Showing 1-30 of 131 results for "

D6546

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Haruka Chino et al.
Molecular cell, 74(5), 909-921 (2019-04-23)
Certain proteins and organelles can be selectively degraded by autophagy. Typical substrates and receptors of selective autophagy have LC3-interacting regions (LIRs) that bind to autophagosomal LC3 and GABARAP family proteins. Here, we performed a differential interactome screen using wild-type LC3B...
Tobias H Dovmark et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 32(1), 83-96 (2017-09-09)
ATP fuels the removal of metabolic end-products, including H
Geraldine M Flaujac Lafontaine et al.
Nutrients, 12(5) (2020-05-06)
Prebiotic oligosaccharides are widely used as human and animal feed additives for their beneficial effects on the gut microbiota. However, there are limited data to assess the direct effect of such functional foods on the transcriptome of intestinal epithelial cells....
S Martin et al.
Biochemical pharmacology, 93(3), 290-304 (2014-12-23)
Vemurafenib (PLX4032), an inhibitor of BRAF(V600E), has demonstrated significant clinical anti-melanoma effects. However, the majority of treated patients develop resistance, due to a variety of molecular mechanisms including MAPK reactivation through MEK. The induction of a cancer cell death modality...
Tomohiro Furukawa et al.
Cell chemical biology, 27(11), 1396-1409 (2020-09-06)
Aflatoxin contamination of crops is a serious problem worldwide. Utilization of aflatoxin production inhibitors is attractive, as the elucidation of their modes of action contributes to clarifying the mechanism of aflatoxin production. Here, we identified mitochondrial protease ClpP as the...
Janja Dermol-Černe et al.
Scientific reports, 10(1), 9149-9149 (2020-06-06)
In gene electrotransfer and cardiac ablation with irreversible electroporation, treated muscle cells are typically of elongated shape and their orientation may vary. Orientation of cells in electric field has been reported to affect electroporation, and hence electrodes placement and pulse...
Veena Padmanaban et al.
Nature, 573(7774), 439-444 (2019-09-06)
Metastasis is the major driver of death in patients with cancer. Invasion of surrounding tissues and metastasis have been proposed to initiate following loss of the intercellular adhesion protein, E-cadherin1,2, on the basis of inverse correlations between in vitro migration...
Kevin Moreau et al.
Nature communications, 6, 8045-8045 (2015-08-21)
Autophagy is an important degradation pathway, which is induced after starvation, where it buffers nutrient deprivation by recycling macromolecules in organisms from yeast to man. While the classical pathway mediating this response is via mTOR inhibition, there are likely to...
Marco Diociaiuti et al.
Biomolecules, 10(1) (2020-01-08)
To investigate the interaction between amyloid assemblies and "lipid-rafts", we performed functional and structural experiments on salmon calcitonin (sCT) solutions rich in prefibrillar oligomers, proto- and mature-fibers interacting with liposomes made of monosialoganglioside-GM1 (4%), DPPC (48%) and cholesterol (48%). To...
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Dan N Simon et al.
Cells, 7(5) (2018-05-19)
The LMNA gene encodes lamins A and C with key roles in nuclear structure, signaling, gene regulation, and genome integrity. Mutations in LMNA cause over 12 diseases ('laminopathies'). Lamins A and C are identical for their first 566 residues. However...
Nadia Nabil Haj-Yasein et al.
Biochimica et biophysica acta, 1862(6), 623-635 (2017-02-22)
Plasma cysteine is strongly associated with body fat mass in human cohorts and diets low in cysteine prevents fat accumulation in mice. It is unclear if plasma cysteine affects fat development or if fat accumulation raises plasma cysteine. To determine...
Elliot D Mock et al.
Nature chemical biology, 16(6), 667-675 (2020-05-13)
N-acylethanolamines (NAEs), which include the endocannabinoid anandamide, represent an important family of signaling lipids in the brain. The lack of chemical probes that modulate NAE biosynthesis in living systems hamper the understanding of the biological role of these lipids. Using...
Michael P Greenwood et al.
Molecular brain, 9, 1-1 (2016-01-08)
Rasd1 is a member of the Ras family of monomeric G proteins that was first identified as a dexamethasone inducible gene in the pituitary corticotroph cell line AtT20. Using microarrays we previously identified increased Rasd1 mRNA expression in the rat...
Akitoshi Inoue et al.
STAR protocols, 1(3), 100166-100166 (2020-12-31)
This protocol describes BATTLE-1EX, which is a combined method of BATTLE-1 and expansion microscopy to obtain high-resolution imaging of whole synaptic structures and their components of hippocampal neural circuits. BATTLE-1 uses two genetically engineered recombinase proteins and competition between two...
