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R A Burke et al.
Advances in peritoneal dialysis. Conference on Peritoneal Dialysis, 14, 120-123 (2000-01-29)
The presence of mixed disaccharides (maltose and isomaltose) in plasma from uremic patients has been previously investigated using gel-permeation chromatography. However, this method is unable to separate maltose (linked alpha-1-4) from isomaltose (linked alpha-1-6). We describe an alternative method using
Daniel J O'Sullivan et al.
BMC microbiology, 15, 266-266 (2015-11-19)
The aim of this study was to employ high-throughput DNA sequencing to assess the incidence of bacteria with biogenic amine (BA; histamine and tyramine) producing potential from among 10 different cheeses varieties. To facilitate this, a diagnostic approach using degenerate
Verónica Gómez-Pérez et al.
European journal of medicinal chemistry, 89, 362-369 (2014-12-03)
Nine novel symmetrical bispyridinium cyclophanes have been synthesized. They are rigid derivatives with an upper spacer which joins the two exocyclic amino groups, and a lower spacer joining the two positively charged nitrogen atoms. At least one of the two
Paula Florian et al.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 90(3), 449-455 (2012-03-03)
Different cell types have been reported to internalize lactoferrin (Lf) by specific or nonspecific receptors. Our studies focused on the endocytic pathway of human Lf in macrophage-like THP-1 cells. Lactoferrin was found to be internalized by THP-1 cells differentiated with
S Goldberg et al.
Journal of dental research, 73(6), 1168-1172 (1994-06-01)
Whereas previous studies have shown correlations between volatile sulphur compounds (VSC) and bad breath levels, it is probable that other compounds found in the oral cavity may contribute to oral malodor. In the present investigation, the possibility that diamines (cadaverine
Somnuk Bunsupa et al.
The Plant cell, 24(3), 1202-1216 (2012-03-15)
Lysine decarboxylase (LDC) catalyzes the first-step in the biosynthetic pathway of quinolizidine alkaloids (QAs), which form a distinct, large family of plant alkaloids. A cDNA of lysine/ornithine decarboxylase (L/ODC) was isolated by differential transcript screening in QA-producing and nonproducing cultivars
M Cooke et al.
Archives of oral biology, 48(4), 323-327 (2003-03-29)
The concentrations of putrescine, cadaverine, indole and skatole were determined in the saliva of healthy human volunteers upon waking and at time points during the day. Putrescine was found to be the most abundant of the amines studied, followed by
Reeder M Robinson et al.
Archives of biochemistry and biophysics, 550-551, 58-66 (2014-04-29)
The mechanism of Mycobacterium smegmatis G (MbsG), a flavin-dependent l-lysine monooxygenase, was investigated under steady-state and rapid reaction conditions using primary and solvent kinetic isotope effects, substrate analogs, pH and solvent viscosity effects as mechanistic probes. The results suggest that
S Umair et al.
Experimental parasitology, 131(1), 101-106 (2012-03-31)
Catabolism of lysine through the pipecolate, saccharopine and cadaverine pathways has been investigated in L3 and adult Haemonchus contortus and Teladorsagia circumcincta. Both enzymes of the saccharopine pathway (lysine ketoglutarate reductase (LKR) and saccharopine dehydrogenase (SDH)) were active in L3
Andrew J Washkowitz et al.
Development (Cambridge, England), 142(1), 31-40 (2014-12-18)
The maintenance and control of pluripotency is of great interest in stem cell biology. The dual specificity T-box/basic-helix-loop-helix-zipper transcription factor Mga is expressed in the pluripotent cells of the inner cell mass (ICM) and epiblast of the peri-implantation mouse embryo
G A van den Berg et al.
Clinical chemistry, 32(10), 1930-1937 (1986-10-01)
A capillary gas-chromatographic method with nitrogen-phosphorus detection is used to determine simultaneously urinary 1,3-diaminopropane, monoacetyl-1,3-diaminopropane, putrescine, monoacetylputrescine, cadaverine, monoacetylcadaverine, spermidine, N1-acetylspermidine, N beta-acetylspermidine, spermine, N1-acetylspermine, isoputreanine, N-(3-aminopropyl)pyrrolidin-2-one, and putreanine. The compounds are isolated by adsorption onto silica and converted into
K Fujita et al.
Clinical chemistry, 26(11), 1577-1582 (1980-10-01)
This method for assay of polyamines (putrescine, spermidine, and spermine) in large numbers of urine samples is based on preliminary analyte isolation by use of a Dowex 50W column, separation by high-voltage paper electrophoresis, reaction with ninhydrin to detect the
Jack D Sobel et al.
European journal of obstetrics, gynecology, and reproductive biology, 163(1), 81-84 (2012-04-24)
To compare diagnosis of bacterial vaginosis according to the Amsel criteria with measurement by ion mobility spectrometry (IMS) of the biogenic amines that are present in vaginal discharge fluid. Duplicate samples of vaginal fluid were collected from 115 unselected and
D O McGregor et al.
Kidney international, 59(6), 2267-2272 (2001-05-31)
Hyperhomocysteinemia is a risk factor for atherosclerosis that is common in chronic renal failure (CRF), but its cause is unknown. Homocysteine metabolism is linked to betaine-homocysteine methyl transferase (BHMT), a zinc metalloenzyme that converts glycine betaine (GB) to N,N dimethylglycine
Christine App et al.
