Protein gel electrophoresis is used to separate proteins for purification, characterization, and detection. Methods such as native PAGE, SDS-PAGE, 2D PAGE, and isoelectric focusing (IEF) are used in preparation for downstream applications including Western blotting, mass spectrometry, and proteomic analysis.
Genomic analysis tools for DNA and RNA detection and quantitation, including nucleic acid stains, sample loading buffers, precast agarose gels, transfer membranes, as well as biotin, fluorescein, and DIG RNA and DNA labeling reagents.
Learn the basics of DNA and RNA gel electrophoresis, and how these techniques can be used to separate and purify DNA and RNA molecules based on size and charge for cloning, PCR, mass spectrometry, next generation sequencing (NGS), and Northern...
Browse our products for protein electrophoresis and Western blotting, including Immobilon transfer membranes, transfer buffers, electrophoresis equipment, gels, gel stains, protein markers, and immunodetection reagents.
We offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments and RNA and can be concurrently run with the samples. All the markers...
To meet the great diversity of protein analysis needs, Sigma offers a wide selection of protein visualization (staining) reagents. EZBlue™ and ProteoSilver™, designed specifically for proteomics, also perform impressively in traditional PAGE formats.
The possible causes and potential remedies for challenges encountered during preparation of samples for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and optimizing electrophoresis conditions.
The Auto2D® 2-D Electrophoresis Device fully automates difficult 2D electrophoresis methods in a quick, easy, and reproducible way. Locate difficult-to-find proteins using the Auto2D® system in less than two hours with high reproducibility.
mPAGE precast polyacrylamide gels for electrophoresis provide high-resolution protein separation, along with a reduced cost and time-savings for users. mPAGE gels are compatible with common electrophoresis tanks and boast an increased sample well volume
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