Showing 1-30 of 199719 results for "enzyme"
Sabrina Ait Braham et al.
Molecules (Basel, Switzerland), 26(4) (2021-03-07)
This paper aims to investigate the effects of some salts (NaCl, (NH4)2SO4 and Na2SO4) at pH 5.0, 7.0 and 9.0 on the stability of 13 different immobilized enzymes: five lipases, three proteases, two glycosidases, and one laccase, penicillin G acylase...
Chan Hee Lee et al.
International journal of biological macromolecules, 175, 341-350 (2021-02-09)
Enzyme immobilization using inorganic materials has been shown to preserve enzyme activity improving and improve their practical applications in biocatalytic process designs. Proper immobilization methods have been used to obtain high recycling and storage stability. In this study, we compared...
Mohamed Amara Camara et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 990, 174-180 (2015-04-17)
In this study, we present an on-line measurement of enzyme activity and inhibition of Glucose-6-phosphate dehydrogenase (G6PDH) enzyme using capillary electrophoresis based immobilized enzyme micro-reactor (CE-based IMER). The IMER was prepared using a two-step protocol based on electrostatic assembly. The...
Ana Cristina Rodrigues et al.
Enzyme and microbial technology, 79-80, 70-77 (2015-09-01)
The hydrolysis/fermentation of wheat straw and the adsorption/desorption/deactivation of cellulases were studied using Cellic(®) CTec2 (Cellic) and Celluclast mixed with Novozyme 188. The distribution of enzymes - cellobiohydrolase I (Cel7A), endoglucanase I (Cel7B) and β-glucosidase - of the two formulations...
Chad Cummings et al.
Biomaterials, 34(30), 7437-7443 (2013-07-16)
Polymer-based protein engineering (PBPE) offers an attractive method to predictably modify and enhance enzyme structure and function. Using polymers that respond to stimuli such as temperature and pH, enzyme activity and stability can be predictably modified without a dependence on...
Mesut Karahan et al.
Artificial cells, nanomedicine, and biotechnology, 42(4), 262-267 (2013-06-28)
L-glutaminase enzyme produced from Hypocrea jecorina pure culture and polyacrylic acid (PAA) in the presence (Cu2+) ions were composed the ternary complex at pH 7. The properties of free and immobilized enzyme were defined. The effect of various factors such...
Anaïs Bénarouche et al.
Biochimie, 102, 145-153 (2014-03-22)
Lipolytic activities of Yarrowia lipolytica LIP2 lipase (YLLIP2), human pancreatic (HPL) and dog gastric (DGL) lipases were first compared using lecithin-stabilized triacylglycerol (TAG) emulsions (Intralipid) at various pH and bile salt concentrations. Like DGL, YLLIP2 was able to hydrolyze TAG...
Brandon W Weber et al.
The Journal of biological chemistry, 288(40), 28514-28523 (2013-08-16)
All known nitrilase superfamily amidase and carbamoylase structures have an additional glutamate that is hydrogen bonded to the catalytic lysine in addition to the Glu, Lys, Cys "catalytic triad." In the amidase from Geobacillus pallidus, mutating this glutamate (Glu-142) to...
Orsolya Erzsébet Szabó et al.
Carbohydrate polymers, 98(2), 1483-1489 (2013-09-24)
A commercial acidic cellulase enzyme complex was chosen in order to gain detailed information about the effect of low-frequency ultrasound (horn at 40 kHz) on the enzyme activity. The performance of the enzyme under sonication was also evaluated in a...
Weiwei Tang et al.
Journal of pharmaceutical and biomedical analysis, 164, 223-230 (2018-11-06)
Angiotensin converting enzyme (ACE), fusing with FLAG tag, was overexpressed in human embryonic kidney 293T cells. This recombinant FLAG-tagged ACE was immobilized on anti-FLAG antibody coated magnetic beads by affinity method in crude cell lysate for the first time. The...
The acetate-activating enzyme of Rhodospirillum rubrum.
Biochimica et biophysica acta, 16(1), 58-65 (1955-01-01)
The acetate activating enzyme of beef heart.
The Journal of biological chemistry, 206(2), 671-676 (1954-02-01)
Mingxiu Guan et al.
Annals of clinical and laboratory science, 47(6), 676-686 (2017-12-22)
M2 type Pyruvate kinase (PKM2) is the key rate-limiting enzyme of glycolysis, and it mainly exists as a dimer in tumor cells. This study aims to establish an enzyme kinetic assay for serum tumor M2 pyruvate kinase (TuM2-PK), and to...
Montasser Hijazi et al.
Biotechnology and bioengineering, 116(2), 272-282 (2018-11-27)
A great limitation for the usability of free enzymes in organic solvents is their insolubility in these media. Some surfactants are capable of solubilizing enzymes in such media, but they are hard to remove. Covalent modification of enzymes with polymers...
Judith A Egan et al.
Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine, 94, 302-305 (2014-10-12)
Methods are presented to tritiate the enzyme inhibitors pargyline and caracemide.
A simple method for the preparation of crystalline potato phosphorylase and Q-enzyme.
H BAUM et al.
Nature, 171(4361), 983-984 (1953-05-30)
Fikret Türkan et al.
