Showing 1-30 of 4086 results for "centrifugation"
M Delahaye et al.
Biotechnology and bioengineering, 112(5), 997-1011 (2014-12-30)
An ultra scale-down method is described to determine the response of cells to recovery by dead-end (batch) centrifugation under commercially defined manufacturing conditions. The key variables studied are the cell suspension hold time prior to centrifugation, the relative centrifugal force...
Akiyuki Hasegawa et al.
Journal of biomedical materials research. Part A, 103(12), 3825-3833 (2015-06-23)
Three-dimensional (3D) tissues can be reconstructed by cell sheet technology, and various clinical researches using these constructed tissues have already been initiated to regenerate damaged tissues. While 3D tissues can be easily fabricated by layering cell sheets, the attachment period...
Ariane E Erickson et al.
Carbohydrate polymers, 134, 467-474 (2015-10-03)
The inability to produce large quantities of nanofibers has been a primary obstacle in advancement and commercialization of electrospinning technologies, especially when aligned nanofibers are desired. Here, we present a high-throughput centrifugal electrospinning (HTP-CES) system capable of producing a large...
Elodie Bouju et al.
Journal of chromatography. A, 1409, 70-78 (2015-07-27)
Centrifugal Partition Chromatography (CPC) is a purification technique using a biphasic liquid system. As a preparative separation technique, scale-up is of primary concern. Once the separation is optimized on a lab-scale instrument, the scale-up transfer is quite straightforward simply using...
Qiliang Zhou et al.
Tissue engineering. Part C, Methods, 24(9), 524-533 (2018-08-14)
The outcomes of tracheal transplantation for the treatment of airway stenosis are unsatisfactory. We investigated the feasibility of regeneration of the trachea using a rat decellularized tracheal scaffold and mouse-induced pluripotent stem (iPS) cells for in vivo transplantation. The rat...
Rosemary Marques et al.
Journal of labelled compounds & radiopharmaceuticals, 58(6), 261-263 (2015-05-13)
Tritium radiopharmaceuticals are often used in drug development because of their desirable specific activity. The inherent instability of these radioactive tracers often leads to a requirement to purify prior to use. Purification methodologies such as preparative chromatography and solid/liquid extractions...
Ewa Stępień et al.
Biochemia medica, 25(2), 222-229 (2015-06-26)
Centrifugation is an essential step for plasma preparation to remove residual elements in plasma, especially platelets and platelet-derived microparticles (PMPs). Our working hypothesis was that centrifugation as a preanalytical step may influence some coagulation parameters. Healthy young men were recruited...
Raena Morley et al.
Journal of chromatography. A, 1501, 26-38 (2017-05-05)
Trapping multiple dual mode centrifugal partition chromatography (trapping MDM CPC) is an alternative to isocratic pulse injections for the separation of intermediately-eluting components from complex mixtures using liquid-liquid chromatography. In this work, a throughput maximization strategy is developed and validated...
Ransi Ann Abraham et al.
Biochemia medica, 29(2), 020708-020708 (2019-06-22)
Preanalytical conditions are critical for blood sample integrity and poses challenge in surveys involving biochemical measurements. A cross sectional study was conducted to assess the stability of select biomarkers at conditions that mimic field situations in surveys. Blood from 420...
Ulrika Qundos et al.
Journal of proteomics, 95, 46-54 (2013-05-02)
Several biobanking initiatives have emerged to create extensive collections of specimen for biomedical studies and various analytical platforms. An affinity proteomic analysis with antibody suspension bead arrays was conducted to investigate the influence of the pre-analytical time and temperature conditions...
Johannes Goll et al.
Journal of chromatography. A, 1496, 68-79 (2017-04-02)
The preparative separation of intermediately-eluting components in liquid-liquid chromatography is commonly performed with isocratic batch injections, a technique which often leads to low yield and/or purity as a result of peak overlap. Two-column trapping multiple dual mode centrifugal partition chromatography...
Supriya Lath et al.
Chemosphere, 222, 671-678 (2019-02-09)
Measurement and reporting of concentrations of contaminants of emerging concern such as per- and polyfluoroalkyl substances (PFASs), including perfluorooctanoic acid (PFOA), is an integral part of most investigations. Occurrence of sorption losses of PFAS analytes onto particular laboratory-ware (e.g. glass...
Sung-Woo Lee et al.
Journal of chromatography. A, 1373, 25-30 (2014-12-02)
Circulating tumor cells (CTCs) are defined as cells that have detached from a primary tumor and are circulating in the bloodstream. Their isolation and quantification is of great value for cancer prognoses and drug testing. Here, the development of a...
N Šterbenc et al.
Cryobiology, 86, 77-83 (2018-12-07)
The cell membrane of ram spermatozoa is more sensitive to the freezing process than in other species due to its composition. As a result, the quality and viability of frozen thawed ram spermatozoa are often poor, which together with the...
J Krause et al.
Journal of chromatography. A, 1504, 64-70 (2017-05-17)
Recently the Centrifugal Partition Chromatography (CPC) device is investigated as a reactor for biocatalytic reactions, as it enables biocatalyst immobilization without solid support and continuous operation of biphasic reaction systems. However, a detailed determination of the enzymes behavior in the...
