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Cloning and Expression

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Restriction Endonucleases - The Molecular Scissors
The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson.
How Transfection Works
This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.
Introduction to Yeast Transformation
Transformation is the process by which exogenous DNA is introduced into a cell, resulting in a heritable change or genetic modification. This was first reported in Streptococcus pneumoniae by Griffith in 1928. Transforming principle of DNA was demonstrated by Avery
Mgat4 May Play a Role in Increased Sialylation by Overexpressing Functional MGAT1 in Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells
MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.
T7 Promoter System
Bacterial Expression Vectors: T7 Promoter System. T7 Vectors for Highest Expression Levels in Bacteria.
Restriction Enzyme Cloning Glossary
Restriction Enzyme Cloning Glossary
SnapFast™ Restriction Site Functions
Learn more about relevant restriction site functions in the SnapFast™ plasmid system. All DNA sections are pre-screened, and where possible modified, to remove any of the restriction sites found within the core SnapFast plasmids to maintain their flexibility.
Troubleshooting in pGEX Expression Vectors
This page describes troubleshooting strategies for cloning the gene or gene fragment into a pGEX expression vector.
Reverse Transfection of Plasmid DNA
Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.
Cloning Genes-of-Interest into a Plasmid Vector
This cloning protocol includes selecting the cloning system and plasmid vector, plasmid restriction digestion, fragment restriction digestion, gel excision, dephosphorylating DNA and more.
Troubleshooting for Molecular Cloning
Molecular cloning is the process of inserting the gene-of-interest (GOI) into a plasmid vector and this vector is then inserted into a cell that expresses the protein encoded by the GOI. Once protein is expressed in the cell, the protein
Competent Cell Selection & E. coli Markers Guide
Select competent cells by application, transformation efficiency, and genotype. We also present a list of E. coli markers for reference.
Synthesis and Biomedical Applications of Polyamino Acids
Synthesis and Biomedical Applications of Polyamino Acids
Transforming E.coli with Engineered Plasmid
Making Competent Cells; Making Agar Plates; Bacterial Transformation; Picking Colonies; Growing Bacteria in Liquid Culture; Freezing Bacteria.
Genotypes, Phenotypes and Markers
A genotype is a list of mutant genes in an organism. In addition to mutations of the genome, other genetic elements such as prophage or plasmids can also be included.
Cloning & Expression Vectors
Expression Vectors for Bacterial, Mammalian and Insect Cell Systems. Featured are a variety of tags, promoters and elements for secretion, transient, stable and bicistronic expression.
3xFLAG® System Expression Vectors for Ultra-Sensitive Detection of Recombinant Proteins
The 3xFLAG system is an improvement upon the original system by fusing 3 tandem FLAG® epitopes for a total of 22 amino acids. Detection of fusion proteins containing 3xFLAG is enhanced up to 200 times more than any other system.
Nucleic Acid Modifying Enzyme Selection Chart (Ultra Pure)
A helpful chart for selection of correct nucleic acid modifying enzyme.
Plasmid Product Nomenclature
The SnapFast system is a versatile plasmid cloning platform that provides a range of functional DNA sequences in an easy to clone format. Hundreds of pre-designed DNA sections that can be easily incorporated into, or transferred between, our range of
Introduction to Cell Transfection
Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques and protocols serve as an analytical tool that facilitates the characterization of genetic functions
Blue-White Screening & Protocols for Colony Selection
Learn about and find protocols for the blue white screen technique used in molecular biology research to identify recombinant bacterial clones for further analysis.
Simplicon™ in vivo Protein Expression System
Simplicon: A non-genome integrating, tunable and sustained protein expression system in human cells, based on a single, polycistronic, and self-replicating synthetic RNA.
Bacterial Transformation Protocols
General protocols for growth of competent cells and their transformation (uptake of DNA).
FLAG® Tag Bacterial and Mammalian Expression Vectors
FLAG® and 3xFLAG® expression vectors and products for bacterial and mammalian expression vectors, detection and purification of proteins.