Annals of the New York Academy of Sciences, 1270, 98-104 (2012-10-12)
In this review, we identify potential interaction partners of the β-thymosin family. The proteins of this family are highly conserved peptides in mammals and yet only one intracellular (G-actin) and one cell-surface protein (β subunit of F(1) -F(0) ATP synthase)
Jiwoong Kwon et al.
Scientific reports, 5, 17804-17804 (2015-12-08)
Far-field optical nanoscopy has been widely used to image small objects with sub-diffraction-limit spatial resolution. Particularly, reversible saturable optical fluorescence transition (RESOLFT) nanoscopy with photoswitchable fluorescent proteins is a powerful method for super-resolution imaging of living cells with low light
Philip R Williams et al.
Nature communications, 5, 5683-5683 (2014-12-17)
Therapeutic strategies for spinal cord injury (SCI) commonly focus on regenerating disconnected axons. An alternative approach would be to maintain continuity of damaged axons, especially after contusion. The viability of such neuropreservative strategies depends on the degree to which initially
Han Ting Chou et al.
Journal of bacteriology, 195(17), 3906-3913 (2013-06-25)
Pseudomonas aeruginosa PAO1 grows on a variety of polyamines as the sole source of carbon and nitrogen. Catabolism of polyamines is mediated by the γ-glutamylation pathway, which is complicated by the existence of multiple homologous enzymes with redundant specificities toward
Kenichi Shibuya et al.
Journal of experimental botany, 64(4), 1111-1120 (2013-01-26)
Autophagy is one of the main mechanisms of degradation and remobilization of macromolecules, and it appears to play an important role in petal senescence. However, little is known about the regulatory mechanisms of autophagy in petal senescence. Autophagic processes were
D S Gill et al.
Journal of clinical pathology, 44(3), 243-245 (1991-03-01)
Plasma histamine concentrations were measured using a commercially available monoclonal antibody radioimmunoassay in 38 patients with nephrotic syndrome, end stage renal failure, those receiving haemodialysis, and those receiving continuous ambulatory peritoneal dialysis to determine whether histamine may mediate damage to
K Becker et al.
Experimental dermatology, 12(3), 324-329 (2003-06-26)
Congenital ichthyosiform erythroderma (CIE) belongs together with lamellar ichthyosis (LI) to the group of autosomal recessive congenital ichthyoses (ARCI). Mutations in the transglutaminase (TGase) 1 gene (TGM1) have been identified in several families with LI and in some families with
Ji Young Kim et al.
Archives of oral biology, 57(8), 1018-1025 (2012-05-05)
Curcumin, a major active component of turmeric Curcuma longa, has been shown to have inhibitory effects on cancers. In vitro studies suggest that curcumin inhibits cancer cell growth by activating apoptosis, but the mechanism underlying the anticancer effects of curcumin
Maria Rosecler Miranda Rossetto et al.
Food chemistry, 188, 218-224 (2015-06-05)
Relationships between endogenous levels of polyamines by thin layer chromatography (TLC) and gas chromatography (GC), nitrate and response to the application of ethylene were established between organic and conventional vegetables (broccoli, collard greens, carrots and beets), both raw and cooked.
Wei Ye et al.
Connective tissue research, 54(1), 22-28 (2012-07-28)
Macroautophagy increases with age in rat intervertebral discs; however, the effect of macroautophagy on the process of intervertebral disc degeneration (IVDD) is still unclear. The aim of this study was to examine the presence of autophagosome, as well as the
Christine Frederickx et al.
Journal of forensic sciences, 59(1), 242-249 (2013-08-29)
This study investigated the role of odorant cues used during host-habitat location by the generalist parasitoid, Nasonia vitripennis Walker. Nasonia vitripennis is a common parasitoid of Dipteran pupae found in association with decaying carrion. Behavioral assays were used to investigate
Yanyan Wang et al.
Cellular immunology, 274(1-2), 121-129 (2012-02-22)
Recognition of lipopolysaccharide (LPS) by Toll-like receptor 4 (TLR4) activates two district proinflammatory signaling pathway and initiates LPS internalization. To investigate roles of LPS internalization, a traditionally regarded metabolic pathway for LPS, in regulation of these two pathways, three internalization
T Sakai et al.
Clinical chemistry, 36(3), 474-476 (1990-03-01)
We devised a kit for use with automated analyzers, for assay of urinary free L-fucose by means of a newly isolated L-fucose dehydrogenase (EC, and we measured L-fucose in healthy subjects, cancer patients, and patients with other diseases. It
Ashiq Hussain et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(48), 19579-19584 (2013-11-13)
Carrion smell is strongly repugnant to humans and triggers distinct innate behaviors in many other species. This smell is mainly carried by two small aliphatic diamines, putrescine and cadaverine, which are generated by bacterial decarboxylation of the basic amino acids
Anne M Scott et al.
PLoS biology, 17(7), e3000332-e3000332 (2019-07-10)
Semen is fundamental for sexual reproduction. The non-sperm part of ejaculated semen, or seminal plasma, facilitates the delivery of sperm to the eggs. The seminal plasma of some species with internal fertilization contains anti-aphrodisiac molecules that deter promiscuity in post-copulatory
Erin E Congdon et al.
The Journal of biological chemistry, 288(49), 35452-35465 (2013-10-29)
Tau immunotherapy is effective in transgenic mice, but the mechanisms of Tau clearance are not well known. To this end, Tau antibody uptake was analyzed in brain slice cultures and primary neurons. Internalization was rapid (<1 h), saturable, and substantial
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