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Showing 1-14 of 14 results for "EMS0004SIGMA" within Site Content
Evaluation of Recombinant, Chemically Treated Trypsin in Proteomics and Protein Characterization Assays
Evaluation of Recombinant, Chemically Treated Trypsin in Proteomics and Protein Characterization Assays
An Optimized Protocol for Peptide Mapping of Therapeutic Monoclonal Antibodies with Minimum Deamidation and Oxidation Artifacts
An optimized peptide mapping protocol using NISTmAb as a model monoclonal antibody, shorter incubation times, and improved digestion buffer to demonstrate minimal artificial asparagine deamidation and methionine oxidation.
A Comparison of Superficially Porous Particle-based Column Chemistries for Peptide Mapping
This application note compares three different column chemistries from the BIOshell™ line of columns with superficially porous particles to evaluate their performance in peptide mapping.
Simple Digestion Protocol for Proteomics Sample Prep
Rapid trypsin digest kit yields reliable results in less than 2 hours for mass spectrometry analysis.
Improving Glycoform Coverage for Mucin Preparations
Pretreatment with Mucinase StcE increases glycopeptide identification from mucin samples, enhancing sample preparation efficiency for glycopeptide analysis.
Quality Attribute Determination of Monoclonal Antibodies
Workflows for monoclonal antibody adalimumab characterization ensure drug safety and efficacy through critical quality attribute analysis.
Peptide Mapping LC-MS/MS Analysis Workflow for Adalimumab
An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).
Protein Fingerprinting of a Viral Vector, AAV5
Method development for protein fingerprinting of AAV serotype 5
using both intact mass analysis and peptide mapping, to determine critical quality attributes for gene therapy, utilizing three different columns.
Correlation of FcR Affinity Chromatography with Glycan Pattern and ADCC Activity of a Therapeutic Antibody
Fast assessment of antibody-dependent cell-mediated cytotoxicity (ADCC) and glycoform pattern of a therapeutic antibody rituximab by FcγRIIIa affinity chromatography.
Deglycosylation Kits
Find deglycosylation kits for your research. Review the different features of deglycosylation kits for glycoproteins, enzymatic proteins, and native proteins.
Rapid Glycan Prep & Analysis by LC-MS Workflow
PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.
Released N-Glycan Analysis of a Therapeutic Antibody Using BIOshell™ Glycan Column
This application note describes the released N-Glycan analysis of a monoclonal antibody, cetuximab, labeled with procainamide, using a BIOshell™ Glycan HPLC column.
Strategies for Deglycosylating N-Linked Glycans
Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.
Released N-Linked Glycan Analysis Workflow of Adalimumab
A step-by-step protocol for released N-linked glycan analysis of the monoclonal antibody adalimumab, based on UHPLC-FLR-MS and procainamide labeling.