WST-1 assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for WST-1 reagent preparation and examples of applications. Frequently asked questions and troubleshooting guide for WST-1 assay.
Laccase (EC 184.108.40.206) is a cuproenzyme that oxidizes various types of phenols and similar aromatic compounds aromatic amines with the reduction of molecular oxygen to water, therefore, is used as a biocatalyst.
This procedure is for products with a specification for Trypsin activity using Na-Benzoyl-L-arginine ethyl ester (BAEE) as a substrate. The procedure is a continuous spectrophotometric rate determination (A253, Light path = 1 cm).
Enzymatic methods for food analysis are highly specific and offer considerable time and cost savings over other methods, especially from the sample preparation standpoint. We offer a wide variety of convenient kits and reagents for rapid and reliable enzymatic food