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E5134

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Elizabeth Spiegler et al.
Scientific reports, 8(1), 8834-8834 (2018-06-13)
Vitamin A deficiency is still a public health concern affecting millions of pregnant women and children. Retinoic acid, the active form of vitamin A, is critical for proper mammalian embryonic development. Embryos can generate retinoic acid from maternal circulating β-carotene...
Peter Schuff-Werner et al.
British journal of haematology, 162(5), 684-692 (2013-07-03)
Pseudothrombocytopenia remains a challenge in the haematological laboratory. The pre-analytical problem that platelets tend to easily aggregate in vitro, giving rise to lower platelet counts, has been known since ethylenediamine-tetra acetic acid EDTA and automated platelet counting procedures were introduced...
Joshua E Lewis et al.
Cell systems, 12(1), 68-81 (2021-01-22)
Redox cofactor production is integral toward antioxidant generation, clearance of reactive oxygen species, and overall tumor response to ionizing radiation treatment. To identify systems-level alterations in redox metabolism that confer resistance to radiation therapy, we developed a bioinformatics pipeline for...
Kazi Mirajul Hoque et al.
Nature communications, 11(1), 2767-2767 (2020-06-04)
The pathophysiological nature of the common ABCG2 gout and hyperuricemia associated variant Q141K (rs2231142) remains undefined. Here, we use a human interventional cohort study (ACTRN12615001302549) to understand the physiological role of ABCG2 and find that participants with the Q141K ABCG2...
Yujin E Kim et al.
Nature protocols, 5(8), 1396-1405 (2010-07-31)
Applications for noninvasive real-time imaging of apoptosis and neuronal degeneration are hindered by technical limitations in imaging strategies and by existing probes. Monitoring the progression of a cell through apoptosis could provide valuable insight into the temporal events that initiate...
Jessica Elswood et al.
Autophagy, 17(2), 420-438 (2020-01-28)
Mitochondria operate as a central hub for many metabolic processes by sensing and responding to the cellular environment. Developmental cues from the environment have been implicated in selective autophagy, or mitophagy, of mitochondria during cell differentiation and tissue development. Mitophagy...
Vassiliki Stamatopoulou et al.
Nature protocols, 13(10), 2387-2406 (2018-09-27)
Ribosome biogenesis is initiated in the nucleolus, a cell condensate essential to gene expression, whose morphology informs cancer pathologists on the health status of a cell. Here, we describe a protocol for assessing, both qualitatively and quantitatively, the involvement of...
Olivier Loudig et al.
International journal of molecular sciences, 18(3) (2017-03-25)
Formalin-fixed paraffin-embedded (FFPE) specimens, when used in conjunction with patient clinical data history, represent an invaluable resource for molecular studies of cancer. Even though nucleic acids extracted from archived FFPE tissues are degraded, their molecular analysis has become possible. In...
Gunnar F Kwakye et al.
Current protocols in toxicology, Chapter 12, Unit12-Unit12 (2011-05-10)
Cellular manganese (Mn) uptake and transport dynamics can be measured using a cellular fura-2 manganese extraction assay (CFMEA). The assay described here uses immortalized murine striatal cell line and primary cortical astrocytes, but the method is equally adaptable to other...
Xiang Gao et al.
Acta radiologica (Stockholm, Sweden : 1987), 55(3), 328-334 (2013-08-22)
Previous studies have demonstrated that magnetic resonance imaging may be a method of choice to visualize transplanted pancreatic islets. However, contrast agents may interfere with microcirculation and affect graft function. To evaluate the effects manganese-containing contrast media on regional blood...
Elin Hadler-Olsen et al.
Journal of cellular physiology, 230(1), 160-169 (2014-06-07)
Matrix metalloproteinase 2 (MMP-2) is a proteolytic enzyme implicated in motility, differentiation, and regeneration of skeletal muscle fibers through processing of extracellular substrates. Although MMP-2 has been found to be localized intracellularly in cardiomyocytes where the enzyme is thought to...
Marie-Laurence Lemay et al.
Bio-protocol, 8(1), e2674-e2674 (2018-01-05)
This protocol describes a straightforward method to generate specific mutations in the genome of strictly lytic phages. Briefly, a targeting CRISPR-Cas9 system and a repair template suited for homologous recombination are provided inside a bacterial host, here the Gram-positive model...
Marlies De Boeck et al.
Mutation research. Genetic toxicology and environmental mutagenesis, 786-788, 151-157 (2015-07-28)
As part of the Japanese Center for the Validation of Alternative Methods (JaCVAM)-initiated international validation study of in vivo rat alkaline comet assay (comet assay), p-phenylenediamine dihydrochloride (PPD), o-phenylphenol sodium salt (OPP), and 2,4-diaminotoluene (2,4-DAT), were analyzed in this laboratory...
Shiaoching Gong et al.
Nature protocols, 5(10), 1678-1696 (2010-10-05)
We report here a high-throughput method for the modification of bacterial artificial chromosomes (BACs) that uses a novel two-plasmid approach. In this protocol, a vector modified in our laboratory to hold an R6Kγ origin of replication and a marker recombination...
Lynnae D Hyatt et al.
PloS one, 9(10), e109072-e109072 (2014-10-10)
Zinc finger protein 36, C3H type-like 1 (ZFP36L1) is one of several Zinc Finger Protein 36 (Zfp36) family members, which bind AU rich elements within 3' untranslated regions (UTRs) to negatively regulate the post-transcriptional expression of targeted mRNAs. The prototypical...
