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Showing 1-30 of 95 results for "cell freezing media" within Papers
Guoliang Meng et al.
Methods in molecular biology (Clifton, N.J.), 1516, 47-56 (2016-04-02)
Efficient cryopreservation of human pluripotent stem cells (hPSCs) in chemically defined, xeno-free conditions is highly desirable for medical research and clinical applications such as cell-based therapies. Here we present a simple and effective slow freezing-rapid thawing protocol for the cryopreservation
Takamichi Miyazaki et al.
Genesis (New York, N.Y. : 2000), 52(1), 49-55 (2013-11-21)
Human pluripotent stem cells (hPSCs) have the potential for unlimited expansion and differentiation into cell types of all three germ layers. Cryopreservation is a key process for successful application of hPSCs. However, the current conventional method leads to poor recovery
Fenlu Zhu et al.
Cytotherapy, 17(12), 1813-1819 (2015-10-12)
Removing DMSO post-thaw results in: reduced infusion reactions, improved recovery and stability of viable CD34+ cells. Validated methods use 5%-8.3% Dextran 40 with 2.5%-4.2% HSA for this purpose. Recent shortages of clinical grade Dextran require identification of suitable alternatives. PBPC
Caroline Rauch et al.
ALTEX, 28(4), 305-316 (2011-12-02)
The search for alternatives to the use of fetal bovine serum (FBS) in cell and tissue culture media has become a major goal in terms of the 3R principles in order to reduce or to avoid harvesting of FBS from
Xiangyun Li et al.
Human reproduction (Oxford, England), 24(3), 580-589 (2008-12-06)
Efficient slow freezing protocols within serum-free and feeder-free culture systems are crucial for the clinical application of human embryonic stem (hES) cells. Frequently, however, hES cells must be cryopreserved as clumps when using conventional slow freezing protocols, leading to lower
Marwen Moussa et al.
Applied and environmental microbiology, 73(20), 6508-6518 (2007-09-04)
The relationship between membrane permeability, changes in ultrastructure, and inactivation in Escherichia coli strain K-12TG1 cells subjected to high hydrostatic pressure treatment at room and subzero temperatures was studied. Propidium iodide staining performed before and after pressure treatment made it
K Sawada et al.
International journal of oral and maxillofacial surgery, 44(8), 1060-1066 (2015-04-15)
The autoclaving, pasteurization, and freezing of bone grafts to remove bacteria and viruses, and for preservation, respectively, is considered to alter biological properties during graft consolidation. Fresh bone grafts release paracrine-like signals that are considered to support tissue regeneration. However
Tomohiro Isobe et al.
Animal science journal = Nihon chikusan Gakkaiho, 86(7), 661-665 (2014-12-10)
The optimization of single-embryo culture conditions is very important, particularly in the in vitro production of bovine embryos using the ovum pick-up (OPU) procedure. The purpose of this study was to examine the development of embryos derived from oocytes obtained
Duncan C Miller et al.
Current protocols in stem cell biology, 55(1), e125-e125 (2020-09-22)
Great progress has been made with protocols for the differentiation and functional application of hPSC-cardiomyocytes (hPSC-CMs) in recent years; however, the cryopreservation and recovery of hPSC-CMs still presents challenges and few reports describe in detail the protocols and general workflow.
Alexander Meissner et al.
Methods in molecular biology (Clifton, N.J.), 482, 3-19 (2008-12-18)
Pluripotent embryonic stem (ES) cell lines were first isolated over 25 years ago and remain an essential tool in molecular and developmental biology to this day. In particular, the use of homologous recombination and subsequent generation of ES-derived mice has
Maristela Delgado Orellana et al.
Cryobiology, 71(1), 151-160 (2015-02-03)
The therapeutic use of human embryonic stem cells (hESCs) is dependent on an efficient cryopreservation protocol for long-term storage. The aim of this study was to determine whether the combination of three cryoprotecting reagents using two freezing systems might improve
Melany Jackson et al.
Methods in molecular biology (Clifton, N.J.), 633, 1-18 (2010-03-06)
Embryonic stem (ES) cells are pluripotent cells isolated from the inner cell mass of the pre-implantation blastocyst. They have the capacity to undergo indefinite rounds of self-renewing cell division and differentiate into all the cell lineages of the developing embryo.
Lia H Campbell et al.
Cryobiology, 64(3), 240-244 (2012-03-01)
Dimethylsulfoxide, the most commonly employed cryoprotectant for cells, has well documented cytotoxic effects in patients. Among the compounds available that may provide protection to cells and tissues during preservation with less cytotoxicity is trehalose. Some animals, such as brine shrimp
Kuldip S Sidhu et al.
Current protocols in stem cell biology, Chapter 1, Unit 1A-Unit 1A (2008-09-05)
The pluripotent nature of human embryonic stem cells (hESC) makes them very attractive as a source of various cell types that could be used therapeutically in regenerative medicine. However, eliminating all sources of contamination, animal-derived or human cell-derived, during hESC
Alice Roffi et al.
