Showing 1-30 of 38 results for "D2886"
Chen Wang et al.
International journal of radiation oncology, biology, physics, 86(3), 440-449 (2013-02-26)
DNA damage can occur as a result of endogenous metabolic reactions and replication stress or from exogenous sources such as radiation therapy and chemotherapy. DNA double strand breaks are the most cytotoxic form of DNA damage, and defects in their...
The enzymatic repair of DNA, II. Characterization of phage-induced sealase.
A Becker et al.
Proceedings of the National Academy of Sciences of the United States of America, 58(5), 1996-2003 (1967-11-01)
Jingyin Yue et al.
PloS one, 8(1), e54389-e54389 (2013-02-02)
Dubowitz Syndrome is an autosomal recessive disorder with a unique set of clinical features including microcephaly and susceptibility to tumor formation. Although more than 140 cases of Dubowitz syndrome have been reported since 1965, the genetic defects of this disease...
Engler, M.J. and Richardson, C.C. et al.
The Enzymes, 5, 3-3 (1982)
M J Moore et al.
Science (New York, N.Y.), 256(5059), 992-997 (1992-05-15)
A simple and efficient method for synthesizing long, site-specifically modified RNA molecules was developed whereby segments of RNA were joined with the use of bacteriophage T4 DNA ligase. A single hydrogen or O-methyl group was substituted for the 2'-hydroxyl group...
Hiroshi Ochiai et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(27), 10915-10920 (2012-06-20)
To understand complex biological systems, such as the development of multicellular organisms, it is important to characterize the gene expression dynamics. However, there is currently no universal technique for targeted insertion of reporter genes and quantitative imaging in multicellular model...
Sehyun Oh et al.
Nucleic acids research, 41(3), 1734-1749 (2013-01-01)
Classic non-homologous end joining (C-NHEJ) is the predominant DNA double-strand break repair pathway in humans. Although seven genes Ku70, Ku86, DNA-PK(cs), Artemis, DNA Ligase IV (LIGIV), X-ray cross-complementing group 4 and XRCC4-like factor are required for C-NHEJ, several of them...
Xin Cheng et al.
European journal of cell biology, 91(10), 782-788 (2012-08-04)
Translocation of mitochondrial DNA (mtDNA) fragments to the nucleus and insertion of those fragments into nuclear DNA has been observed in several organisms ranging from yeast to plants and mammals. Disruption of specific nuclear genes by de novo insertions of...
Wenxin Gu et al.
Bioorganic & medicinal chemistry letters, 22(11), 3693-3698 (2012-05-09)
A series of 2,6-disubstituted aminoalkoxypyrimidine carboxamides (AAPCs) with potent inhibition of bacterial NAD(+)-dependent DNA ligase was discovered through the use of structure-guided design. Two subsites in the NAD(+)-binding pocket were explored to modulate enzyme inhibitory potency: a hydrophobic selectivity region...
Enzymatic breakage and joining of deoxyribonucleic acid, I. Repair of single-strand breaks in DNA by an enzyme system from Escherichia coli infected with T4 bacteriophage.
B Weiss et al.
Proceedings of the National Academy of Sciences of the United States of America, 57(4), 1021-1028 (1967-04-01)
J R Rusche et al.
Nucleic acids research, 13(6), 1997-2008 (1985-03-25)
Hexamine cobalt chloride (HCC) increases the efficiency of blunt end ligation by T4 DNA ligase about 50 fold. Maximum stimulation occurs when standard buffers for ligation are supplemented with 1 mM HCC. All the ligation events are intermolecular regardless of...
S Kaur
Experimental oncology, 35(3), 226-228 (2013-10-03)
DNA repair genetic polymorphisms may affect cancer susceptibility as genetic variations in DNA repair genes may influence DNA repair capacity. In the present study, the association of polymorphic forms of DNA repair gene, DNA ligase I (LIGI) was examined with...
Dana Vigasova et al.
Nucleic acids research, 41(10), 5341-5353 (2013-04-11)
Non-homologous end-joining (NHEJ) repairs DNA double-strand breaks by tethering and ligating the two DNA ends. The mechanisms regulating NHEJ efficiency and interplay between its components are not fully understood. Here, we identify and characterize the SUMOylation of budding yeast Lif1...
T Petrova et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 68(Pt 12), 1440-1447 (2012-11-30)
DNA ligases join single-strand breaks in double-stranded DNA by catalyzing the formation of a phosphodiester bond between adjacent 5'-phosphate and 3'-hydroxyl termini. Their function is essential for maintaining genome integrity in the replication, recombination and repair of DNA. High flexibility...
Akira Sassa et al.
The Journal of biological chemistry, 287(44), 36702-36710 (2012-09-20)
Human 8-oxoguanine DNA glycosylase (OGG1) is a key enzyme involved in removing 7,8-dihydro-8-oxoguanine (8-oxoG), a highly mutagenic DNA lesion generated by oxidative stress. The removal of 8-oxoG by OGG1 is affected by the local DNA sequence, and this feature most...
