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Shaodong Jia et al.
Journal of chromatography. A, 1218(51), 9174-9182 (2011-11-15)
A novel liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (LC-Q-TOFMS) method was developed for the simultaneous determination of 23 amino acids and 7 biogenic amines in food samples. These analytes were pre-column derivatized with dansyl chloride and then separated...
Ming-Hua Liang et al.
Journal of the science of food and agriculture, 95(9), 1949-1955 (2014-09-11)
To decrease the methanol content of the sugar cane sprits, mutagenesis of ultraviolet (UV) coupled with diethyl sulfate (DES) was used to generate a mutant of Saccharomyces cerevisiae with lower methanol content. Meanwhile, the effects of the additions of pectinase...
Maria T Hultman et al.
Aquatic toxicology (Amsterdam, Netherlands), 159, 233-244 (2015-01-07)
Vitellogenin (Vtg) induction in primary fish hepatocytes has been proposed as an in vitro screening assay for ER agonists and antagonists, but has not yet been used extensively as a high-throughput screening tool due to poor reproducibility, sensitivity and overall...
DeQing Cao et al.
Drug and chemical toxicology, 38(1), 44-49 (2014-03-22)
A Type II pyrethroid pesticide β-cypermethrin is widely used in agriculture and domestic applications for pest control. However, the effect of β-cypermethrin on the glutamate neurotransmitter has not been well-documented. In the current study, mice were treated with 20, 40...
Juan C Marini
Rapid communications in mass spectrometry : RCM, 25(9), 1291-1296 (2011-04-15)
The enteral metabolisms of glutamine and citrulline are intertwined because, while glutamine is one of the main fuel sources for the enterocyte, citrulline is one of its products. It has been shown that the administration of (15)N-labeled glutamine results in...
Hong Chang et al.
Environmental pollution (Barking, Essex : 1987), 235, 881-888 (2018-01-23)
An isotope-dilution ultra-performance liquid chromatography-electrospray tandem mass spectrometry method combined with dansylation was established to sensitively quantify four steroid estrogens (estrone, 17α-estradiol, 17β-estradiol and 17α-ethynylestradiol) and bisphenol A in sewage influent and effluent. A simple hexane extraction was performed from...
Zeena S Pillai et al.
Chemistry, an Asian journal, 8(4), 771-777 (2013-01-25)
We have designed two novel dendrimers with cyclam cores with appended poly(amido amine) (PAMAM) dendrons, decorated at the periphery with four and eight dansyl chromophores, respectively. The photophysical properties of the dendrimers and their Nd(3+) complexes have been investigated. The...
Arisa Yamada et al.
Biomacromolecules, 16(8), 2356-2362 (2015-06-30)
We report the development of environmentally responsive fluorescent polymers. The reversible temperature-induced phase transition of copolymers composed of N-isopropylacrylamide and a fluorescent monomer based on the fluorescein (FL), coumarin (CO), rhodamine (RH), or dansyl (DA) skeleton was used as a...
Miguel López-Gómez et al.
Phytochemistry, 107, 32-41 (2014-09-16)
Polyamines (PAs) are low molecular weight aliphatic compounds that have been shown to be an important part of plant responses to salt stress. For that reason in this work we have investigated the involvement of PAs in the response to...
Sarah A Goretta et al.
Bioorganic & medicinal chemistry, 20(13), 4012-4019 (2012-06-08)
Sphingomyelin (SM) and cholesterol form microdomains called lipid rafts in cellular membranes. To develop a versatile fluorescent lipid probe, chemical modifications to both the hydrophobic and hydrophilic portions of SM are essential. Few reports describing SM probes with a fluorophore...
Balaraman H Shankar et al.
The journal of physical chemistry. B, 115(45), 13292-13299 (2011-10-11)
Interaction of a few dansyl-naphthalimide conjugates 1a-e linked through polymethylene spacer groups with various metal ions was investigated through absorption, fluorescence, NMR, isothermal calorimetric (ITC), and laser flash photolysis techniques. The characteristic feature of these dyads is that they exhibit...
The use of dansyl-chloride for the detection of amino acids and serotonin in nervous tissue
Research Methods in Neurochemistry, 443-462 (1975)
Xingnan Li et al.
Steroids, 99(Pt A), 84-90 (2014-12-30)
Estrogen metabolites are important biomarkers to evaluate cancer risks and metabolic diseases. Due to their low physiological levels, a sensitive and accurate method is required, especially for the quantitation of unconjugated forms of endogenous steroids and their metabolites in humans....
Ramakrishna Nirogi et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 913-914, 41-47 (2012-12-29)
A rapid and sensitive liquid chromatography tandem mass spectrometry method for simultaneous quantification of catecholamine neurotransmitters in microdialysates was developed. The catecholamine neurotransmitters dopamine (DA) and norepinephrine (NE) were pre-column derivatized with dansyl chloride and analyzed. A gradient elution method...
Zhongmei Tang et al.
Analytical chemistry, 82(18), 7706-7712 (2010-08-28)
Chemical derivatization is useful for improving the ionization characteristics of poorly or nonionizable analytes in liquid chromatography-mass spectrometry (LC-MS). Dansyl chloride has been widely used as a derivatizing reagent for fluorescence detection and for facilitating the MS detection of phenols...
