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facet applications:Filtration
facet content type:Technical Article
Biotinylation Reagents
Biotin-labelled peptides have many important applications in immunology and histochemistry, such as affinity purification and FRET-based flow cytometry, solid-phase immunoassays, and receptor localization, that exploit the high affinity of streptavidin and avidin for biotin.
Protein Concentration for Large Volumes Samples
For filtering large volumes of biological solutions, we manufacture specialized equipment including centrifugal filtering devices, discs, cells and solvents.
Nanoparticle Ultrafiltration
Filtration methods and membrane filter selection in nanoparticle purification and production.
Filter Validation Studies for Pharmaceutical QC – Factors Affecting Analyte Binding
Syringe membrane filter selection and validation methods to assess analyte loss due to membrane filter adsorption in pharmaceutical quality control (QC) testing.
Virus Concentration by Ultrafiltration
Ultrafiltration using Amicon® Ultra centrifugal devices for concentrating virus in preparation of high titer virus stocks and isolation of virus from samples.
User Guide: Integrity Test Kit for small volume devices
This page describes how to use the Integrity Test Kit for small volume devices to integrity test non-vented and vented filter units with membrane surface areas of less than 15 cm2 (2.3 in2). Larger devices can be integrity tested using
Retentate filtration with Millicup™-FLEX disposable vacuum filtration units
Methods and tutorial video for filtration techniques in contamination analysis using a faster alternative to glass vacuum filtration systems.
Universal Hydrophilic Syringe Filters: Millex® Hydrophilic PTFE
Millex® hydrophilic PTFE syringe filters boast broad chemical compatibility and are HPLC-certified, guaranteeing low extractables. With their robust performance, these filters can be used for a variety of samples without the risk of membrane failure or sample contamination.
Membrane Learning Center
Learn about membranes filters and considerations in membrane selection including chemical compatibility, pore size and MWCO, binding and optical properties, and extractables for protein and small molecule analyte sample preparation, HPLC, LC-MS, and other analyses.
Syringe Filters for Chromatography
Syringe filter selection guide for HPLC, UHPLC, and Ion Chromatography using pore size, filter diameter, chemical compatibility, analyte binding, and extractables.
Rapid Trypsin Digestion of Complex Protein Mixtures for Proteomics Analysis
Reliable and reproducible results in mass spectrometry proteomics analyses are highly dependent on robust sample preparation.
Determination of Stevioside and Rebaudioside A in Stevia leaves, beverages and food additives using HPTLC-MS
HPTLC-MS was used to analyze stevioside and rebaudioside in artificial sweeteners, stevia plants, cola and isotonic drinks with a minimum of sample preparation.
Cannabinoid Analysis of Hemp: Developing an Efficient HPLC Method Workflow
Several different HPLC method options are explored for the efficient and time effective analysis of cannabinoids in a sample of hemp flower. The differences between gradient and isocratic analyses are explored, and the two methods are compared in determining the
Syringe Filters
Answers to common syringe filter selection questions, such as sterile vs non-sterile, how to use, membrane and housing types, and pore size.
Dissolution Testing Filtration
Filtration is used in dissolution testing to end the dissolution process and prepare the sample for HPLC analysis. This page discusses the importance of filtration and utility of hydrophilic PTFE Millex® syringe filters in dissolution testing.