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Marie-France Portnoi et al.
Human molecular genetics, 27(7), 1228-1240 (2018-01-27)
SOX8 is an HMG-box transcription factor closely related to SRY and SOX9. Deletion of the gene encoding Sox8 in mice causes reproductive dysfunction but the role of SOX8 in humans is unknown. Here, we show that SOX8 is expressed in
Federico Bernal et al.
Cancer cell, 18(5), 411-422 (2010-11-16)
Cancer cells neutralize p53 by deletion, mutation, proteasomal degradation, or sequestration to achieve a pathologic survival advantage. Targeting the E3 ubiquitin ligase HDM2 can lead to a therapeutic surge in p53 levels. However, the efficacy of HDM2 inhibition can be
Theresa J Pell et al.
SLAS discovery : advancing life sciences R & D, 26(7), 909-921 (2021-06-05)
A core aspect of epithelial cell function is barrier integrity. A loss of barrier integrity is a feature of a number of respiratory diseases, including asthma, allergic rhinitis, and chronic obstructive pulmonary disease. Restoration of barrier integrity is a target
Roberto Mendez et al.
Bio-protocol, 7(21) (2017-11-25)
In this protocol, we describe proximal ligation assay (PLA), an antibody-based detection method for protein-protein interaction. This method relies on specific binding of individual primary antibodies to the two putative interacting proteins. The primary antibodies need to have different hosts.
Geng-Xian Shi et al.
Proceedings of the National Academy of Sciences of the United States of America, 115(2), E190-E199 (2017-12-28)
Directed migration is essential for cell motility in many processes, including development and cancer cell invasion. RSKs (p90 ribosomal S6 kinases) have emerged as central regulators of cell migration; however, the mechanisms mediating RSK-dependent motility remain incompletely understood. We have
Yoshitsugu Aoki et al.
Brain : a journal of neurology, 140(4), 887-897 (2017-03-24)
A non-coding hexanucleotide repeat expansion in intron 1 of the C9orf72 gene is the most common cause of amyotrophic lateral sclerosis and frontotemporal dementia (C9ALS/FTD), however, the precise molecular mechanism by which the C9orf72 hexanucleotide repeat expansion directs C9ALS/FTD pathogenesis
Trevor M Morey et al.
Frontiers in molecular neuroscience, 10, 415-415 (2018-01-10)
Choline acetyltransferase (ChAT) synthesizes the neurotransmitter acetylcholine in cholinergic neurons, and mutations of this enzyme are linked to the neuromuscular disorder congenital myasthenic syndrome (CMS). One CMS-related mutation, V18M, reduces ChAT enzyme activity and cellular protein levels, and is located
Ingrid Nilsson et al.
The EMBO journal, 29(8), 1377-1388 (2010-03-13)
The vascular endothelial growth factors VEGFA and VEGFC are crucial regulators of vascular development. They exert their effects by dimerization and activation of the cognate receptors VEGFR2 and VEGFR3. Here, we have used in situ proximity ligation to detect receptor
S Germann et al.
Oncogene, 31(42), 4536-4549 (2012-01-24)
Ddx5 and ddx17 are two highly related RNA helicases involved in both transcription and splicing. These proteins coactivate transcription factors involved in cancer such as the estrogen receptor alpha, p53 and beta-catenin. Ddx5 and ddx17 are part of the splicing
Leonid A Serebryannyy et al.
Methods (San Diego, Calif.), 157, 80-87 (2018-11-13)
Protein-protein interactions are essential for cellular structure and function. To delineate how the intricate assembly of protein interactions contribute to cellular processes in health and disease, new methodologies that are both highly sensitive and can be applied at large scale
Melanie Spears et al.
Breast cancer research and treatment, 132(2), 463-470 (2011-06-04)
HER2 overexpression/amplification is linked with poor prognosis in early breast cancer. Co-expression of HER2 and HER3 is associated with endocrine and chemotherapy resistance, driven not simply by expression but by signalling via HER2:HER3 or HER2:HER2 dimers. Proximity ligation assays (PLAs)
Meritxell Balmaña et al.
International journal of biological macromolecules, 112, 33-45 (2018-02-07)
Pancreatic adenocarcinoma (PDAC) lacks efficient biomarkers. Mucins are glycoproteins that can carry aberrant glycosylation in cancer. Our objective was to identify cancer-related glycan epitopes on MUC1 and MUC5AC mucins in PDAC as potential biomarkers. We have analysed the tumour-associated carbohydrate
Bernardo Blanco-Sánchez et al.
Cell reports, 25(5), 1281-1291 (2018-11-01)
Morphogenesis and mechanoelectrical transduction of the hair cell mechanoreceptor depend on the correct assembly of Usher syndrome (USH) proteins into highly organized macromolecular complexes. Defects in these proteins lead to deafness and vestibular areflexia in USH patients. Mutations in a non-USH
Pengcheng Zhu et al.
Cancer cell, 19(3), 401-415 (2011-03-15)
Cancer is a leading cause of death worldwide. Tumor cells exploit various signaling pathways to promote their growth and metastasis. To our knowledge, the role of angiopoietin-like 4 protein (ANGPTL4) in cancer remains undefined. Here, we found that elevated ANGPTL4
Joris Wauman et al.
Biochimica et biophysica acta. Molecular cell research, 1865(9), 1312-1325 (2018-06-23)
Hypothalamic leptin receptor (LR) signaling regulates body weight by balancing food intake and energy expenditure. It is well established that the human LR undergoes ectodomain shedding, but little is known about the fate of the remaining cytosolic domain. This study
Tamás Sticz et al.