BioMed research international, 2014, 692913-692913 (2014-08-20)
PRP cryopreservation remains a controversial point. Our purpose was to investigate the effect of freezing/thawing on PRP molecule release, and its effects on the metabolism of chondrocytes and synoviocytes. PRP was prepared from 10 volunteers, and a half volume underwent
Christian Moya Gamboa et al.
Cells, 10(12) (2021-12-25)
The liver is among the principal organs for glucose homeostasis and metabolism. Studies of liver metabolism are limited by the inability to expand primary hepatocytes in vitro while maintaining their metabolic functions. Human hepatic three-dimensional (3D) organoids have been established
F C Bach et al.
European cells & materials, 32, 163-180 (2016-08-31)
During intervertebral disc (IVD) maturation, notochordal cells (NCs) are replaced by chondrocyte-like cells (CLCs) in the nucleus pulposus, suggesting that NCs play a role in maintaining tissue health. Affirmatively, NC-conditioned medium (NCCM) exerts regenerative effects on CLC proliferation and extracellular
Scott L Friedman et al.
Methods in molecular biology (Clifton, N.J.), 2669, 129-162 (2023-05-29)
Hepatic stellate cells (HSCs) are the major cellular source of extracellular matrix production in the liver. Therefore, this cell population has received considerable attention in studies investigating fundamental features of hepatic fibrosis. However, the limited supply and ever-increasing demand for
Peter V Glovinski et al.
Cytotherapy, 19(2), 222-234 (2016-11-27)
Platelet lysates (PL) represent a promising replacement for xenogenic growth supplement for adipose-derived stem cell (ASC) expansions. However, fresh platelets from human blood donors are not clinically feasible for large-scale cell expansion based on their limited supply. Therefore, we tested
Fei Xiong et al.
Journal of pharmaceutical sciences, 103(12), 4030-4037 (2014-10-23)
Over the last decade, various magnetic nanomaterials have been developed as magnetic resonance imaging (MRI) contrast agents; the greatest challenges encountered for clinical application have been insufficient stability. In this paper, a lyophilization method for 2, 3-dimercaptosuccinic acid-modified iron oxide
H Louise Ashurst et al.
Experimental physiology, 93(2), 223-236 (2007-10-16)
The gastrin/CCK receptor (CCK2R) mediates the physiological functions of gastrin in the stomach, including stimulation of acid secretion and cellular proliferation and migration, but little is known about the factors that regulate its expression. We identified endogenous CCK2R expression in
H B Klinke et al.
Biotechnology and bioengineering, 81(6), 738-747 (2003-01-17)
Alkaline wet oxidation (WO) (using water, 6.5 g/L sodium carbonate and 12 bar oxygen at 195 degrees C) was used as pretreatment method for wheat straw (60 g/L), resulting in a hydrolysate and a cellulosic solid fraction. The hydrolysate consisted
Susan Noell et al.
The European journal of neuroscience, 26(8), 2109-2118 (2007-10-12)
Agrin is a heparan sulfate proteoglycan of the extracellular matrix and is known for organizing the postsynaptic differentiation of the neuromuscular junction. Increasing evidence also suggests roles for agrin in the developing CNS, including the formation and maintenance of the
Bumjin Namkoong et al.
Acta biomaterialia, 31, 301-311 (2015-12-18)
The experimental systems that recapitulate the complexity of native tissues and enable precise control over the microenvironment are becoming essential for the pre-clinical tests of therapeutics and tissue engineering. Here, we described a strategy to develop an in vitro platform
A R Burns et al.
Journal of cell science, 113 ( Pt 1), 45-57 (1999-12-11)
Intercellular junctions have long been considered the main sites through which adherent neutrophils (PMNs) penetrate the endothelium. Tight junctions (TJs; zonula occludens) are the most apical component of the intercellular cleft and they form circumferential belt-like regions of intimate contact
Karthiyaini Damodharan et al.
Canadian journal of microbiology, 61(11), 837-850 (2015-09-19)
In this study, we evaluated the probiotic properties of Lactobacillus plantarum, Lactobacillus pentosus, and Lactobacillus fermentum strains isolated from fermented radish. All the strains survived the simulated oro-gastrointestinal transit condition and showed significantly higher adherence to Caco-2 cells compared with
Guenther Schuldt Filho et al.
Journal of periodontology, 86(6), 827-834 (2015-03-20)
Demineralized bone matrix (DBM) is used for the treatment of osseous defects. Conditioned medium from native bone chips can activate transforming growth factor (TGF)-β signaling in mesenchymal cells. The aim of this study is to determine whether processing of native
Simple freezing procedure for storage in serum-free media of cultured and tumor cells of mouse.
Waymouth, C. and Varnum, D.
TCA Manual, 2(1), 311-313 (1976)
The Effects of Freezing Media on the Characteristics of Male and Female Chicken Primordial Germ Cell Lines.
Ecker, et al.
Life (Basel, Switzerland), 13 (2023)
A V Nikolenko et al.
Cryo letters, 34(5), 527-534 (2014-01-23)
Oxyethyl derivatives of polyol class members such as glycerol and ethylene glycol with various polymerization degrees possess low toxicity and cryoprotective properties via an exocellular mechanism. To develop the cryoprotective media based on non-penetrating cryoprotectants, we studied cryoprotective and physico-chemical
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