Showing 1-30 of 258 results for "

D6429

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Jonatan Dorca-Arévalo et al.
Veterinary research, 51(1), 27-27 (2020-02-26)
The pore-forming protein epsilon toxin (Etx) from Clostridium perfringens produces acute perivascular edema affecting several organs, especially the brain and lungs. Despite the toxin evident effect on microvasculature and endothelial cells, the underlying molecular and cellular mechanisms remain obscure. Moreover...
Xiaolu Zhang et al.
Nature communications, 10(1), 4732-4732 (2019-10-20)
Current multiplexing strategies for massively parallel sequencing of genomic DNA mainly rely on library indexing in the final steps of library preparation. This procedure is costly and time-consuming, because a library must be generated separately for each sample. Furthermore, library...
Hirohiko Niioka et al.
Human cell, 31(1), 87-93 (2017-12-14)
In the field of regenerative medicine, tremendous numbers of cells are necessary for tissue/organ regeneration. Today automatic cell-culturing system has been developed. The next step is constructing a non-invasive method to monitor the conditions of cells automatically. As an image...
Suman Mukhopadhyay et al.
Cell cycle (Georgetown, Tex.), 14(20), 3331-3339 (2015-09-01)
mTOR - the mammalian/mechanistic target of rapamycin - has been implicated as a key signaling node for promoting survival of cancer cells. However, clinical trials that have targeted mTOR with rapamycin or rapamycin analogs have had minimal impact. In spite...
Paridhi Agrawal et al.
ACS omega, 5(33), 20817-20824 (2020-09-03)
Long-read genomics technologies such as nanopore sequencing and genome mapping in nanochannels extract genomic information in the kilobase to megabase pair range from single DNA molecules, thereby overcoming read-length limitations in next-generation DNA sequencing. Long-read technologies start with long DNA...
Waldemar Schrimpf et al.
Methods (San Diego, Calif.), 140-141, 97-111 (2018-02-07)
Raster image cross-correlation spectroscopy (ccRICS) can be used to quantify the interaction affinities between diffusing molecules by analyzing the fluctuations between two-color confocal images. Spectral crosstalk compromises the quantitative analysis of ccRICS experiments, limiting multicolor implementations to dyes with well-separated...
Balamurugan Packialakshmi et al.
PloS one, 13(4), e0196277-e0196277 (2018-04-25)
Increases of the activity of mitochondrial electron transport chain generally lead to increases of production of ATP and reactive oxygen species (ROS) as by-products. MnSOD is the first line of defense against the stress induced by mitochondrial ROS. Our previous...
Gustavo Duque et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 35(11), 2275-2288 (2020-07-07)
Fractures attributable to osteoporosis have a severe impact on our older population. Reports of side effects with commonly prescribed osteoporosis drugs have led to the investigation of new and safer treatments with novel mechanisms of action. Picolinic acid (PIC), a...
Kelsey N Lamb et al.
Cell chemical biology, 26(10), 1365-1379 (2019-08-20)
Polycomb-directed repression of gene expression is frequently misregulated in human diseases. A quantitative and target-specific cellular assay was utilized to discover the first potent positive allosteric modulator (PAM) peptidomimetic, UNC4976, of nucleic acid binding by CBX7, a chromodomain methyl-lysine reader...
Clémentine Lesbats et al.
NMR in biomedicine, 33(11), e4386-e4386 (2020-07-31)
The utility of diffusion kurtosis imaging (DKI) for assessing intra-tumor heterogeneity was evaluated in a rat model of glioblastoma multiforme. Longitudinal MRI including T2 -weighted and diffusion-weighted MRI (DWI) was performed on six female Fischer rats 8, 11 and 14...
Anna Pepe et al.
Scientific reports, 7(1), 3731-3731 (2017-06-18)
Shadoo (Sho), a member of prion protein family, has been shown to prevent embryonic lethality in Prnp
Hyemin Lee et al.
Nature cell biology, 22(2), 225-234 (2020-02-08)
Energy stress depletes ATP and induces cell death. Here we identify an unexpected inhibitory role of energy stress on ferroptosis, a form of regulated cell death induced by iron-dependent lipid peroxidation. We found that ferroptotic cell death and lipid peroxidation...
Taha Azad et al.
Oncogene, 39(2), 334-355 (2019-09-04)
The Hippo pathway has emerged as a key signaling pathway that regulates various biological functions. Dysregulation of the Hippo pathway has been implicated in a broad range of human cancer types. While a number of stimuli affecting the Hippo pathway...
Digamber Rane et al.
Methods in enzymology, 640, 1-35 (2020-06-21)
Peroxynitrite (PNT) is a highly reactive oxidant that plays a key role in the destruction of foreign pathogens by specific phagocytic immune cells such as macrophages. However, when its production is dysregulated, this oxidant can contribute to cardiovascular disease, neurological...
Antaripa Bhattacharya et al.
