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Product Information Sheet - C6164
Cell freezing Media - Product Information Sheet
Cell freezing Media
sterile-filtered, suitable for cell culture
Product Number C6039
Cell Freezing Medium-Glycerol 1
Product Number
Product Information Sheet - C6039
Cell freezing Media - Product Information Sheet
Cell freezing Media
sterile-filtered, suitable for cell culture
Product Number C6039
Cell Freezing Medium-Glycerol 1
Product Number
Product Information Sheet - C6295
Cell freezing Media - Product Information Sheet
Cell freezing Media
sterile-filtered, suitable for cell culture
Product Number C6039
Cell Freezing Medium-Glycerol 1
Product Number
New, combined lysis and purification reaction simplifies recombinant protein purification with magnetic beads
unwanted cell debris and permit recovery of the
clarified lysate. The final step, purification, is frequently
performed using affinity media specific for expressed
epitope tags. Agarose-based media have
Product Information Sheet - C2999
during the
freezing, storage, and thawing process. Through
modulating the cellular biochemical response to the
cryopreservation process, these media provide
enhanced cell viability and functionality
Product Information Sheet - C2874
during the
freezing, storage, and thawing process. Through
modulating the cellular biochemical response to the
cryopreservation process, these media provide
enhanced cell viability and functionality
Product Information Sheet - C3124
during the
freezing, storage, and thawing process. Through
modulating the cellular biochemical response to the
cryopreservation process, these media provide
enhanced cell viability and functionality
3dGRO¿ Organoid Freeze Medium
homemade organoid freezing media formulations.
Storage
Aliquot and store the 3dGRO™ Organoid Freeze Medium at -20°C.
Thaw at room temperature or 2-8°C overnight. Avoid mutiple freeze
thaw cycles
Cellvento 4CHO Fed-batch Medium
re-suspend cells in cold
freezing medium at 1x107 - 2x107 viable cells/mL,
and transfer the cell suspension into sterile cryovials
with 1mL each vial.
• Freezing procedure with a freezing container
ESGRO Complete Clonal Grade Medium: Serum-Free Medium for Mouse Embryonic Stem Cell Culture
FBS-containing media at a density of 25 cells/cm2 (on
gelatinized TC plastic; note that cells plated into a particular
media had already been passaged 5 times at standard
densities in that media). Media
Product Information Cellvento™ CHO-220 Medium EMD
and resuspend cells in cold
freezing medium at 1×107 – 2×107 viable cells/mL,
and transfer the cell suspension into sterile cryovials,
1 mL per vial.
• Freezing procedure with a freezing container
Product Information Sheet - CHODHFR
Prepare freezing media: Growth Media + 7% DMSO. •
Remove 600 µL sample from stock cell culture and count by •
trypan blue exclusion.
Calculate the volume of cell stock and freezing media •
needed
Article: Freezing Down Time in Bioprocessing
HD Cryo media have to protect the cells
from stress during the freezing process,
which is a significant factor in the viability
drop-off after freeze-thaw. The idea is
that the post-thaw cell population
Product Information Cellvento™ CHO-100 Medium
each
adaptation step are recommended to ensure that cells
appropriately adjust to their new media environments.
Note: The freezing procedure can be standardized using an
automatic cooling instrument
Process Guidance Cellvento™ CHO-210 Medium
ensure that cells
appropriately adjust to their new media environments.
3. Cryopreservation
Viable cell banks may be created by freezing cells in 90 %
Cellvento™ CHO-210 medium and cell culture grade
Product Information Cellvento®4CHO Chemically defined cell culture medium
to ensure that cells appropriately adjust to their new
media environments.
Cryopreservation
Viable cell banks may be created by freezing cells in
90% Cellvento® 4CHO medium and cell culture grade
Process Guidance - Cellvento CHO 210
re-suspend cells in cold freezing medium at 1 x 10⁷ – 2 x 10⁷
viable cells/mL, and transfer the cell suspension into sterile cryovials, 1 mL per vial.
• Freezing procedure with a freezing container
Process Guidance Cellvento CHO-220 Medium EMD
re-suspend cells in cold
freezing medium at 1 x 10⁷–2 x 10⁷ viable cells/mL,
and transfer the cell suspension into sterile cryovials,
1 mL per vial.
• Freezing procedure with a freezing container
Strategies for Upstream Intensification
process.
minimize adaptation when transferring cells from one
step to the next:
• Cryomedium must support freezing and thawing of
cells without cell damage.
• Expansion medium must have a high
Process Guidance Cellvento™ CHO-200 Medium
Adaptation complete when cells maintain
normal doubling time; VCD ≥ 90 % over
at least 2 passages
3. Cryopreservation
Viable cell banks may be created by freezing cells in 90 % Cellvento™ CHO-200
Stem Cellutions Newsletter: 2006, Volume 1
confirmed (not shown).
Freezing and Thawing the Cells
We have optimized standard freezing solutions
for the cryopreservation of the mouse ES cells
to optimize recovery of cells grown in ESGRO
Complete
Product Information Cellvento™ CHO-200 Medium
and resuspend cells in cold
freezing medium at 5×106 – 1×107 viable cells/mL,
and transfer the cell suspension into sterile cryovials,
1 mL per vial.
• Freezing procedure with a freezing container
Cellvento®4CHO-X Product Information
that cells
appropriately adjust to their new media environments.
Cryopreservation
Viable cell banks may be created by freezing cells in
92.5 % Cellvento® 4CHO-X Expansion Medium and cell
culture
Product Information Sheet - 103840
that cells
appropriately adjust to their new media environments.
Cryopreservation
Viable cell banks may be created by freezing cells in
92.5 % Cellvento® 4CHO-X Expansion Medium and cell
culture
Product Information Sheet - CHOK1
10
v) Calculate the volume of cell stock and Freezing Medium needed to obtain 5-10e6 cells per cryovial
(1ml volume).
Note: Once cell preparation is initiated, work must proceed quickly
PluriSTEM Human ES/iPS Medium
mL of PluriSTEM™ Freezing Medium may be used to freeze colonies from 2 wells of a
6-well plate. However, if the colonies are greater than 80% confluent, 1 mL of PluriSTEM™
Freezing Medium may be used
A Novel Serum-free and Xeno-free Differentiation Medium for Accelerated Osteogenic Differentiation of Human Mesenchymal Stem Cells
100,000 beads
Day 1:
Split1: 15 cell culture media
Day 7:
Pool
Day 7:
Split 2: 15 cell culture media
Day 14:
Pool
Day 14:
Split 3: 15 cell culture media
Day 21:
Immunostaining for
Osteocalmcin
Product Information Sheet - 102485
and resuspend cells in cold
freezing medium at 1×107–2×107 viable cells/mL,
and transfer the cell suspension into sterile cryovials,
1 mL per vial.
• Freezing procedure with a freezing container
containing
Data Sheet
Rapidly transfer 1 mL of the cell suspension to a 2 mL cryovial and cap tightly.
6. Place vials immediately in cryovial freezing container prechilled at 4 °C and freeze cells at a rate of –1 °C to
–3
Process Guidance Cellvento®4CHO Fed-batch Medium
CHO-200 media system evaluation
1. Direct media adaptation of CHO-S cells
Some cells may adapt directly into Cellvento® CHO-200 medium. Cells should be seeded at 2.5 x 105–3.5 x 105 cells/mL,
then
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