antonie van Leeuwenhoek, 98(4), 465-474 (2010-06-18)
We established a Chelex 100-Microwave method for the purification of bacterial genomic DNA (gDNA) in less than 20 min with high yield and good quality, useful for multiple purposes. It combines Chelex 100, proteinase K, RNase A and heating in a
João Pedro Amorim et al.
Cell reports, 32(1), 107862-107862 (2020-07-09)
The notochord is an evolutionary novelty in vertebrates that functions as an important signaling center during development. Notochord ablation in chicken has demonstrated that it is crucial for pancreas development; however, the molecular mechanism has not been fully described. Here
Jared G Panther et al.
Environmental science & technology, 46(4), 2267-2275 (2012-01-25)
Aluminum is acutely toxic, and elevated concentrations of dissolved Al can have detrimental effects on both terrestrial and aquatic ecosystems. Robust analytical methods that can determine environmentally relevant Al fractions accurately and efficiently are required by the environmental monitoring community.
Jackson K Kiptoo et al.
Journal of hazardous materials, 172(2-3), 1163-1167 (2009-08-28)
A solid-phase extraction procedure using mini-columns packed with Chelex-100 and two new chelating agents based on poly(vinyl chloride) functionalized with 3-ferrocenyl-3-hydroxydithioacrylic acid and N,N'-[1,1'-dithiobis(ethylene)]-bis(salicylideneimine) (H(2)sales) loaded on microcrystalline naphthalene, is reported. The columns were used to separate labile copper fractions
Hua-Jie Ba et al.
Fa yi xue za zhi, 23(5), 347-348 (2008-01-08)
To improve DNA extraction from bloodstain on the filter paper and to establish a rapid, simple, and cost-effective method for DNA extraction suitable for database construction. Seven hundred and fifty two aged bloodstains on filter paper were randomly divided into
C Alliot et al.
Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine, 74, 56-60 (2013-01-31)
(82)Rb is a positron-emitting radionuclide widely used in nuclear cardiology. One great advantage is its availability through a generator loaded with (82)Sr. (82)Sr can be produced in a high energy cyclotron by irradiating rubidium chloride target with proton beam. In
Shi-Chao Zhang et al.
eLife, 9 (2020-04-01)
Acute phase reactants (APRs) are secretory proteins exhibiting large expression changes in response to proinflammatory cytokines. Here we show that the expression pattern of a major human APR, that is C-reactive protein (CRP), is casually determined by DNMT3A and TET2-tuned
Al-Anoud Jaber et al.
Annals of clinical microbiology and antimicrobials, 8, 2-2 (2009-01-17)
Ethambutol (EMB) is a first-line drug for the treatment of tuberculosis (TB). Resistance to EMB in Mycobacterium tuberculosis isolates is mediated by mutations in several genes involved in arabinan synthesis notably three emb (arabinosyl transferase) and iniA (isoniazid-inducible) genes. Most
Todd W Osmundson et al.
Molecular ecology resources, 13(1), 66-74 (2012-11-06)
The ubiquity, high diversity and often-cryptic manifestations of fungi and oomycetes frequently necessitate molecular tools for detecting and identifying them in the environment. In applications including DNA barcoding, pathogen detection from plant samples, and genotyping for population genetics and epidemiology
L Verheyen et al.
Aquatic toxicology (Amsterdam, Netherlands), 124-125, 66-71 (2012-08-21)
The Free Ion Activity Model (FIAM) predicts that cadmium (Cd) uptake by organisms is identical for solutions with the same free Cd(2+) concentration and inorganic composition. Clear exceptions to the FIAM have been shown for Cd uptake by plant roots
Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechniques 10(4): 506-13 (April 1991).
P Sean Walsh et al.
BioTechniques, 54(3), 134-139 (2013-04-20)
M S Kariapper et al.
Biochemical and biophysical research communications, 284(3), 563-567 (2001-06-09)
Lipoxygenases contain a unique nonheme iron cofactor with a redox role in the catalyzed reaction. The conditions for the extraction of the metal atom were investigated for one of the soybean lipoxygenase isoenzymes. Removal of the iron by o-phenanthroline was
Kenneth M Y Leung et al.
Marine pollution bulletin, 57(6-12), 790-800 (2008-03-11)
The artificial mussel (AM), a novel chemical sampling device, has been developed for monitoring dissolved trace metals in marine environments. The AM consists of Chelex-100 suspended in artificial seawater within Perspex tubing and enclosed with semi-permeable polyacrylamide gel at both
Bo Wang et al.
Journal of phycology, 56(3), 618-629 (2020-01-23)
Iron (Fe) is essential for phytoplankton growth and photosynthesis, and is proposed to be an important factor regulating algal blooms under replete major nutrients in coastal environments. Here, Skeletonema costatum, a typical red-tide diatom species, and Chlorella vulgaris, a widely
Sheng-Jie Nie et al.
