Showing 1-30 of 206 results for "

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Vincent Hyenne et al.
Developmental cell, 48(4), 554-572 (2019-02-13)
Tumor extracellular vesicles (EVs) mediate the communication between tumor and stromal cells mostly to the benefit of tumor progression. Notably, tumor EVs travel in the bloodstream, reach distant organs, and locally modify the microenvironment. However, visualizing these events in vivo still...
Yun-Han Huang et al.
Lab on a chip, 20(18), 3423-3434 (2020-08-14)
In this paper, a cardiac-and-piezoelectric hybrid system is developed for drug screening. The core structure is a polyvinylidene-fluoride piezoelectric membrane that serves as a flexible structure to interact with hiPSC cardiomyocytes and that measures the contraction profile of cardiomyocytes. This...
Kah Yong Tan et al.
Cytotherapy, 17(8), 1152-1165 (2015-07-04)
Mesenchymal stromal cells (MSCs) are being investigated as potential cell therapies for many different indications. Current methods of production rely on traditional monolayer culture on tissue-culture plastic, usually with the use of serum-supplemented growth media. However, the monolayer culturing system...
Hiroyoshi Mori et al.
JACC. Cardiovascular interventions, 10(23), 2375-2387 (2017-11-06)
This study sought to investigate endothelial coverage and barrier protein expression following stent implantation. Biodegradable polymer drug-eluting stents (BP-DES) have been purported to have biological advantages in vessel healing versus durable polymer DES (DP-DES), although clinical trial data suggest equipoise. Biodegradable...
Dominic Cosgrove et al.
The American journal of pathology, 172(3), 761-773 (2008-02-09)
Previous work has shown that integrin alpha1-null Alport mice exhibit attenuated glomerular disease with decreased matrix accumulation and live much longer than strain-matched Alport mice. However, the mechanism underlying this observation is unknown. Here we show that glomerular gelatinase expression...
Anh La et al.
Tissue engineering. Part A, 24(15-16), 1242-1250 (2018-02-17)
Conferring antithrombogenicity to tissue-engineered vascular grafts remains a major challenge, especially for urgent bypass grafting that excludes approaches based on expanding autologous endothelial cells (ECs) that requires weeks of cell culture. Adipose-derived stem cells (ASCs) are available from most patients...
Federica Michielin et al.
Cell reports, 33(9), 108453-108453 (2020-12-03)
The specification of the hepatic identity during human liver development is strictly controlled by extrinsic signals, yet it is still not clear how cells respond to these exogenous signals by activating secretory cascades, which are extremely relevant, especially in 3D...
Sai P Visweshwaran et al.
Bio-protocol, 10(1), e3482-e3482 (2020-01-05)
The ability of cancer cells to migrate through a complex three-dimensional (3D) environment is a hallmark event of cancer metastasis. Therefore, an in vitro migration assay to evaluate cancer cell migration in a 3D setting is valuable to examine cancer...
Anna Gerhartl et al.
Fluids and barriers of the CNS, 17(1), 19-19 (2020-03-07)
The blood-brain barrier (BBB) is altered in several diseases of the central nervous system. For example, the breakdown of the BBB during cerebral ischemia in stroke or traumatic brain injury is a hallmark of the diseases' progression. This functional damage...
Naiyan Wen et al.
International journal of oncology, 55(4), 879-895 (2019-09-06)
Bromodomain and extraterminal domain proteins, especially bromodomain‑containing protein 4 (Brd4), have recently emerged as therapeutic targets for several cancers, although the role and mechanism of Brd4 in glioblastoma multiforme (GBM) are unclear. In this study, we aimed to explore the...
Aldo Leal-Egaña et al.
Molecular biology of the cell, 28(12), 1612-1621 (2017-04-22)
Tumor development progresses through a complex path of biomechanical changes leading first to cell growth and contraction and then cell deadhesion, scattering, and invasion. Tumorigenic factors may act specifically on one of these steps or have a wider spectrum of...
Benjamin Seelbinder et al.
Biophysical journal, 118(11), 2627-2640 (2020-05-15)
Mechanisms of cellular and nuclear mechanosensation are unclear, partially because of a lack of methods that can reveal dynamic processes. Here, we present a new concept for a low-cost, three-dimensionally printed device that enables high-magnification imaging of cells during stretch....
Tests to predict preeclampsia
Chesley's Hypertensive Disorders in Pregnancy, 221-251 (2015)
Shukei Sugita et al.
Scientific reports, 9(1), 3960-3960 (2019-03-10)
Vascular smooth muscle cells (VSMCs) have two distinct phenotypes: contractile and synthetic. The major difference between these phenotypes lies in the magnitude of the contractile force produced by the cell. Although traction force microscopy (TFM) is often used to evaluate...
Mathew E Berginski et al.
PloS one, 6(7), e22025-e22025 (2011-07-23)
Focal adhesions (FAs) are macromolecular complexes that provide a linkage between the cell and its external environment. In a motile cell, focal adhesions change size and position to govern cell migration, through the dynamic processes of assembly and disassembly. To...