Audrey Roy-Lachapelle et al.
Analytical and bioanalytical chemistry, 407(18), 5487-5501 (2015-05-04)
A new analytical method was developed for the detection of alkaloid cyanotoxins in harmful algal blooms. The detection of the nonproteinogenic amino acid β-N-methylamino-L-alanine (BMAA) and two of its conformation isomers, 2,4-diaminobutyric acid (DAB) and N-(2-aminoethyl) glycine (AEG), as well...
E Bichon et al.
Rapid communications in mass spectrometry : RCM, 26(7), 819-827 (2012-03-01)
The control of forbidden anabolic practices in cattle in the European Union has become challenging since endogenous compounds such as estradiol derivatives can potentially be used as growth promoters. Due to the great difficulty in establishing a reference threshold value...
Didier Blanot et al.
Chemical biology & drug design, 79(1), 2-8 (2011-08-06)
Muramyl peptides derived from bacterial peptidoglycan have long been known for their ability to trigger host innate immune responses, including inflammation and antimicrobial defense. Muramyl peptides have also been widely studied for their role as immune adjuvants. In mammals, the...
Yuyin Zhou et al.
Environmental science & technology, 49(12), 7218-7226 (2015-05-23)
The occurrence of bisphenol A (BPA), nonylphenol (NP), and their six chlorinated byproducts were investigated in 74 food contacting papers (FCPs) from China, the U.S.A., Japan, and Europe using a sensitive dansylation LC-MS/MS method. BPA (<LOQ-83 ng/g) and NP (<LOQ-5.4...
Giovanni Licari et al.
Xenobiotica; the fate of foreign compounds in biological systems, 45(1), 3-9 (2014-07-23)
1. Perhexiline, a chiral anti-anginal agent, may be useful to develop new cardiovascular therapies, despite its potential hepatotoxicity. 2. This study compared Dark Agouti (DA) and Sprague-Dawley (SD) rats, as models of perhexiline's metabolism and hepatotoxicity in humans. Rats (n...
Radia Bousoumah et al.
Analytical and bioanalytical chemistry, 407(29), 8713-8723 (2015-09-24)
Multi-residue methods permitting the high-throughput and affordable simultaneous determination of an extended range of endocrine disrupting chemicals (EDCs) with reduced time and cost of analysis is of prime interest in order to characterize a whole set of bioactive compounds. Such a...
Antonis Myridakis et al.
Analytical and bioanalytical chemistry, 407(9), 2509-2518 (2015-02-04)
Phthalates, bisphenol A (BPA) and parabens (PBs), organic chemicals widely used in everyday products, are considered to be endocrine disruptors. We propose a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of seven phthalate metabolites, six PBs and BPA...
Covalent protein immobilization on glass surfaces: Application to alkaline phosphatase
Taylor RH, et al.
Journal of Biotechnology, 118(3), 265-269 (2005)
Jérémy Carlier et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 978-979, 70-77 (2014-12-23)
Hypoglycin A (HGA) is the toxic principle in ackee (Blighia sapida Koenig), a nutritious and readily available fruit which is a staple of the Jamaican working-class and rural population. The aril of the unripe fruit has high concentrations of HGA...
M Lisa Manier et al.
Journal of mass spectrometry : JMS, 49(8), 665-673 (2014-07-22)
Imaging mass spectrometry (IMS) studies increasingly focus on endogenous small molecular weight metabolites and consequently bring special analytical challenges. Since analytical tissue blanks do not exist for endogenous metabolites, careful consideration must be given to confirm molecular identity. Here, we...
Tadeu Chaves de Figueiredo et al.
Talanta, 142, 240-245 (2015-05-25)
A quantitative and confirmatory high-performance liquid chromatography with ultraviolet detection (HPLC-UV) method for the determination of bioactive amines in the albumen and yolk of commercial eggs was developed, optimized and validated by analyte extraction with trichloroacetic acid and pre-column derivatization...
Measuring the poise of thiol/disulfide couples in vivo
Jones DP and Liang Y
Free radical biology & medicine, 47(10), 1329-1338 (2009)
Ricardo Souza Reis et al.
Journal of proteomics, 130, 170-179 (2015-10-06)
Somatic embryogenesis, an important biotechnological technique, has great potential for application in sugarcane breeding and micropropagation. Polyamines have been associated with the regulation of several physiological processes, including the acquisition of embryogenic competence and somatic embryogenesis. In this study, we...
Hien P Nguyen et al.
Journal of pharmaceutical and biomedical analysis, 54(4), 830-837 (2010-12-15)
Estrogens are known to exhibit neuroprotective effects on the brain. Their importance in this regard and in others has been emphasized in many recent studies, which increases the need to develop reliable analytical methods for the measurement of estrogen hormones....
Xiaomei Zhuang et al.
Journal of pharmaceutical and biomedical analysis, 75, 18-24 (2013-01-15)
Psoralea corylifolia L. (Fabaceae) is a traditional Chinese medicine with many beneficial effects in medical therapies. Bakuchiol was the main active ingredient of Psoralea corylifolia L. In this study, a novel method of pre-column derivatization with dansyl chloride followed by...
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