Journal of biochemical and molecular toxicology, 33(8), e22340-e22340 (2019-04-12)
Multiple studies have been recorded on the synthesis and design of multi-aim anti-Alzheimer molecules. Using dual butyrylcholinesterase/acetylcholinesterase inhibitor molecules has attracted more interest in the therapy for Alzheimer's disease. In this study, a tannic acid compound showed excellent inhibitory effects...
Lina Liu et al.
Journal of chromatography. A, 1352, 80-86 (2014-06-11)
A novel method using particle-packing technique to fabricate capillary electrophoresis (CE)-based immobilized enzyme reactor (IMER) was accomplished by utilizing perfusive silica single particles as the frits and large-pore beads as the enzyme supports. The fabrication procedure is rapid and simple;...
Wenjin Li et al.
International journal of medical microbiology : IJMM, 304(8), 958-969 (2014-07-16)
Polysaccharide capsules significantly contribute to virulence of invasive pathogens, and inhibition of capsule biosynthesis may offer a valuable strategy for novel anti-infective treatment. We purified and characterized the enzymes CapD and CapE of the Staphylococcus aureus serotype 5 biosynthesis cluster...
Shengyun Huang et al.
Journal of separation science, 43(17), 3565-3572 (2020-07-07)
Capillary electrophoresis is a modern separation technique characterized by many benefits, which qualify it also for enzyme assays and the study of enzyme kinetics during drug development. Homogeneous or heterogeneous approaches can be followed for the enzymatic incubation. In this...
S A Adediran et al.
Archives of biochemistry and biophysics, 614, 65-71 (2017-01-01)
O-Aryloxycarbonyl hydroxamates have previously been shown to covalently inactivate serine/amine amidohydrolases such as class C β-lactamases and a N-terminal hydrolase, the proteasome. We report here reactions between O-aryloxycarbonyl hydroxamates and another N-terminal hydrolase, penicillin acylase. O-Aryloxycarbonyl hydroxamates, as non-symmetric carbonates...
Aem Nuylert et al.
Chembiochem : a European journal of chemical biology, 18(3), 257-265 (2016-12-04)
A hydroxynitrile lyase from the passion fruit Passiflora edulis (PeHNL) was isolated from the leaves and showed high stability in biphasic co-organic solvent systems for cyanohydrin synthesis. Cyanohydrins are important building blocks for the production of fine chemicals and pharmaceuticals....
Zeynep Aydin Sinirlioglu et al.
Bioresource technology, 146, 807-811 (2013-09-03)
A novel type laccase from Shewanella putrefaciens was identified, expressed in Escherichia coli, characterized, prepared in cross-linked enzyme aggregates (CLEA) for industrial applications and investigated of decolorization activity on malchite green dye. Enzyme characterization was investigated by enzyme assay, SDS-PAGE...
Wenhua Lu et al.
Parasitology research, 119(1), 357-364 (2019-12-15)
Aminopeptidase N is an important metalloenzyme from the M1 zinc metallopeptidase family, which is present in numerous apicomplexan parasites, including Plasmodium, Eimeria, and Cryptosporidium. Aminopeptidase N is a potential drug target, and hence, its properties have been widely investigated. In...
Seiichi Sakamoto et al.
Journal of natural medicines, 72(1), 32-42 (2017-11-23)
Immunoassays are antibody-based analytical methods for quantitative/qualitative analysis. Since the principle of immunoassays is based on specific antigen-antibody reaction, the assays have been utilized worldwide for diagnosis, pharmacokinetic studies by drug monitoring, and the quality control of commercially available products....
Jianquan Luo et al.
Bioresource technology, 147, 260-268 (2013-09-03)
A simple enzyme immobilization method accomplished by promoting membrane fouling formation is proposed. The immobilization method is based on adsorption and entrapment of the enzymes in/on the membrane. To evaluate the concept, two membrane orientations, skin layer facing feed (normal...
Dana E Wong et al.
Colloids and surfaces. B, Biointerfaces, 154, 270-278 (2017-03-30)
Immobilized enzymes enable advances in bioprocessing efficiency and bioactive packaging. Enzyme immobilization onto macroscale solid supports is often limited by low protein loading, inadequate access to substrate, and non-ideal orientation to the solid support; immobilization on nanomaterials has improved activity...
Dapeng Peng et al.
Journal of chromatography. A, 1506, 9-17 (2017-05-27)
This study attempts to develop an enzyme-linked immunoassay (ELISA) using a molecularly imprinted polymer (MIP) as an artificial recognition element. The MIP microspheres were prepared using precipitation polymerization with SM
Jan Schejbal et al.
Journal of separation science, 42(5), 1067-1076 (2019-01-22)
Capillary electrophoresis integrated immobilized enzyme reactors are becoming an increasingly popular alternative for enzyme kinetic and inhibition assays thanks to their unique set of features including cost effectiveness, repeated use of the enzyme, minuscule sample consumption, rapid analysis time and...
Gabriela Piccolo Maitan-Alfenas et al.
Bioresource technology, 192, 670-676 (2015-06-22)
Biomass enzymatic hydrolysis depends on the pretreatment methods employed, the composition of initial feedstock and the enzyme cocktail used to release sugars for subsequent fermentation into ethanol. In this study, sugarcane bagasse was pretreated with 1% H2SO4 and 1% NaOH...

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