Xing Jian et al.
Proceedings of the National Academy of Sciences of the United States of America, 115(20), E4633-E4641 (2018-05-02)
Both type 1 and type 2 diabetes involve a complex interplay between genetic, epigenetic, and environmental factors. Our laboratory has been interested in the physical interactions, in nuclei of human pancreatic β cells, between the insulin (INS) gene and other...
Daniela Chmiest et al.
Nature communications, 7, 13476-13476 (2016-12-06)
Type-I interferons (IFNs) play a key role in the immune defences against viral and bacterial infections, and in cancer immunosurveillance. We have established that clathrin-dependent endocytosis of the type-I interferon (IFN-α/β) receptor (IFNAR) is required for JAK/STAT signalling. Here we...
Daniel Zingg et al.
Cancer cell, 34(1), 69-84 (2018-07-17)
Human melanomas frequently harbor amplifications of EZH2. However, the contribution of EZH2 to melanoma formation has remained elusive. Taking advantage of murine melanoma models, we show that EZH2 drives tumorigenesis from benign BrafV600E- or NrasQ61K-expressing melanocytes by silencing of genes...
Mary Cathleen McKinney et al.
BMC biology, 14(1), 111-111 (2016-12-17)
Collective neural crest cell migration is critical to the form and function of the vertebrate face and neck, distributing bone, cartilage, and nerve cells into peripheral targets that are intimately linked with head vasculature. The vasculature and neural crest structures...
Somadri Ghosh et al.
Human mutation, 38(12), 1731-1739 (2017-09-05)
The SH2 domain containing inositol phosphatase 2 (SHIP2) dephosphorylates PI(3,4,5)P3 to generate PI(3,4)P2, a lipid involved in the control of cell migration and adhesion. The INPPL1 gene that encodes SHIP2 has been found to be mutated in several cases of...
Desiree M Baron et al.
Human molecular genetics, 28(13), 2143-2160 (2019-02-27)
Aberrant translational repression is a feature of multiple neurodegenerative diseases. The association between disease-linked proteins and stress granules further implicates impaired stress responses in neurodegeneration. However, our knowledge of the proteins that evade translational repression is incomplete. It is also...
Dimitris Missirlis et al.
Scientific reports, 6, 23258-23258 (2016-03-19)
The interplay between specific integrin-mediated matrix adhesion and directional persistence in cell migration is not well understood. Here, we characterized fibroblast adhesion and migration on the extracellular matrix glycoproteins fibronectin and vitronectin, focusing on the role of α5β1 and αvβ3...
Rezvan Mobasseri et al.
Biochemical and biophysical research communications, 483(1), 312-317 (2016-12-28)
Surface modification of the substrate as a component of in vitro cell culture and tissue engineering, using bio-active molecules including extracellular matrix (ECM) proteins or peptides derived ECM proteins can modulate the surface properties and thereby induce the desired signaling pathways...
Mirza Muhammad Fahd Qadir et al.
Nature communications, 11(1), 3265-3265 (2020-07-01)
The culture of live pancreatic tissue slices is a powerful tool for the interrogation of physiology and pathology in an in vitro setting that retains near-intact cytoarchitecture. However, current culture conditions for human pancreatic slices (HPSs) have only been tested...
Bibhas Roy et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(19), 10131-10141 (2020-05-01)
Over the course of the aging process, fibroblasts lose contractility, leading to reduced connective-tissue stiffness. A promising therapeutic avenue for functional rejuvenation of connective tissue is reprogrammed fibroblast replacement, although major hurdles still remain. Toward this, we recently demonstrated that...
Characterization of colony-forming cells in adult human articular cartilage.
Ozbey O, Sahin Z, Acar N, et al.
Acta Histochemica, 116(5), 763-770 (2014)
Armando Lagrutta et al.
Scientific reports, 7, 44820-44820 (2017-03-23)
Severe bradycardia/bradyarrhythmia following coadministration of the HCV-NS5B prodrug sofosbuvir with amiodarone was recently reported. Our previous preclinical in vivo experiments demonstrated that only certain HCV-NS5B prodrugs elicit bradycardia when combined with amiodarone. In this study, we evaluate the impact of...
B A Brown et al.
Journal of cell communication and signaling, 12(3), 529-537 (2017-12-01)
Following interventions to treat atherosclerosis, such as coronary artery bypass graft surgery, restenosis occurs in approximately 40% of patients. Identification of proteins regulating intimal thickening could represent targets to prevent restenosis. Our group previously demonstrated that in a murine model...
CCL2/CCR2 chemokine signaling coordinates survival and motility of breast cancer cells through Smad3 and p42/44MAPK dependent mechanisms
Fang W B, et al.
The Journal of biological chemistry, jbc-M112 (2012)
CCL2/CCR2 chemokine signaling coordinates survival and motility of breast cancer cells through Smad3 and p42/44MAPK dependent mechanisms
Fang W B, et al.
The Journal of Biological Chemistry, jbc-M112 (2012)