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Showing 1-30 of 51 results for "Enzymatic Protein Deglycosylation Kit"
Deglycosylation Kits
Deglycosylation Kits
Glycoprotein Deglycosylation
Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.
Methods and Matrices for Mass Spectrometry of Glycans
Glycosylation is known to have profound influence on various physiochemical, cellular and biological functions of proteins. Alterations in this modification are known to affect the immune system and have been associated with various pathological states such as cancer, rheumatoid arthritis...
Methods and matrices for mass spectrometry of glycans
Mass Spectrometry of Glycans, method comparison and products
Protein Deglycosylation
Protein Deglycosylation: PNGase F Proteomics Grade, Native Protein Deglycosylation Kit, Enzymatic Protein Deglycosylation Kit, and ProteoProfile Trypsin In-Gel Digest Kit
O-Linked Glycan Strategies
There is no enzyme comparable to PNGase F for removing intact O-linked sugars. Monosaccharides must be sequentially hydrolyzed by a series of exoglycosidases until only the Gal-b(1-3)-GalNAc core remains. O-Glycosidase can then remove the core structure intact with no...
Phosphorylation Analysis Tools
Phosphorylation is an important covalent post-translational modification (PTM) in cell signalling pathways. Protein phosphorylation is the reversible addition of a phosphate group to a protein or small molecule catalysed by protein kinases. Approximately one third of the 30,000 proteins encoded...
Glycan Labeling
Structural modifications of proteins are essential to living cells. When aberrantly regulated they are often the basis of disease. Glycans are responsible for much of the structural variation in biologic systems, and their representation on cell surfaces is commonly called...
GlycoProfile™ ß-Elimination Kit
Our's GlycoProfile Beta-Elimination Kit allows researchers to perform complete glycoproteomics research by preserving both the O-glycans and protein, specifically remove o-glycans, label o-glycans prior to analysis, have confidence in uniformity of procedure. While it is known that O-glycosylation plays...
A validated amino acid analysis assay for accurate quantification of stable isotope labeled protein reference materials: SIL thyroglobulin case study
With the emergence of mass spectrometry for the clinical measurement of proteins in biological matrices, the development of biological reference materials will continue to grow in importance.
A Rapid, Streamlined Workflow for Glycan Sample Preparation and Analysis by LC-MS
The use of PNGase Fast denaturing buffer and enzyme yielded results similar to a conventional 20-hour protocol with overnight digest while reducing workflow time to about 1 hour with a 15-minute digest.
O-Linked Glycan Strategies
While most N-linked oligosaccharides can be removed using PNGase F, a comparable enzyme for removing intact O-linked sugars has not been identified. Monosaccharides must be sequentially hydrolyzed by a series of exoglycosidases until only the Gal-β(1→3)-GalNAc core remains.
Glycan Sequencing Using Exoglycosidases
Glycan Sequencing Using Exoglycosidases
Post Translational Modification
Post-translational modifications such as glycosylation, phosphorylation, and sulfation, to name a few, serve many functions. As a result, the analysis of proteins and their post-translational modifications is particularly important for the study of diseases where multiple genes are known to...
N-Linked Glycans
N-linked glycosylation, modification, and degradation
Sialic Acid
Review article and products for sialic acid synthesis and signaling.
Yeast Cell Lysis Reagents
Our yeast cell lysis reagents are versatile, phosphate-free, and non-denaturing. CelLytic Y Cell Lysis reagent provides an effective method for yeast cell lysis and protein solubilization. The CelLytic Y Yeast Enzymatic Lysis Kit provides a convenient method for...
Impact of GenElute™-E Purification Method on Accuracy of DNA Quantitation and Downstream Enzymatic Processes
Impact of Purification Method on Accuracy of DNA Quantitation and Downstream Enzymatic Processes. Evaluation of the purity of genomic DNA by UV spectrophotometry, gel electrophoresis, and downstream qPCR using GenElute™-E DNA purification kits.
YeastBuster™ Protein Extraction Reagent for Fast, Efficient Extraction of Proteins from Yeast
YeastBuster™ Protein Extraction Reagent for Fast, Efficient Extraction of Proteins from Yeast
ChIP Troubleshooting
Frequently ask questions about ChIP.
TrueGel3D™ Hydrogel for 3D Cell Culture
TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions
Glycosyltransferases
Glycosyltransferases were initially considered to be specific for a single glycosyl donor and acceptor, which led to the one enzyme-one linkage concept. Subsequent observations have refuted the theory of absolute enzymatic specificity by describing the transfer of analogs of some...
True Gel3D™ Hydrogel Troubleshooting Guide
TrueGel3D hydrogels kits for 3D cell culture troubleshooting guide
Downstream Applications for Plasmid DNA and Substrates
This page shows downstream applications for plasmid DNA and substances that may interfere in each.
Bioluminescent Firefly Luciferase Assays
Firefly luciferase is a widely used bioluminescent reporter for studying gene regulation and function. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues.
Glycoprofile™ Labeling Kits
Glycoprofile Labeling Kits for Glycan Analysis designed for efficient labeling of N-linked, O-linked and glycosylphosphatidylinositol (GPI) anchored glycans using your choice of 2-aminobenzamide (2-AB) or 2-aminobenzoic acid (anthranilic acid; 2-AA) 1,2
Glycosyltransferases: Tools for Synthesis and Modification of Glycans
The presence of multiple functional groups and stereocenters in complex carbohydrates makes them challenging targets for the organic chemist.
Duolink® PLA Troubleshooting Guide
Support information including tips and tricks, frequently asked questions, and basic troubleshooting.

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