Pathology oncology research : POR (2018-06-09)
Several monoclonal antibodies and inhibitors targeting signalling pathways are being used in personalised medicine. Anti-EGFR antibodies seem to be effective, however, therapy resistance often occurs in colon carcinoma cases. mTOR inhibitors (mTORIs) could have a potential role in the breakthrough
Nadine Kirsch et al.
Developmental cell, 43(1), 71-82 (2017-10-11)
Angiopoietin-like 4 (ANGPTL4) is a secreted signaling protein that is implicated in cardiovascular disease, metabolic disorder, and cancer. Outside of its role in lipid metabolism, ANGPTL4 signaling remains poorly understood. Here, we identify ANGPTL4 as a Wnt signaling antagonist that
Jelena Katic et al.
Journal of cell science, 130(15), 2606-2619 (2017-06-21)
The immunoglobulin superfamily adhesion molecule close homolog of L1 (CHL1) plays important roles during nervous system development. Here, we identified the hedgehog receptor patched-1 (PTCH1) as a novel CHL1-binding protein and showed that CHL1 interacts with the first extracellular loop
Ming-Xin An et al.
The Journal of cell biology, 216(12), 4091-4105 (2017-11-09)
Aerobic glycolysis, a phenomenon known historically as the Warburg effect, is one of the hallmarks of cancer cells. In this study, we characterized the role of BAG3 in aerobic glycolysis of pancreatic ductal adenocarcinoma (PDAC) and its molecular mechanisms. Our
Lan Dan et al.
Haematologica, 97(4), 551-559 (2011-12-31)
Inhibitors of nicotinamide phosphoribosyltransferase have recently been validated as therapeutic targets in leukemia, but the mechanism of leukemogenic transformation downstream of this enzyme is unclear. Here, we evaluated whether nicotinamide phosphoribosyltransferase's effects on aberrant proliferation and survival of myeloid leukemic
Stefano Sechi et al.
Open biology, 7(1) (2017-01-20)
Cytokinesis requires a tight coordination between actomyosin ring constriction and new membrane addition along the ingressing cleavage furrow. However, the molecular mechanisms underlying vesicle trafficking to the equatorial site and how this process is coupled with the dynamics of the
Ajay K Yadav et al.
JAMA, 302(3), 276-289 (2009-07-16)
Glioblastomas--uniformly fatal brain tumors--often have both monosomy of chromosome 10 and gains of the epidermal growth factor receptor (EGFR) gene locus on chromosome 7, an association for which the mechanism is poorly understood. To assess whether coselection of EGFR gains
Bettina P Mihalas et al.
The Journal of biological chemistry, 293(49), 18944-18964 (2018-10-12)
An increase in oxidative protein damage is a leading contributor to the age-associated decline in oocyte quality. By removing such damaged proteins, the proteasome plays an essential role in maintaining the fidelity of oocyte meiosis. In this study, we established
Volkan Turp et al.
Journal of esthetic and restorative dentistry : official publication of the American Academy of Esthetic Dentistry ... [et al.], 30(4), 360-368 (2018-08-03)
To evaluate the influence of anterior monolithic zirconia and lithium disilicate thickness on polymerization efficiency of dual-cure resin cements. Twelve ceramic disks (4.0-mm diameter) with thicknesses of 0.5, 1, 1.5, 2, 2.5, and 3 mm were prepared from monolithic zirconia
Alexander Munden et al.
eLife, 7 (2018-10-03)
Control of DNA copy number is essential to maintain genome stability and ensure proper cell and tissue function. In Drosophila polyploid cells, the SNF2-domain-containing SUUR protein inhibits replication fork progression within specific regions of the genome to promote DNA underreplication.
Yong Hu et al.
Journal of virology, 91(8) (2017-02-06)
Severe acute respiratory syndrome (SARS) is a respiratory disease, caused by a coronavirus (SARS-CoV), that is characterized by atypical pneumonia. The nucleocapsid protein (N protein) of SARS-CoV plays an important role in inhibition of type I interferon (IFN) production via
Ali Asghar Alavi et al.
Journal of dentistry (Shiraz, Iran), 18(1), 50-55 (2017-03-11)
Porcelain laminate veneer is an esthetic restoration used as an alternative to full veneer crowns and requires minimal tooth preparation. In restoration with porcelain laminate veneers, both the longevity of the laminate and conservation of the sound tooth structure are
Yuxuan Miao et al.
Cell host & microbe, 22(3), 330-342 (2017-09-15)
Rab small GTPases control membrane trafficking through effectors that recruit downstream mediators such as motor proteins. Subcellular trafficking typically involves multiple Rabs, with each specific step mediated by a distinct Rab protein. We describe a collaboration between two distinct Rab-protein-orchestrated
Joanna Korfanty et al.
Reproduction (Cambridge, England), 156(3), 195-206 (2018-06-09)
SPEN (spen family transcription repressor) is a nucleic acid-binding protein putatively involved in repression of gene expression. We hypothesized that SPEN could be involved in general downregulation of the transcription during the heat shock response in mouse spermatogenic cells through
Leire Almandoz-Gil et al.
Frontiers in neurology, 9, 180-180 (2018-04-07)
The aggregation of alpha-synuclein (αSyn) is the pathological hallmark of Parkinson's disease, dementia with Lewy bodies and related neurological disorders. However, the physiological function of the protein and how this function relates to its pathological effects remain poorly understood. One
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