Library preparation is the first step of next generation sequencing. Before DNA or RNA samples can be sequenced, nucleic acids must be isolated, fragmented, end-repaired, and covalently linked to adapters using ligation or tagmentation methods.
Transplex Whole Transcriptome Amplification FAQs on topics including whole transcriptome steps, RNA source, including archival fixed tissue, library purification, quantitation of the product and downstream applications
Amplification products generated by the TransPlex® WTA and Complete WTA2 kits are suitable for microarray target for expression analyses, and can be readily integrated
into existing NimbleGen workflows.
Transplex Whole Transcriptome Amplification (WTA2) exponentially amplifies RNA producing a double-stranded cDNA library while precisely maintaining differential levels of individual transcripts in test and reference samples.
Whole Genome Amplification can be performed on DNA extracted in many ways. We offer many products for DNA extraction, including the GenElute™ Blood Genomic DNA Kit, GenElute Mammalian Genomic DNA Miniprep Kit and the GenElute Plant Genomic DNA M iniprep.
GenomePlex® Whole Genome Amplification is the method of extracting DNA from the animal sample. GenomePlex® products have been used to amplify genomic DNA from chicken, porcine, bovine, fish, and shrimp source.
Whole blood is a common source of material used to perform genetic analysis. Many times genomic DNA isolated from whole blood samples is of low yield. This protocol is a simple method to isolate DNA from fresh or aged
Genomic DNA from soil samples can be easily damaged by nucleases and contaminating debris resulting in low DNA yields. As a result, the researcher’s ability to perform downstream analysis may be compromised. After isolating DNA from the soil sample, the
Blood cards provide the convenience of archiving small volumes of blood. However, many times genomic DNA from these samples is limited, This protocol provides a simple and convenient method to extract genomic DNA from a blood card. Once the DNA
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