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Reverse Transcription Protocol (One-step Probe Detection)
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
dNTP Mediated Hot Start PCR Protocol
Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecting group is removed during a heat activation step.
Reverse Transcription Protocol (One-step SYBR Green I Dye Detection)
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
qPCR Gene Expression Using Probe Detection
The most common application for qPCR is the measurement of a gene transcript or copy number quantity relative to one or more reference genes using probe detection.
Standard Reverse Transcription Protocol (Two-step)
Reverse transcription is the analysis of gene expression to measure concentration mrna a gene. there are several challenges such analyses, as differences in halflife between different transcripts, temporal patterns and lack correlation protein.
Primer Concentration Optimization Protocol
Primer Concentration Optimization Protocol is an approach to create a matrix of reactions. This is used to test a range of concentrations for each primer against different concentrations of the partner primer.
Multiplex qPCR Protocol
A protocol that can be used as a basic template for qPCR incorporating up to four detection probes. In these reactions, primers and probe are included at a final concentration of 200 nM and are run using LuminoCt ReadyMix.
qPCR Using a Single Detection Probe Protocol
A protocol that can be used as a basic template for qPCR incorporating a detection probe that is specific to a single target. In these reactions, primers and probe are included at a final concentration of 200 nM and are
Amplification of Damaged DNA with Restorase DNA Polymerase (R1028)
Method for amplification of DNA from damaged DNA sources. Particularly useful for DNA extracted from old samples.
Amplification of Genomic DNA using REDTaq® DNA Polymerase
Method for PCR using genomic DNA or other complex templates
Method for PCR amplification of Bacterial DNA with low contamination using MTP™ Taq DNA Polymerase (D7442)
Method for PCR amplification of Bacterial DNA with low contamination using MTP™ Taq DNA Polymerase (D7442)
Primer Optimization Protocol Using Temperature Gradient
Gradient PCR for assay optimization is to determine the optimum annealing temperature (Ta) of the primers by testing identical reactions containing a fixed primer concentration, across a range of annealing temperatures.
Standard PCR Protocol
Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.
End-Point PCR: Antibody-Mediated Hot Start PCR Protocol with Enhanced Specificity and Yield
Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
qPCR Efficiency Determination Protocol
Optimization of qPCR conditions is important for the development of a robust assay. The two main approaches are optimization of primer concentration and/or annealing temperatures.
qPCR Gene Expression/Copy Number Analysis Protocol Using SYBR Green I Dye Detection
Measuring a target quantity relative to one or more stable reference genes using SYBR Green I dye detection is a common application of qPCR. Below is a standard protocol that can be adapted to specific experimental needs.
The 3'/5' Assay for Analysis of RNA Integrity Protocol
The 3’/5’ integrity assay is a potential first step in the identification of RNA degradation. The assay is particularly useful when a large number of samples are to be analyzed or when the degradation is less than that detected by
SPUD Assay for Detection of Assay Inhibitors Protocol
he SPUD assay is one option for identification of inhibitors that may be present in RNA or DNA samples. The assay is particularly useful when a large number of samples are to be analyzed or when targets are present at
Universal SYBR Green qPCR Protocol
Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions