Enzyme activity assays

Applied Filters:

Applications:Enzyme activity assays

Content Type:Protocol

Showing 1-30 of 80 results

Protocol Guide: WST-1 Assay for Cell Proliferation and Viability

WST-1 assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for WST-1 reagent preparation and examples of applications. Frequently asked questions and troubleshooting guide for WST-1 assay.

Enzymatic Assay of Superoxide Dismutase

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

Assay Procedure for Protease

This procedure is for informational purposes including assay Procedure, definition, and calculations for Protease

Pull-down assays

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.

PKB/Akt

We offer many products related to PKB/Akt for your research needs.

Assay Procedure for Glycerol Dehydrogenase

LPL Activity Assay Protocol

Lipoprotein lipase (LPL) hydrolyzes triglycerides associated with VLDL.

Enzymatic Method for Determining Urea (Phenylalanine Assay)

Assay protocol for the colorimetric detection of urea in biological samples using the urea assay kit.

Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride

Enzymatic Assay of Achromopeptidase

Enzymatic Assay of Lysozyme (EC 3.2.1.17)

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

Enzymatic Assay of Chloramphenicol Acetyltransferase

To measure chloramphenicol acetyltransferase activity, this procedure uses DTNB and coenzyme A. The reaction of DTNB with the –SH group on CoA results in a colorimetric increase at 412 nm.

Enzymatic Assay of Alcohol Dehydrogenase (EC 1.1.1.1)

To measure alcohol dehydrogenase activity, this assay uses β-nicotinamide adenine dinucleotide phosphate and a continuous spectrophotometric rate determination at 340 nm.

Alkaline Phosphatase (AP) Protocol

Alkaline phosphatase (AP) is a non-specific phosphomonoester hydrolase that catalyzes the hydrolysis of a wide variety of organic monophosphates.

Assay Procedure for Xanthine Oxidase Microbial

The appearance of uric acid is measured at 293nm by spectrophotometry.

Enzymatic Assay of Lyticase

This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate.

Enzymatic Assay of Hyaluronidase (3.2.1.35)

This procedure may be used for Hyaluronidase products.

Enzymatic Assay of α-Glucosidase by the Modified Boehenger Procedure (EC 3.2.1.20)

Enzymatic Assay of Neuraminidase

Enzymatic Assay of Neuraminidase applies to products that have a specification for neuraminidase content by enzymatic determination.

Enzymatic Assay of Laccase (EC 1.10.3.2)

Laccase (EC 1.10.3.2) is a cuproenzyme that oxidizes various types of phenols and similar aromatic compounds aromatic amines with the reduction of molecular oxygen to water, therefore, is used as a biocatalyst.

Enzymatic Assay of Cellulase

To standardize an enzymatic assay procedure of cellulase.

IFN-γ ELISpot Assays on MultiScreen® IP

The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.

Enzymatic Assay of Trypsin Inhibitor

This technical article described the Enzymatic Assay of Trypsin Inhibitor.

Procedure for Enzymatic Assay of Trypsin (EC 3.4.21.4)

This procedure is for products with a specification for Trypsin activity using Na-Benzoyl-L-arginine ethyl ester (BAEE) as a substrate. The procedure is a continuous spectrophotometric rate determination (A253, Light path = 1 cm).

Enzymatic Food Analysis

Enzymatic methods for food analysis are highly specific and offer considerable time and cost savings over other methods, especially from the sample preparation standpoint. We offer a wide variety of convenient kits and reagents for rapid and reliable enzymatic food

Enzymatic Assay of Pectinase

To measure pectinase activity, a titrimetric stop reaction assay is used. One unit of pectinase will liberate 1 μmol of galacturonic acid from poly-galacturonic acid per hour at pH 4.0 at 25 °C.

Enzymatic Assay of Phytase (EC 3.1.3.8)

Derived from procedure SSPHYT02. Includes template updates to current SOP specifications and incorporation of notes into the procedure.

Enzymatic Assay of Amyloglucosidase (EC 3.2.1.3)

This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.

Enzymatic Method for Determining Glucose-6-Phosphate (Glucose-6-Phosphate Assay)

Assay protocol for the colorimetric detection of glucose-6-phosphate in biological samples using the glucose-6-phosphate assay kit.

Page 1 of 3