Search Within
Applications
Content Type
Collections
Applied Filters:
Keyword:'Deoxynucleotide Mix, 10 mM'
Showing 1-30 of 554 results for "

Deoxynucleotide Mix, 10 mM

" within Site Content
End Point PCR Protocol for Long and Accurate DNA Amplification
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.
Long and Accurate PCR Amplification of DNA (D8045)
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.
Amplification of Damaged DNA with Restorase DNA Polymerase (R1028)
Method for amplification of DNA from damaged DNA sources. Particularly useful for DNA extracted from old samples.
Standard PCR Protocol
Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.
Long and Accurate PCR Amplification of DNA with RedAccuTaq® (D4812)
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. Red dye allows direct loading of reaction on a gel. REDAccuTaq LA
Low Contaminant Amplification of DNA Using MTP™ Taq DNA Polymerase
Method for bacterial genome analysis and detection of pathogens. Minimize false positive PCRs through lab design and reagents tested for use in bacterial PCR applications.
Amplification of DNA Using Jumpstart™ Taq DNA Polymerase (D4184)
Hot Start Taq Polymerase protocol to reduce non-specific amplification, with MgCl2 Optimization
Amplification of DNA Using Jumpstart™ Taq DNA Polymerase (D9307)
Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
Amplification of DNA Using Jumpstart™ REDTaq® DNA Polymerase (D8187)
Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
PCR Master Mix
A PCR master mix is a batch of PCR or RT-PCR reagents that can be divided among many PCR reaction tubes. It usually includes DNA polymerase, dNTPs, MgCl2 and buffer. Make your own master mix or choose a commercial one.
PCR Reagents & Kits
Polymerase Chain Reaction (PCR) is a mainstay in molecular biology labs. PCR is used for amplifying DNA by several orders of magnitude and our reagents and resources are suitable for numerous research workflows including, gene expression analysis, genotyping, sequencing, and...
NGS Library Preparation
Library preparation is the first step of next generation sequencing. Before DNA or RNA samples can be sequenced, nucleic acids must be isolated, fragmented, end-repaired, and covalently linked to adapters using ligation or tagmentation methods.
Coronavirus COVID-19 (SARS-CoV-2) Detection, Characterization, Vaccine and Therapy Production
We've consolidated our COVID-19 detection, characterization, and vaccine production product offers to help researchers responding to the coronavirus pandemic.
Creating Transgenic Mice using CRISPR-Cas9 Genome Editing
Creating Transgenic Mice using CRISPR-Cas9 Genome Editing
Nucleic Acid Modifying Enzyme Selection Chart (Ultra Pure)
A helpful chart for selection of correct nucleic acid modifying enzyme.
Working with PCR
General Considerations; Factors to Consider in RT-PCR; Prevention of Carryover Contamination; Troubleshooting
Preactivated Magnetic Beads for Affinity Chromatography
Covalent coupling of antibodies, aptamers, and proteins with a NHS Mag Sepharose, Sera-Mag Carboxylate-Modified, Sera-Mag SpeedBeads Carboxylate-Modified from Cytiva.
dNTP Mediated Hot Start PCR Protocol
Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecting group is removed during a heat activation step.
Enzymatic Asparaginase Activity Assay
This assay protocol is suitable for the colorimetric/fluorometric detection of Asparaginase activity in bacteria, cell and tissue culture supernatants, plant samples, biological samples, and food samples using the Asparaginase Activity Assay Kit (MAK007).
Enzymatic Method for Determining Alanine (Alanine Assay)
Assay protocol for the colorimetric/fluorometric detection of Alanine in biological samples using the alanine assay kit.
MTP Activity Assay Kit Protocol
The MTP Activity Assay Kit is a sensitive, homogeneous fluorometric assay useful for continuous measurement of MTP activity in cell lysate or tissue homogenate.
Alkaline Phosphatase Protocol
Alkaline phosphatase (AP) is a non-specific phosphomonoester hydrolase that catalyzes the hydrolysis of a wide variety of organic monophosphates.
KAPA Mouse Genotyping Kit FAQs
Frequently asked questions (FAQs) for KAPA Mouse Genotyping Kit.
Enzymatic Method for Determining D-Sorbitol (D-Sorbitol Assay)
Assay protocol for the colorimetric detection of D-Sorbitol in samples using the D-Sorbitol assay kit.
Enzymatic Method for Determining Galactose (Galactose Assay)
This assay protocol is suitable for the colorimetric/fluorometric detection of Galactose and Lactose in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples using the Galactose Assay Kit.
Enzymatic Method for Determining Glucose-6-Phosphate (Glucose-6-Phosphate Assay)
Assay protocol for the colorimetric detection of glucose-6-phosphate in biological samples using the glucose-6-phosphate assay kit.
Cleavage and Purification of GST-Tagged Protein Bound to GSTrap
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
End-Point PCR: Antibody-Mediated Hot Start PCR Protocol with Enhanced Specificity and Yield
Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
Enzymatic Method for Determining Phenylalanine (Phenylalanine Assay)
Assay protocol for the fluorometric detection of Phenylalanine in biological samples using the phenylalanine assay kit.
Alkaline Phosphatase (AP) Protocol
Alkaline phosphatase (AP) is a non-specific phosphomonoester hydrolase that catalyzes the hydrolysis of a wide variety of organic monophosphates.
Page 1 of 19
Page 1 of 19