Yating Liu et al.
Journal of chromatography. A, 1400, 40-46 (2015-05-16)
As the rapid development of nanotechnology, the magnetic nanospheres modified with special chiral selective ligands show a great potentiality in enantiomeric separation. In this study, magnetic nanospheres modified with task-specific chiral ionic liquid were designed for the separation of chiral...
Je-Seung Jeon et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1011, 108-113 (2016-01-17)
A preparative separation method using consecutive sample injection centrifugal partition chromatography (CPC) was developed to obtain sesamin and sesamolin from defatted sesame meal extracts. A two-phase solvent system consisting of n-hexane-ethyl acetate-methanol-water (8:2:8:2, v/v) was applied in reversed-phase mode (descending...
Lucian C Staicu et al.
Chemosphere, 125, 130-138 (2015-01-07)
Biogenic selenium, Se(0), has colloidal properties and thus poses solid-liquid separation problems, such as poor settling and membrane fouling. The separation of Se(0) from the bulk liquid was assessed by centrifugation, filtration, and coagulation-flocculation. Se(0) particles produced by an anaerobic...
Adriana Urbina et al.
Journal of chromatography. A, 1590, 104-112 (2019-01-12)
Split-flow fractionation (SPLITT) is a family of techniques that separates in the absence of labeling using very low flow rates and force fields, and is therefore expected to minimize cell damage. Although it has been documented that separation methods cause...
David Schibilsky et al.
Artificial organs, 39(8), 723-728 (2015-08-04)
The hemocompatible properties of rotary blood pumps commonly used in mechanical circulatory support (MCS) are widely unknown regarding specific biocompatibility profiles of different pump technologies. Therefore, we analyzed the hemocompatibility indicating markers of an axial flow and a magnetically levitated...
J K Sheehan et al.
The Biochemical journal, 199(3), 581-589 (1981-12-01)
1. Two proteodermatan sulphate species from bovine sclera (fractions PG-I and PG-II) separable by gel chromatography were studied by isopycnic centrifugations in CsCl and Cs(2)SO(4) both in an analytical and a preparative mode. 2. In CsCl, fraction PG-I formed a...
Mehdi Najar et al.
Cytotherapy, 16(9), 1220-1228 (2014-07-30)
Adult human subcutaneous adipose tissue harbors a multipotent stem cell population, the so-called human adipose tissue-derived mesenchymal stromal cells (AT-MSCs). These cells are able to differentiate in vitro into various cell types and possess immunomodulatory features. Yet procedures to obtain...
K Andersson et al.
Plasmid, 13(1), 78-80 (1985-01-01)
Plasmids extracted from bacterial cells by alkaline extraction can easily be isolated from linear DNA by isopycnic centrifugation in CsTFA. This is a fast and simple method which circumvents the use of the intercalating dye, ethidium bromide, and consequently the...
Romana Koszagova et al.
Microbial cell factories, 17(1), 139-139 (2018-09-05)
Physiological aggregation of a recombinant enzyme into enzymatically active inclusion bodies could be an excellent strategy to obtain immobilized enzymes for industrial biotransformation processes. However, it is not convenient to recycle "gelatinous masses" of protein inclusion bodies from one reaction...
L Anel-López et al.
Animal reproduction science, 158, 68-74 (2015-05-24)
Single Layer Centrifugation is a useful technique to select sperm with good quality. The use of selection methods such as Androcoll could become an important tool to improve the quality of sperm samples and therefore to improve other artificial reproductive...
Jameel A Feshitan et al.
Journal of colloid and interface science, 329(2), 316-324 (2008-10-28)
Microbubbles used as contrast agents for ultrasound imaging, vectors for targeted drug delivery and vehicles for metabolic gas transport require better size control for improved performance. Mechanical agitation is the only method currently available to produce microbubbles in sufficient yields...
Dina Tleugabulova et al.
Langmuir : the ACS journal of surfaces and colloids, 22(4), 1852-1857 (2006-02-08)
Recent experimental studies have shown that time-resolved fluorescence anisotropy (TRFA) is a promising methodology for in situ characterization of the surface modification of aqueous silica nanocolloids. Here we provide a more fundamental insight into the principle of this approach and...
Yoshinori Onuki et al.
Chemical & pharmaceutical bulletin, 63(5), 377-383 (2015-05-08)
A mixed preparation consisting of a water-in-oil emulsion-type moisturizing cream and a steroid ointment is frequently prescribed for the treatment of atopic dermatitis. We have investigated the compatibility of moisturizing creams and ointments because there are concerns regarding the physical...
Aneta Wrzyszcz et al.
Platelets, 28(1), 43-53 (2016-09-03)
To date, there has been no ideal method for blood platelet isolation which allows one to obtain a preparation devoid of contaminations, reflecting the activation status and morphological features of circulating platelets. To address these requirements, we have developed a...
Rate zonal centrifugation in a Ficoll gradient.
T G Pretlow et al.
Analytical biochemistry, 29(2), 230-237 (1969-05-01)

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