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Iron Chelate Solution – Plant Tissue Culture Protocol
To prepare 1L of a 10 X iron chelate solution
Protein Interactions
This protocol describes a method for chemical cross-linking of proteins using formaldehyde. With the exception of zero-length cross-linkers, formaldehyde has the shortest cross-linking span (~2-3 Å) of any cross-linking reagent, thus making it an ideal tool for detecting specific protein-protein
Enzymatic Assay of Superoxide Dismutase
Enzymatic Assay of Superoxide Dismutase
Comet Assay
The Comet Assay, also called single cell gel electrophoresis (SCGE), is a sensitive and rapid technique for quantifying and analyzing DNA damage in individual cells.
Biological Buffers
Our extensive portfolio of high-purity biological buffers in various formulation and packaging formats provide superior solution stability and pH control throughout your bioprocess workflow applications.
Enzymatic Assay and ATP Sensitivity Test of Luciferase
Objective: To standardize a procedure to determine the enzymatic activity of Luciferase and/or to determine the ATP detection limit of Luciferase.
Recycling Micro-Assay of β-NAD and β-NADH
Objective: To standardize a procedure for the recycling micro-assay of β-NAD and β-NADH
Cell Dissociation Reagents
Enzymes like trypsin and collagenase are essential for the detachment of adherent cells and solid tissues for subculture and experiments. Recombinant trypsin, solutions containing EDTA, and non-enzymatic solutions provide stabler, gentler options for wider culture applications.
Enzymatic Assay of Cathepsin B
This procedure applies to all products that have a specification for Cathepsin B activity determined by the liberation of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin.
Phycology Media in Plant Tissue Culture
Phycology media can be prepared from concentrated solutions or from powdered salt mixtures. The concentrated solutions are complete, including vitamins, and should be stored frozen.
Analytical Method: Analytical Quality Assurance, Standard for Sulfite
Preparation of a standard solution for Sulfite
Biomolecule Formulation
Specifically developed for high-risk biopharmaceutical applications, our high-quality process chemicals and excipients help you to minimize the regulatory and quality-associated risks of biomolecule formulation.
Cell Dissociation Protocol with Trypsin
Trypsin is frequently used in cell dissociation from adherent surfaces. We offer a wide variety of trypsin solutions to meet your specific cell line requirements, as well as protocols, troubleshooting ideas, and more.
Plant Culture Media
Plant culture media, such as Murashige and Skoog (MS media), Gamborg’s B5, and Phytamax™ Orchid Medium contain essential micronutrients, macronutrients, organics, vitamins, and plant growth regulators for plant culture of cells, organs, and tissues.
Enzymatic Assay of Lipase Type XIII from Pseudomonas sp. Using a Coupled Enzyme System of Glycerol Kinase and Glycerophosphate Oxidase (EC 3.1.1.3)
Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)
Media Development
Media screening, optimization, and nutrient testing can help find the right media and feed for cell lines, while raw material quality dossiers and characterization help to minimize process variability and expedite raw material qualification.