Journal of personalized medicine, 10(4) (2020-11-20)
Alzheimer's disease (AD) is a fatal neurodegenerative disorder caused by protein misfolding and aggregation, affecting brain function and causing dementia. Amyloid beta (Aβ), a peptide deriving from amyloid precursor protein (APP) cleavage by-and γ-secretases, is considered a pathological hallmark of...
Paula A Hernandez et al.
JOR spine, 3(4), e1106-e1106 (2021-01-05)
The interaction between cells and their extracellular matrix (ECM) is crucial to maintain both tissue and cellular homeostasis. Indeed, cell phenotype is significantly affected by the 3D microenvironment. Although highly convenient, isolating cells from the intervertebral disc (IVD) and growing...
Teodor E Yordanov et al.
Traffic (Copenhagen, Denmark), 20(9), 674-696 (2019-07-18)
Mechanisms that control lysosomal function are essential for cellular homeostasis. Lysosomes adapt in size and number to cellular needs but little is known about the underlying molecular mechanism. We demonstrate that the late endosomal/lysosomal multimeric BLOC-1-related complex (BORC) regulates the...
Susan Wagner et al.
Nucleic acids research, 44(22), 10772-10788 (2016-12-08)
The 12-subunit mammalian eIF3 is the largest and most complex translation initiation factor and has been implicated in numerous steps of translation initiation, termination and ribosomal recycling. Imbalanced eIF3 expression levels are observed in various types of cancer and developmental...
Anna Herrmannová et al.
Nucleic acids research, 48(4), 1969-1984 (2019-12-22)
One of the key roles of the 12-subunit eukaryotic translation initiation factor 3 (eIF3) is to promote the formation of the 43S and 48S pre-initiation complexes (PICs). However, particular contributions of its individual subunits to these two critical initiation reactions...
Jessica E Miller et al.
Scientific reports, 7(1), 17903-17903 (2017-12-22)
Endometriosis is a debilitating condition that is categorized by the abnormal growth of endometrial tissue outside the uterus. Although the pathogenesis of this disease remains unknown, it is well established that endometriosis patients exhibit immune dysfunction. Interleukin (IL)-33 is a...
Jacqueline D Peda et al.
Disease models & mechanisms, 9(9), 1051-1061 (2016-08-05)
Polycystic kidney disease (PKD) is characterized by slow expansion of fluid-filled cysts derived from tubules within the kidney. Cystic expansion results in injury to surrounding parenchyma and leads to inflammation, scarring and ultimately loss of renal function. Macrophages are a...
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Frank Bunge et al.
Biomedical microdevices, 20(4), 98-98 (2018-11-11)
Microfluidic cell cultures are often used in academic research but only rarely in pharmaceutical research because of unsuitable designs, inappropriate choice of materials or incompatibility with standard equipment. In particular, microfluidic cell cultures to control the gaseous microenvironment rely on...
Grace G Bushnell et al.
Cancer research, 79(8), 2042-2053 (2019-02-28)
For most cancers, metastasis is the point at which clinical treatment shifts from curative intent to extending survival. Biomaterial implants acting as a synthetic premetastatic niche recruit metastatic cancer cells and provide a survival advantage, and their use as a...
Joao Fonseca et al.
Oncology letters, 17(1), 697-705 (2019-01-19)
Resveratrol (RES) is a polyphenol produced by certain plant species that has been well studied due to its ability to slow the growth of cancer cells. In numerous cell types and tissues, RES has been demonstrated to promote mitochondrial biogenesis...
Zhenqiang Sun et al.
Journal of Cancer, 12(9), 2723-2734 (2021-04-16)
Purpose: Tumor metastasis seriously affects the survival of patients. In recent years, some studies confirmed that long non-coding RNA (lncRNA) played an essential role in tumor progression. A few studies reported that LINC01296 acted as an oncogenic regulator of cancer....
Toshimichi Tanaka et al.
Annals of surgical oncology, 26(5), 1401-1411 (2019-02-02)
Treatment-resistance genes limiting anticancer therapy have not been well clarified in colorectal cancer (CRC). We explored gene expression profiles to identify biomarkers for predicting treatment resistance to an anticancer drug in CRC. Six CRC cell lines were treated with phenylbutyrate...
Kazuhiro Ikeda et al.
Scientific reports, 7(1), 2850-2850 (2017-06-08)
Human pluripotent stem cells are a potentially powerful cellular resource for application in regenerative medicine. Because such applications require large numbers of human pluripotent stem cell-derived cells, a scalable culture system of human pluripotent stem cell needs to be developed....
Douglas K Peters et al.
PLoS pathogens, 16(3), e1008403-e1008403 (2020-03-24)
The replication of small DNA viruses requires both host DNA replication and repair factors that are often recruited to subnuclear domains termed viral replication centers (VRCs). Aside from serving as a spatial focus for viral replication, little is known about...
Alejandro Carnicer-Lombarte et al.
Journal of neural engineering, 14(3), 036012-036012 (2017-03-09)
High-density electrode arrays are a powerful tool in both clinical neuroscience and basic research. However, current manufacturing techniques require the use of specialised techniques and equipment, which are available to few labs. We have developed a high-density electrode array with...