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concentrating zone tlc plates
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Thin-Layer Chromatography
individual plate number
• Easy documentation and archiving of every plate
• Same reliable performance as TLC and HPTLC plates
manufactured
TLC and HPTLC Plates with Concentrating Zone
Thin-Layer
EMD TLC Catalog - 10001523
1980 1981 1984
TLC Plates with Sample
Concentration Zone
The concentration zone layer consists of a 2.5
cm band of wide-pore silica gel particles along
one edge of the plate. The large pore size
105651 - ProteoChrom HPTLC Cellulose sheets, 10 x 10 cm
Ammonia 25% Merck Art. 1.05432
5.3 For staining:
Typical TLC equipment for spraying and dipping (e.g. Merck TLC
sprayer Art. 1.08540, TLC plate heater).
- with ninhydrin:
• Ninhydrin Merck Art. 1.06762
DriSolv®Solvents and TLC Plates
uncompromising quality
in performance, plate after plate, lot after lot. This consistency is
what has made our TLC Plates the standard to which other TLC
Plates are measured. Select from a large variety
Chrombook 2015 - The world of chromatography in your hands
, e.g. oils, cosmetics. Analytical
TLC and HPTLC concentration zone plates provide concentrating areas of 2.5 cm while the concentrating zone of
preparative plates (PLC) is 4 cm in width.
The special
Data Sheet - 43264
samples diffuse into the nutrient media and cause growth-free
inhibition zones to develop on the otherwise thickly covered plates. Repeated tests with pH 6,0, pH 8,0
and pH 7,2 are necessary, as penicillin
Data Sheet - 17186
Interpretation
No zone Clear zone
(17 - 20 mm)
No zone No significant amounts of inhibiting
substances.
Clear zone
(zone
(17 - 20 mm)
No zone significant amounts
HPLC Reagents
20 x 20 Glass TLC 250 25-PK
5608-7 Silica Gel 60 F254 (Prescored to 5x20) 20 x 20 Glass TLC 250 20-PK
11798-7 Silica Gel 60 F254 with 2.5 x 20 cm Concentration Zone 20 x 20 Glass
TLC Explorer Documentation System
aprox. 85 × 85 µm on TLC plate.
Measuring technology Documentation system for TLC plates by using video densitometric measurement
TLC/HPTLC plates sizes Usage of TLC plates with a size of 20 cm
Chromatography Instrumental Techniques
solution containing the sample is applied to a plate. A small amount of an
appropriate solvent (eluent) is poured into a separation chamber. The TLC plate is then placed in
the chamber so that the
User Guide - 152610
together
with the TLC plates as you arranged them.
Recessed grips on all 4 sides of the plate area allow the user to lift the TLC plates from the Base-
plate without touching the TLC plate’s coated surface
User Guide 73070BC - Assurance GDS RGQ - EN
block
PickPen® transfers of concentrated samples to prepared resuspension plate
Transfer of resuspended sample to amplification tubes
Work Zone 3 – Amplification & Detection
User Guide 73065BC 73021BC - Assurance® GDS Rotor - EN
block
PickPen® transfers of concentrated samples to prepared resuspension plate
Transfer of resuspended sample to amplification tubes
Work Zone 3 – Amplification & Detection
Data Sheet 102894 Deoxycholate Lactose Agar
culture medium by the spread-plate or pour-plate method. Incubation: 24
hours at 35 °C aerobically.
Appearance of Colonies Microorganisms
Red, surrounded by zone of precipitate Lactose-positive
Data Sheet - 146700
Antibiotic Generation of Cephalosporin
Concentration
antibiotic [µg]
Inhibition
zone Cephase
plate (mm)*
Inhibition
zone
reference
plate
without
Cephase
(mm)*
Ampicillin N/A 25
Data Sheet - 146076
Antibiotic Generation of Cephalosporin
Concentration
Antibiotic per Disc
Inhibition
zone
Cephase
Plate (mm)*
Inhibition zone
Reference Plate
without Cephase
(mm)*
Ampicillin N/A
Data Sheet - 146539
Antibiotic Generation of Cephalosporin
Concentration
antibiotic [µg]
Inhibition zone
Cephase plate
(mm)*
Inhibition zone
reference plate
without Cephase
(mm)*
Ampicillin N/A 25
Data Sheet -100207
without a
pink-red colored zone in the medium.
Lactose- and/or sucrose-positive microorganisms produce yellow-green colonies mostly surrounded by
a yellow-green colored zone in the medium.
Colonies of
Data sheet-146189
surrounded by a clear zone which may be partially opaque. After
incubation for at least 24 h, an opalescent ring immediately in contact with the colonies may
appear in this clear zone.
Atypical
BPLS Agar (Brilliant-green Phenol-red Lactose Sucrose, 107237
Colonies Microorganisms
Pink surrounded by a red zone Lactose- and sucrose-negative:
Salmonella and others
Yellow-green surrounded by a yellow-green zone Lactose- or sucrose-positive:
E. coli, Citrobacter
Data Sheet 1.05272.0500, 1.05271.0500, 1.05269.0500
poured into plates. Defined quantities
of the antibiotic under examination and an antibiotic standard are applied as spots (cylinder, punched-
hole, paper-discs). On incubation inhibition zones develop
Data Sheet - 6.40016E
1.3 and 2.4 Zones.
Sequential cleaning studies ‐ Sequential cleaning
studies were conducted in the laboratory to further
validate the Zones of Cleanliness. Sterile stainless steel
plates were contaminated
MUELLER-HINTON Agar CLSI
sulfamethoxa-
zole and Enterococcus faecalis ATCC 29212.
Calcium and/or magnesium concentrations are controlled by
obtaining the correct zone diameters with aminoglycoside anti-
biotics and Pseudomonas aeruginosa
Data Sheet - 1.46700.0020 / 1.46700.0120
Antibiotic Generation of Cephalosporin
Concentration
antibiotic [µg]
Inhibition
zone Cephase
plate (mm)*
Inhibition
zone
reference
plate
without
Cephase
(mm)*
Ampicillin N/A 25
Data Sheet - 1.46076.0020 / 1.46076.0120
Antibiotic Generation of Cephalosporin
Concentration
Antibiotic per Disc
Inhibition
zone
Cephase
Plate (mm)*
Inhibition zone
Reference Plate
without Cephase
(mm)*
Ampicillin N/A
Data Sheet - 1.46539.0020 / 1.46539.0200
Antibiotic Generation of Cephalosporin
Concentration
antibiotic [µg]
Inhibition zone
Cephase plate
(mm)*
Inhibition zone
reference plate
without Cephase
(mm)*
Ampicillin N/A 25
Duopath Verotoxins, 104144
Sorbitol-MacConkey
agar plates containing no tellurite or cefixime. Incubate for 18 -
24 h at 35 °C. Using a swab, sweep a few times across the con-
fluent growth area of the plate avoiding mucoid colonies
Data Sheet 146189 ReadyPlate™ CT Baird Parker Agar ISO 6888
surrounded by a clear zone which may be partially opaque. After incubation for at least 24 h, an
opalescent ring immediately in contact with the colonies may appear in this clear zone.
Atypical colonies
Solutions for Mass Spectrometry
component
of this sample is saccharose.
Figure 25
TLC-MS Interface (CAMAG, Switzerland)
for the direct extraction of TLC / HPTLC plates.
Figure 26
Compact mass spectrometer
expression CMS
Data sheet-146137
surrounded by a clear zone which may be partially opaque. After
incubation for at least 24 h, an opalescent ring immediately in contact with the colonies may
appear in this clear zone.
Atypical
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