Miles R Bryan et al.
Human molecular genetics, 28(22), 3825-3841 (2019-10-11)
The molecular etiology linking the pathogenic mutations in the Huntingtin (Htt) gene with Huntington's disease (HD) is unknown. Prior work suggests a role for Htt in neuronal autophagic function and mutant HTT protein disrupts autophagic cargo loading. Reductions in the...
Alan Diot et al.
Pharmacological research, 100, 24-35 (2015-07-22)
Mitophagy is a cellular mechanism for the recycling of mitochondrial fragments. This process is able to improve mitochondrial DNA (mtDNA) quality in heteroplasmic mtDNA disease, in which mutant mtDNA co-exists with normal mtDNA. In disorders where the load of mutant...
Hamza Ibrahim Isa et al.
Toxicon : official journal of the International Society on Toxinology, 164, 20-25 (2019-04-05)
Intoxication by Moraea pallida Bak. (yellow tulp) in livestock is of great importance in South Africa, ranking top among all plant-induced cardiac glycoside toxicosis. The toxic principle, a bufadienolide, is 1α, 2α-epoxyscillirosidine. Treatment of poisoning is challenging and affected livestock...
Stephanie M Kim et al.
Neuroendocrinology, 107(4), 387-399 (2018-10-24)
Elevated levels of saturated fatty acids (SFA) induce a state of neuroinflammation in the hypothalamus. It has been suggested that microglia sense palmitate, a prevalent circulating SFA, and act as mediators of this inflammatory process by communicating with neurons, particularly...
Aisling M Ross et al.
Materials science & engineering. C, Materials for biological applications, 113, 110985-110985 (2020-06-04)
Delivering therapeutics to disease sites is a challenge facing modern medicine. Nanoparticle delivery systems are of considerable interest to overcome this challenge, but these systems suffer from poor clinical translation. It is believed this is, in part, due to incomplete...
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Signe Christensen et al.
PloS one, 14(9), e0222595-e0222595 (2019-09-20)
Chlamydia trachomatis is an obligate intracellular bacterium with a distinctive biphasic developmental cycle that alternates between two distinct cell types; the extracellular infectious elementary body (EB) and the intracellular replicating reticulate body (RB). Members of the genus Chlamydia are dependent...
Jane Gilmour et al.
Epigenetics & chromatin, 12(1), 33-33 (2019-06-06)
Both tissue-specific and ubiquitously expressed transcription factors, such as Sp-family members, are required for correct development. However, the molecular details of how ubiquitous factors are involved in programming tissue-specific chromatin and thus participate in developmental processes are still unclear. We...
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Teneale A Stewart et al.
Laboratory investigation; a journal of technical methods and pathology, 100(2), 224-233 (2019-06-28)
The Ca2+ signal is essential in both hypoxia- and epidermal growth factor (EGF)-mediated epithelial to mesenchymal transition (EMT) in MDA-MB-468 breast cancer cells. This finding suggests that Ca2+-permeable ion channels participate in the induction of expression of some mesenchymal markers...
Andrew M Tidball et al.
Human molecular genetics, 24(7), 1929-1944 (2014-12-10)
The essential micronutrient manganese is enriched in brain, especially in the basal ganglia. We sought to identify neuronal signaling pathways responsive to neurologically relevant manganese levels, as previous data suggested that alterations in striatal manganese handling occur in Huntington's disease...
Aintzane García-Bea et al.
Journal of psychopharmacology (Oxford, England), 31(12), 1519-1526 (2017-06-29)
As well as being expressed as a full-length transcript, the group II metabotropic glutamate receptor 3 (GRM3, mGlu3) gene is expressed as an mRNA isoform which lacks exon 4 (GRM3Δ4) and which is predicted to encode a protein with a...
Eleonora Mercatelli et al.
Biochimica et biophysica acta, 1863(2), 198-204 (2015-11-22)
The redox properties of cellular environments are critical to many functional processes, and are strictly controlled in all living organisms. The glutathione-glutathione disulfide (GSH-GSSG) couple is the most abundant intracellular redox couple. A GSH redox potential can be calculated for...
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Bart Kramer et al.
International journal of molecular sciences, 20(18) (2019-09-25)
Pancreatic Ductal Adenocarcinoma (PDAC) is one of the most lethal cancers due to a high chemoresistance and poor vascularization, which results in an ineffective systemic therapy. PDAC is characterized by a high intratumoral pressure, which is not captured by current...