Sweety Samal et al.
MethodsX, 6, 837-849 (2019-05-03)
An ideal vaccine against HIV-1 will specifically elicit bNAbs (broadly neutralizing antibodies) which can cross-neutralize a wide spectrum of circulating viral strains belonging to different clades. The current paradigm for developing such a vaccine is to generate HIV-1 envelope (Env)-based...
Xiang Wu et al.
BMC microbiology, 11, 87-87 (2011-04-30)
The periplasmic High Temperature Requirement protein A (HtrA) plays important roles in bacterial protein folding and stress responses. However, the role of chlamydial HtrA (cHtrA) in chlamydial pathogenesis is not clear. The cHtrA was detected both inside and outside the...
Fan Xia et al.
Molecular biotechnology, 61(5), 325-331 (2019-02-24)
The intestinal epithelium is an important receptor that is not only exposed to nutrients but also to pathogens, such as ingested toxins, bacterial flora, and their metabolites. The sensory information is communicated to extensive endocrine, neural, immune systems, and the...
Zhoumeng Lin et al.
Toxicological sciences : an official journal of the Society of Toxicology, 141(1), 90-102 (2014-06-11)
Atrazine (ATR) is one of the most frequently detected pesticides in the U.S. water supply. This study aimed to investigate neurobehavioral and neurochemical effects of ATR in C57BL/6 mouse offspring and dams exposed to a relatively low (3 mg/l, estimated...
Jihwan Lee et al.
Science advances, 6(43) (2020-10-25)
Unraveling the genetic and epigenetic determinants of phenotypes is critical for understanding and re-engineering biology and would benefit from improved methods to separate cells based on phenotypes. Here, we report SPOTlight, a versatile high-throughput technique to isolate individual yeast or...
Elzbieta Janda et al.
Antioxidants (Basel, Switzerland), 10(5) (2021-06-03)
Dietary flavonoids stimulate autophagy and prevent liver dysfunction, but the upstream signaling pathways triggered by these compounds are not well understood. Certain polyphenols bind directly to NRH-quinone oxidoreductase 2 (NQO2) and inhibit its activity. NQO2 is highly expressed in the...
Ivana Savic Azoulay et al.
Journal of neurochemistry, 153(2), 203-215 (2020-01-25)
Acid-sensing ion channel 1a (ASIC1a) is well-known to play a major pathophysiological role during brain ischemia linked to acute acidosis of ~pH 6, whereas its function during physiological brain activity, linked to much milder pH changes, is still poorly understood....
Sylvie Delcambre et al.
Frontiers in neurology, 11, 881-881 (2020-09-29)
Several mutations in leucine-rich repeat kinase-2 (LRRK2) have been associated with Parkinson's disease (PD). The most common substitution, G2019S, interferes with LRRK2 kinase activity, which is regulated by autophosphorylation. Yet, the penetrance of this gain-of-function mutation is incomplete, and thus...
Kathryn A Solka et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 64(11), 647-668 (2016-09-30)
Lubricin is a secreted, mucin-like glycoprotein and proteoglycan abundant in synovial fluid that provides boundary lubrication and prevents cell adhesion in synovial joints. The antilubricin S6.79 monoclonal antibody recognizes an O-linked glycopeptide epitope in lubricin's mucin domain. The central, long...
Aditya Pandey et al.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 92(4), 269-278 (2014-06-20)
G-protein coupled receptors (GPCRs) are inherently dynamic membrane protein modulators of various important cellular signaling cascades. The apelin receptor (AR or APJ) is a class A GPCR involved in numerous physiological processes, implicated in angiogenesis during tumour formation and as...
Christian J F Bertens et al.
International journal of pharmaceutics, 570, 118641-118641 (2019-08-26)
Current information about the pharmacokinetics of an ocular drug can only be achieved by invasive sampling. However, confocal Raman spectroscopy bears the potential to quantify drug concentrations non-invasively. In this project, we evaluated the detection and quantification of ocular ketorolac...
Santiago González-López et al.
The journal of adhesive dentistry, 15(5), 481-489 (2013-04-18)
To compare the percentage of collagen exposed in dentin root thirds after two irrigation protocols with manual or rotary instrumentation using two methacrylate resin-based sealers. Forty-eight single-root human teeth were prepared with manual (n = 24) or nickeltitanium ProFile rotary...
Mary K Sherwood et al.
Chemosphere, 113, 56-61 (2014-07-30)
Modified Fenton (MF) chemistry was tested in the laboratory to treat three diesel fuel-contaminated soils from the Canadian arctic rich in soil organic matter (SOM) and Fe oxides. Reactors were dosed with hydrogen peroxide (HP), and treatment was compared in...
Bassam Tawfik et al.
eLife, 10 (2021-03-23)
Synaptotagmins confer calcium-dependence to the exocytosis of secretory vesicles, but how coexpressed synaptotagmins interact remains unclear. We find that synaptotagmin-1 and synaptotagmin-7 when present alone act as standalone fast and slow Ca2+-sensors for vesicle fusion in mouse chromaffin cells. When...
Demetra S M Chatzileontiadou et al.
STAR protocols, 2(3), 100635-100635 (2021-06-15)
Understanding T-cell responses requires identifying viral peptides presented by human leukocyte antigens (HLAs). X-ray crystallography can be used to visualize their presentation. This protocol describes the expression, purification, and crystallization of HLA-A∗02:01, one of the most frequent HLA in the...
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