Feng Chen et al.
Biosensors & bioelectronics, 47, 218-224 (2013-04-16)
DNA phosphorylation, catalyzed by polynucleotide kinase (PNK), plays significant regulatory roles in many biological events. Herein, using T4 PNK as a model target, we describe a one-step, highly sensitive, simple and rapid fluorescence approach for monitoring its activity and inhibition....
Adam B Robertson et al.
The Journal of biological chemistry, 287(39), 32953-32966 (2012-08-01)
The Escherichia coli very short patch (VSP) repair pathway corrects thymidine-guanine mismatches that result from spontaneous hydrolytic deamination damage of 5-methyl cytosine. The VSP repair pathway requires the Vsr endonuclease, DNA polymerase I, a DNA ligase, MutS, and MutL to...
Athena Kantartzis et al.
Cell reports, 2(2), 216-222 (2012-09-04)
Trinucleotide repeat (TNR) expansions are the underlying cause of more than 40 neurodegenerative and neuromuscular diseases, including myotonic dystrophy and Huntington's disease. Although genetic evidence points to errors in DNA replication and/or repair as the cause of these diseases, clear...
Justin L Sparks et al.
Molecular cell, 47(6), 980-986 (2012-08-07)
Ribonucleotides are incorporated into DNA by the replicative DNA polymerases at frequencies of about 2 per kb, which makes them by far the most abundant form of potential DNA damage in the cell. Their removal is essential for restoring a...
Pablo De Ioannes et al.
Cell reports, 2(6), 1505-1512 (2012-12-12)
DNA ligase IV (LigIV) and Artemis are central components of the nonhomologous end-joining (NHEJ) machinery that is required for V(D)J recombination and the maintenance of genomic integrity in mammalian cells. We report here crystal structures of the LigIV DNA binding...
Sicheng Liu et al.
Biochemical and biophysical research communications, 439(2), 173-178 (2013-09-03)
DNA ligase IV (LIG4) and XRCC4 form a complex to ligate two DNA ends at the final step of DNA double-strand break (DSB) repair through non-homologous end-joining (NHEJ). It is not fully understood how these proteins are recruited to DSBs....
Yi Zhou et al.
The Journal of biological chemistry, 288(52), 37112-37125 (2013-11-14)
The resection of DNA double strand breaks initiates homologous recombination (HR) and is critical for genomic stability. Using direct measurement of resection in human cells and reconstituted assays of resection with purified proteins in vitro, we show that DNA-dependent protein...
Takashi Ochi et al.
Structure (London, England : 1993), 20(7), 1212-1222 (2012-06-05)
Knowledge of the architecture of DNA ligase IV (LigIV) and interactions with XRCC4 and XLF-Cernunnos is necessary for understanding its role in the ligation of double-strand breaks during nonhomologous end joining. Here we report the structure of a subdomain of...
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 1-1 (1989)
Jessica Cottarel et al.
The Journal of cell biology, 200(2), 173-186 (2013-01-23)
Nonhomologous end joining is the primary deoxyribonucleic acid (DNA) double-strand break repair pathway in multicellular eukaryotes. To initiate repair, Ku binds DNA ends and recruits the DNA-dependent protein kinase (DNA-PK) catalytic subunit (DNA-PKcs) forming the holoenzyme. Early end synapsis is...
Ayako Nishizawa-Yokoi et al.
The New phytologist, 196(4), 1048-1059 (2012-10-12)
Evidence for the involvement of the nonhomologous end joining (NHEJ) pathway in Agrobacterium-mediated transferred DNA (T-DNA) integration into the genome of the model plant Arabidopsis remains inconclusive. Having established a rapid and highly efficient Agrobacterium-mediated transformation system in rice (Oryza...
Timra Gilson et al.
Virology, 428(2), 128-135 (2012-04-27)
In adenovirus E4 mutant infections, viral DNAs form concatemers through a process that requires host Non-homologous End Joining (NHEJ) proteins including DNA Ligase IV (LigIV). Adenovirus proteins E4 34k and E1b 55k form the substrate-selection component of an E3 ubiquitin...
Kinetic characterization of single strand break ligation in duplex DNA by T4 DNA ligase.
Lohman GJ
The Journal of Biological Chemistry, 286(51), 44187-44196 (2011)
Hui Zhu et al.
Biochemistry, 51(51), 10147-10158 (2012-12-04)
Mycobacteria exploit nonhomologous end-joining (NHEJ) to repair DNA double-strand breaks. The core NHEJ machinery comprises the homodimeric DNA end-binding protein Ku and DNA ligase D (LigD), a modular enzyme composed of a C-terminal ATP-dependent ligase domain (LIG), a central 3'-phosphoesterase...
Purification and properties of a polynucleotide ligase from calf thymus glands.
U Bertazzoni et al.
Biochimica et biophysica acta, 287(3), 404-414 (1972-12-22)

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