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A new technique for the assay of infectivity of human adenovirus 5 DNA.
F L Graham et al.
Virology, 52(2), 456-467 (1973-04-01)
Patrick Ovando-Roche et al.
Bio-protocol, 5(13) (2015-07-05)
Ubiquitination is the first step of the ubiquitin-proteasome pathway that regulates cells for their homeostatic functions and is an enzymatic, protein post-translational modification process in which ubiquitin is transferred to a target protein substrate by a set of three ubiquitin
E Calleri et al.
Chembiochem : a European journal of chemical biology, 15(8), 1154-1160 (2014-05-07)
Resveratrol, a modulator of several signaling proteins, can exert off-target effects involving the peroxisome proliferator-activated receptor (PPAR) transcription factors. However, evidence for the direct interaction between this polyphenol and PPARs is lacking. Here, we addressed the hypothesis that resveratrol and
A Calcium Phosphate Transfection
Ausubel, F. et al (ed.)
Short Protocols in Molecular Biology, 3rd, 9-9 (1995)
Hillel Galitzer et al.
BMC biology, 7, 17-17 (2009-04-29)
The parathyroid calcium receptor determines parathyroid hormone secretion and the response of parathyroid hormone gene expression to serum Ca2+ in the parathyroid gland. Serum Ca2+ regulates parathyroid hormone gene expression in vivo post-transcriptionally affecting parathyroid hormone mRNA stability through the
M Serrano et al.
Cell, 88(5), 593-602 (1997-03-07)
Oncogenic ras can transform most immortal rodent cells to a tumorigenic state. However, transformation of primary cells by ras requires either a cooperating oncogene or the inactivation of tumor suppressors such as p53 or p16. Here we show that expression
M Wigler et al.
Cell, 14(3), 725-731 (1978-07-01)
Previous studies from our laboratories have demonstrated the feasibility of transferring the thymidine kinase (tk) gene from restriction endonuclease-generated fragments of herpes simplex virus (HSV) DNA to cultured mammalian cells. In this study, high molecular weight DNA from cells containing
Munjin Kwon et al.
Methods in molecular biology (Clifton, N.J.), 1018, 107-110 (2013-05-18)
The calcium phosphate transfection is a widely used method for introducing foreign DNA plasmids into cells. Mechanisms underlying this transfection method are not yet defined; however, DNA-calcium phosphate precipitates are internalized by the cells and DNA is efficiently expressed in
Vincenzo Lupo et al.
Human molecular genetics, 18(23), 4603-4614 (2009-09-12)
Mutations in SH3TC2 (KIAA1985) cause Charcot-Marie-Tooth disease (CMT) type 4C, a demyelinating inherited neuropathy characterized by early-onset and scoliosis. Here we demonstrate that the SH3TC2 protein is present in several components of the endocytic pathway including early endosomes, late endosomes
Elena Popugaeva et al.
Molecular neurodegeneration, 10, 37-37 (2015-08-16)
Alzheimer disease (AD) is a disease of lost memories. Mushroom postsynaptic spines play a key role in memory storage, and loss of mushroom spines has been proposed to be linked to memory loss in AD. Generation of amyloidogenic peptides and
Use of retroviral-mediated gene transfer to deliver and test function of chimeric antigen receptors in human T-cells
Parente-Pereira A C, et al.
Journal of biological methods, 1(2), e7-e7 (2014)
Denise A Carbonaro et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 22(3), 607-622 (2013-11-22)
Gene transfer into autologous hematopoietic stem cells by γ-retroviral vectors (gRV) is an effective treatment for adenosine deaminase (ADA)-deficient severe combined immunodeficiency (SCID). However, current gRV have significant potential for insertional mutagenesis as reported in clinical trials for other primary
Catarina Pinto et al.
Journal of biotechnology, 300, 70-77 (2019-06-01)
Adeno-associated viral vectors (AAV) for gene therapy applications are gaining momentum, with more therapies moving into later stages of clinical development and towards market approval, namely for cancer therapy. The development of cytotoxic vectors is often hampered by side effects
Ziyou Lin et al.
The EMBO journal, 39(12), e103181-e103181 (2020-05-06)
N6-methyladenosine (m6 A) is an abundant nucleotide modification in mRNA, known to regulate mRNA stability, splicing, and translation, but it is unclear whether it is also has a physiological role in the intratumoral microenvironment and cancer drug resistance. Here, we
Paolo Conrotto et al.
Molecular oncology, 5(6), 527-537 (2011-09-02)
The transcription factor SOX11 is a novel diagnostic marker for mantle cell lymphoma (MCL), distinguishing this aggressive tumor from potential simulators. Recent data also show that the level of SOX11 correlates to in vitro growth properties in MCL, as well
Atar Lev et al.
The Journal of experimental medicine, 218(3) (2020-11-25)
The T cell receptor (TCR) signaling pathway is an ensemble of numerous proteins that are crucial for an adequate immune response. Disruption of any protein involved in this pathway leads to severe immunodeficiency and unfavorable clinical outcomes. Here, we describe
Marine Theret et al.
The EMBO journal, 36(13), 1946-1962 (2017-05-19)
Control of stem cell fate to either enter terminal differentiation versus returning to quiescence (self-renewal) is crucial for tissue repair. Here, we showed that AMP-activated protein kinase (AMPK), the master metabolic regulator of the cell, controls muscle stem cell (MuSC)
Knock-down of SOX11 induces autotaxin-dependent increase in proliferation in vitro and more aggressive tumors in vivo
Conrotto P, et al.
Molecular Oncology, 5(6), 527-537 (2011)
Melanie Galla et al.
Journal of virology, 82(6), 3069-3077 (2008-01-18)
Analyzing cellular restriction mechanisms provides insight into viral replication strategies, identifies targets for antiviral drug design, and is crucial for the development of novel tools for experimental or therapeutic delivery of genetic information. We have previously shown that retroviral vector
A new chimeric protein represses HIV-1 LTR-mediated expression by DNA methylase
Martinez-Colom A, et al.
Antiviral research, 98(3), 394-400 (2013)
Virginie Mournetas et al.
Journal of biological methods, 3(4), e55-e55 (2016-10-04)
Gene silencing techniques, including RNA interference methodologies, are widely used in reverse genetics to study the role of specific genes in biological processes. RNA interference has become easier to implement thanks to the RNAi Consortium (TRC), which has developed libraries
Peter Naniima et al.
PLoS biology, 19(11), e3001423-e3001423 (2021-11-05)
Herpesviruses cause severe diseases particularly in immunocompromised patients. Both genome packaging and release from the capsid require a unique portal channel occupying one of the 12 capsid vertices. Here, we report the 2.6 Å crystal structure of the pentameric pORF19
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