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Atefeh Mousavi Nik et al.
Molecular pharmacology, 92(1), 88-99 (2017-04-22)
Fluorometric imaging plate reader membrane potential dye (FMP-Red-Dye) is a proprietary tool for basic discovery and high-throughput drug screening for G-protein-coupled receptors and ion channels. We optimized and validated this potentiometric probe to assay functional modulators of heterologous expressed GABA
Alana L Rister et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1137, 121941-121941 (2019-12-27)
Methods for the analysis of steroids have long been of interest due to the multiple uses for such methods in medical applications, sports monitoring, and environmental science. The analysis of steroids involves inherent analytical hurdles due to their low biological
Christopher C Waller et al.
Steroids, 92, 74-80 (2014-10-07)
Steroid sulfates are a major class of steroid metabolite that are of growing importance in fields such as anti-doping analysis, the detection of residues in agricultural produce or medicine. Despite this, many steroid sulfate reference materials may have limited or
Christopher C Waller et al.
Drug testing and analysis, 12(6), 752-762 (2020-01-18)
Hemapolin (2α,3α-epithio-17α-methyl-5α-androstan-17β-ol) is a designer steroid that is an ingredient in several "dietary" and "nutritional" supplements available online. As an unusual chemical modification to the steroid A-ring could allow this compound to pass through antidoping screens undetected, the metabolism of
Rakhee Gupte et al.
American journal of physiology. Heart and circulatory physiology, 319(1), H144-H158 (2020-05-23)
Pyridine nucleotides, such as NADPH and NADH, are emerging as critical players in the regulation of heart and vascular function. Glucose-6-phosphate dehydrogenase (G6PD), the rate-limiting enzyme in the pentose phosphate pathway, is the primary source and regulator of cellular NADPH.
Christopher D Chouinard et al.
Journal of the American Society for Mass Spectrometry, 28(2), 323-331 (2016-12-04)
Ion mobility-mass spectrometry (IM-MS) has recently seen increased use in the analysis of small molecules, especially in the field of metabolomics, for increased breadth of information and improved separation of isomers. In this study, steroid epimers androsterone and trans-androsterone were
Julianne Cook Botelho et al.
Analytical chemistry, 88(22), 11123-11129 (2016-10-18)
The inaccuracy of 17-β estradiol (E2) measurements affects its use as a biomarker in patient care and research. Clinical and research communities called for accurate and standardized E2 measurements. Reference Measurement Procedures (RMPs), part of the CDC Hormone Standardization Program
Argitxu Esquivel et al.
Drug testing and analysis, 11(3), 392-402 (2018-10-27)
The detection of testosterone (T) misuse is performed using the steroid profile that includes concentrations of T and related metabolites excreted free and glucuronoconjugated, and the ratios between them. In this work, the usefulness of 14 endogenous steroid sulfates to
Monica M Laronda et al.
Nature communications, 8, 15261-15261 (2017-05-17)
Emerging additive manufacturing techniques enable investigation of the effects of pore geometry on cell behavior and function. Here, we 3D print microporous hydrogel scaffolds to test how varying pore geometry, accomplished by manipulating the advancing angle between printed layers, affects
Hiroyuki Sato et al.
Journal of medicinal chemistry, 51(6), 1831-1841 (2008-03-01)
TGR5, a metabotropic receptor that is G-protein-coupled to the induction of adenylate cyclase, has been recognized as the molecular link connecting bile acids to the control of energy and glucose homeostasis. With the aim of disclosing novel selective modulators of
Laurie De Wilde et al.
Drug testing and analysis, 12(8), 1006-1018 (2020-04-09)
In doping control, to confirm the exogenous origin of exogenously administered anabolic androgenic steroids (AAS), a gas chromatography combustion isotope ratio mass spectrometry (GC-C-IRMS) analysis is performed. Recently published work suggests that epiandrosterone sulfate (EpiAS) is a promising IRMS target
Argitxu Esquivel et al.
Drug testing and analysis, 11(8), 1218-1230 (2019-04-02)
The introduction of alternative markers to the steroid profile can be an effective approach to improving the screening capabilities for the detection of testosterone (T) misuse. In this work, endogenous steroid sulfates were evaluated as potential markers to detect intramuscular
Monika Šrámková et al.
Steroids, 121, 47-53 (2016-11-23)
Anxiety and mood disorders (AMD) are the most frequent mental disorders in the human population. They have recently shown increasing prevalence, and commonly disrupt personal and working lives. The aim of our study was to analyze the spectrum of circulating
Seun F Akomolafe et al.
