again. To prevent polymerization, store ECM Gel on ice during handling.
5. Do I need to filter the Stem Cell Qualified ECM Gel before use?
No, ECM Gel is cell culture tested and
microbial growth. The ECM gel is further
purified to reduce the level of growth factors.
The ECM Gel, Growth Factor Reduced was developed
for applications requiring a further purified ECM. It
provides a
20 °C for long-term storage. ECM gel may
be stored at 2-8 °C for up to 72 hrs.
E1270dat Rev 05/21
Procedure
Thaw gel overnight at 2-8 C before use. Dispense gel
to wells
of any exogenous growth
factors. Our tissue-specific dECM Gel Hydrogel Kits are a series of native decellularized extracellular matrix (ECM) protein
solutions derived from a variety of non-diseased porcine
of any exogenous growth
factors. Our tissue-specific dECM Gel Hydrogel Kits are a series of native decellularized extracellular matrix (ECM) protein
solutions derived from a variety of non-diseased porcine
of any exogenous growth
factors. Our tissue-specific dECM Gel Hydrogel Kits are a series of native decellularized extracellular matrix (ECM) protein
solutions derived from a variety of non-diseased porcine
of any exogenous growth
factors. Our tissue-specific dECM Gel Hydrogel Kits are a series of native decellularized extracellular matrix (ECM) protein
solutions derived from a variety of non-diseased porcine
of any exogenous growth
factors. Our tissue-specific dECM Gel Hydrogel Kits are a series of native decellularized extracellular matrix (ECM) protein
solutions derived from a variety of non-diseased porcine
of any exogenous growth
factors. Our tissue-specific dECM Gel Hydrogel Kits are a series of native decellularized extracellular matrix (ECM) protein
solutions derived from a variety of non-diseased porcine
2
• Coating culture vessels with ECM Gel –
ECM Gel (Catalog Number E1270) is
recommended for use with Stemline Complete
Medium.
Note: The ECM Gel will gel within 5 minutes at
20 °C. For prolonged
of any exogenous growth
factors. Our tissue-specific dECM Gel Hydrogel Kits are a series of native decellularized extracellular matrix (ECM) protein
solutions derived from a variety of non-diseased porcine
of
cells to the extracellular matrix (ECM). Given Type I
collagen is an abundant component of the ECM, cells
cultured in three dimensional (3D) collagen gels
simulate the in vivo cell physiology better
of
cells to the extracellular matrix (ECM). Given Type I
collagen is an abundant component of the ECM, cells
cultured in three dimensional (3D) collagen gels
simulate the in vivo cell physiology better
20 °C.
Coating culture vessels with ECM Gel –
ECM Gel (Catalog Number E1270) is
recommended for use with Stemline Complete
Medium.
Note: The ECM Gel will gel within 5 minutes at
20 °C. For prolonged
the ECM while other disease states may be caused
by changes in the density or rigidity of the ECM. Since
Type I collagen is a key determinant of tensile
properties of the ECM, 3D collagen gels are useful
20 °C.
Coating culture vessels with ECM Gel –
ECM Gel (Catalog Number E1270) is
recommended for use with Stemline Complete
Medium.
Note: The ECM Gel will gel within 5 minutes at
20 °C. For prolonged
20 °C.
Coating culture vessels with ECM Gel –
ECM Gel (Catalog Number E1270) is
recommended for use with Stemline Complete
Medium.
Note: The ECM Gel will gel within 5 minutes at
20 °C. For prolonged
wells of a ECM Gel coated 6-well plate. Below are general guidelines for the
coating of 6-well plates and culture flasks with Stem Cell Qualified ECM Gel.
1. Thaw Stem Cell Qualified ECM Gel on ice.
pre-culture period before bioreactor operation. The
presence of alginate gel within fibrous PGA scaffolds reduced the
loss of ECM components from the constructs and obviated the
need for flow rate modulation
coated with 1:20 dilution of Stem Cell Qualified ECM Gel (Cat. No.
CC131-5ML). For detailed coating instructions, please refer to the Stem Cell Qualified ECM Gel (Cat. No. CC131-5ML) datasheet which is
available
at the bottom, which is coated with a gelled layer of ECM, usually collagen or
basement membrane extract. Cells are applied to the top of the gel, and a chemoattractant is
provided in the well below
to
effectively bind the ECM. Integrins bind many different
ligands including laminin. Each integrin is made up of a
large N-terminal extracellular domain that binds the
ECM ligand and a small C-terminal
to effectively bind the ECM.
Integrins bind many different ligands including laminin.
Each integrin is made up of a large N-terminal
extracellular domain that binds the ECM ligand and a
small C-terminal
to effectively bind the ECM.
Integrins bind many different ligands including laminin.
Each integrin is made up of a large N-terminal
extracellular domain that binds the ECM ligand and a
small C-terminal
measured and certified. The surfaces of the gels are
functionalized to form covalent bonds with amines on proteins. The
gel readily binds to purified ECM proteins, such as PureCol® type I
collagen
to effectively bind the ECM.
Integrins bind many different ligands including laminin.
Each integrin is made up of a large N-terminal
extracellular domain that binds the ECM ligand and a
small C-terminal
measured and certified. The surfaces of the gels are
functionalized to form covalent bonds with amines on proteins. The
gel readily binds to purified ECM proteins, such as PureCol® type I
collagen
to effectively bind the ECM.
Integrins bind many different ligands including laminin.
Each integrin is made up of a large N-terminal
extracellular domain that binds the ECM ligand and a
small C-terminal
measured and certified. The surfaces of the gels are
functionalized to form covalent bonds with amines on proteins. The
gel readily binds to purified ECM proteins, such as PureCol® type I
collagen