Yi chuan = Hereditas, 29(11), 1373-1377 (2007-11-09)
To investigate the feasibility of DNA analysis from free margin of the nail, genomic DNA was extracted from the free margin of nail clipping of 10 volunteers using the proteinase K/SDS -based organic method, the Chelex-100 method, or a combined
Impact of 970 nm photobiomodulation therapy on wound healing in cellular models of hidradenitis suppurativa.
Luisa Zupin et al.
Lasers in medical science, 36(3), 691-698 (2020-07-11)
Norio Onikura et al.
Environmental toxicology and chemistry, 27(2), 266-271 (2008-03-20)
Toxicity of an industrial effluent was characterized using a toxicity identification method established at our research institute. Toxicity was evaluated using the Ceriodaphnia dubia survival test. The industrial effluent chosen in the present study had toxic effects on C. dubia
Johannes Fels et al.
International journal of molecular sciences, 20(10) (2019-05-17)
Cadmium (Cd2+) in the environment is a significant health hazard. Chronic low Cd2+ exposure mainly results from food and tobacco smoking and causes kidney damage, predominantly in the proximal tubule. Blood Cd2+ binds to thiol-containing high (e.g., albumin, transferrin) and
Vit Molva et al.
Insect science, 27(2), 266-275 (2018-08-14)
The interaction of house dust mites (HDM) and microorganisms is the key factor in the survival of these mites in human-made environments. Spent growth medium (SPGM) provides the rest of the diet, along with dead mite bodies and microorganisms. SPGM
Rizwan Mahmud et al.
Journal of molecular microbiology and biotechnology, 29(1-6), 43-56 (2019-12-19)
The human gut is inhabited by several hundred different bacterial species. These bacteria are closely associated with our health and well-being. The composition of these diverse commensals is influenced by our dietary intakes. Non-caloric artificial sweeteners (NAS) have gained global
Zhaoyuan Chen et al.
The Journal of biological chemistry, 292(13), 5519-5531 (2017-02-23)
Manganese contributes to anti-oxidative stress particularly in catalase-devoid bacteria, and DtxR family metalloregulators, through sensing cellular Mn
T P Ryan et al.
Journal of biochemical toxicology, 8(1), 33-39 (1993-03-01)
The effects of transition metals on nonenzymatic and ceruloplasmin catalyzed epinephrine oxidation were investigated by studying rates of epinephrine oxidation in purified buffers and in the presence of metal chelating agents. We found that epinephrine does not "autoxidize" in sodium
Geraldine S C Turner et al.
Analytica chimica acta, 739, 37-46 (2012-07-24)
Three adsorbents (Chelex-100, manganese dioxide [MnO(2)] and Metsorb), used as binding layers with the diffusive gradient in thin film (DGT) technique, were evaluated for the measurement of inorganic uranium species in synthetic and natural waters. Uranium (U) was found to
Gems in 2013.
Nathan Blow
BioTechniques, 54(3), 111-111 (2013-04-20)
Ram H Nagaraj et al.
Biochimica et biophysica acta, 1804(4), 829-838 (2009-12-23)
Human lens proteins (HLP) become chemically modified by kynurenines and advanced glycation end products (AGEs) during aging and cataractogenesis. We investigated the effects of kynurenines on AGE synthesis in HLP. We found that incubation with 5 mM ribose or 5
S Meshinchi et al.
Journal of steroid biochemistry, 34(1-6), 315-317 (1989-01-01)
Cytosol preparations contain an endogenous heat-stable factor which stabilizes the glucocorticoid receptor in its untransformed, non DNA-binding form. Elution of a partially purified preparation of this stabilizing factor through a metal chelating resin (Chelex-100) leads to the loss of its
Florin Stanciu et al.
Croatian medical journal, 50(3), 321-325 (2009-05-30)
To determine allele frequencies' distribution for 15 short tandem repeat (STR) loci in a population sample of 1910 unrelated individuals from the region of Wallachia, South Romania. DNA was isolated using Chelex 100 method and an adapted version of AGOWA
Yan-xia Guo et al.
Fa yi xue za zhi, 25(1), 42-43 (2009-04-29)
To improve DNA extraction from human epithelium. Chelex-100, Chelex-100 organic combined and Chelex-100 magnetism pearl combined methods were adopted according to the situation of each case. With the stepwise extracting protocol, the trace amount of DNA was analyzed most efficiently
Fethiye Gode et al.
Bioresource technology, 99(6), 1981-1991 (2007-05-08)
In the present study, adsorption of Cr(III) and Cr(VI) on Pumice (Pmc), Yarikkaya (YK) brown coal, Chelex-100, and Lewatit MP 62 is examined at room temperature and at initial chromium concentration of 1.0 x 10(-3) mol/L. Column method was carried
Weijia Li et al.
Analytical chemistry, 81(14), 5889-5895 (2009-06-19)
A cesium-specific diffusive gradients in thin films (DGT) technique was developed using copper ferrocyanide (CFCN) as the binding agent. Two types of DGT binding phases were evaluated, one by immobilizing CFCN on Chelex 100 resin gels (Chelex-CFCN) and the other
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