The Journal of pharmacy and pharmacology, 71(10), 1565-1575 (2019-08-07)
This study was designed to assess the role of caffeine on fertility parameters in testicular and epididymal tissues of scopolamine-induced model of amnesia in rats. Adult male rats were treated with scopolamine with or without caffeine. The modulatory effects of
Anna Panek et al.
Molecules (Basel, Switzerland), 25(18) (2020-09-19)
The biotransformation of steroid compounds is a promising, environmentally friendly route to new pharmaceuticals and hormones. One of the reaction types common in the metabolic fate of steroids is Baeyer-Villiger oxidation, which in the case of cyclic ketones, such as
K C Nicolaou et al.
The Journal of organic chemistry, 86(3), 2499-2521 (2021-01-09)
Thailanstatin A and spliceostatin D, two naturally occurring molecules endowed with potent antitumor activities by virtue of their ability to bind and inhibit the function of the spliceosome, and their natural siblings and designed analogues, constitute an appealing family of
Adriana M Cirigliano et al.
Phytochemical analysis : PCA, 31(5), 606-615 (2020-02-12)
An efficient characterisation of metabolites is a crucial task in many aspects of basic research, such as the de-replication of crude extracts in natural products chemistry or the tentative identification of compounds in untargeted metabolomics. The goal of this work
Artur T Cordeiro et al.
Bioorganic & medicinal chemistry, 18(13), 4762-4768 (2010-06-24)
Glucose 6-phosphate dehydrogenase (G6PDH) catalyzes the first step of the pentose-phosphate pathway which supplies cells with ribose 5-phosphate (R5P) and NADPH. R5P is the precursor for the biosynthesis of nucleotides while NADPH is the cofactor of several dehydrogenases acting in
Edith Bellavance et al.
Journal of medicinal chemistry, 52(23), 7488-7502 (2009-09-24)
17beta-Hydroxysteroid dehydrogenase type 7 (17beta-HSD7) catalyzes the reduction of estrone (E(1)) into estradiol (E(2)) and of dihydrotestosterone (DHT) into 5alpha-androstane-3beta,17beta-diol (3beta-diol), therefore modulating the level of mitogenic estrogens and androgens in humans. By classical and parallel chemistry, we generated several
Julia V Bukanova et al.
Frontiers in molecular neuroscience, 13, 44-44 (2020-04-09)
The ability of androstane and androstene neurosteroids with modifications at C-17, C-5, and C-3 (compounds 1-9) to influence the functional activity of inhibitory glycine and γ-aminobutyric acid (GABA) receptors was estimated. The glycine- and GABA-induced chloride current (IGly and IGABA)
Artur T Cordeiro et al.
Bioorganic & medicinal chemistry, 17(6), 2483-2489 (2009-02-24)
Dehydroepiandrosterone (DHEA) is known as an intermediate in the synthesis of mammalian steroids and a potent uncompetitive inhibitor of mammalian glucose-6-phosphate dehydrogenase (G6PDH), but not the enzyme from plants and lower eukaryotes. G6PDH catalyzes the first step of the pentose-phosphate
D Hofer et al.
The Journal of clinical endocrinology and metabolism, 99(10), 3766-3773 (2014-06-18)
The vitamin D system has pleiotropic effects not only in bone metabolism. Its role in testicular steroidogenesis is new and deserves intensive research. We hypothesize that vitamin D, especially 1,25 dihydroxyvitamin D3 [1,25(OH)2D3 (calcitriol)] induces male steroidogenesis and intend to
Dayamin Martinez-Brito et al.
Drug testing and analysis, 11(11-12), 1629-1643 (2019-11-09)
7-keto-DHEA (3β-hydroxy-androst-5-ene-7,17-dione) is included in section S1 of the World Antidoping Agency (WADA) List of Prohibited Substances. The detection of its misuse in sports needs special attention, since it is naturally present in urine samples. The main goal of this
Validation of steroid sulfates deconjugation for metabolic studies. Application to human urine samples.
Martinez-Brito, et al.
Journal of Pharmacological and Toxicological Methods, 106, 106938-106938 (2020)
Sung-Hee Cho et al.
Neurochemical research, 45(9), 2044-2051 (2020-07-01)
Valproic acid (VPA), an antiepileptic and mood stabilizer, modulates neurotransmission and gene expression by inhibiting histone deacetylase activity. It is reported that VPA may affects the steroid hormone level. In this study, VPA-induced acute metabolic alterations were investigated using liquid
Xiaoguang Liu et al.
Nature cell biology, 22(4), 476-486 (2020-04-02)
SLC7A11-mediated cystine uptake is critical for maintaining redox balance and cell survival. Here we show that this comes at a significant cost for cancer cells with high levels of SLC7A11. Actively importing cystine is potentially toxic due to its low
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