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Showing 1-27 of 27 results
Lentiviral Transduction Protocol
Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.
Integration of Sigma® TransPlex® WTA and the Complete WTA2 Kits with the Illumina Microarray Workflow
Amplification products generated by the TransPlex® WTA and Complete WTA2 kits are suitable for microarray target for expression analyses, and can be incorporated into existing Illumina workflows.
RNAi In Situ
<i>In Situ</i> Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry. This is a protocol describing how to perform in situ hybridization on whole mouse embryos. Here we describe the hybridization procedure, and the localization of the...
MISSION® shRNA Guarantee
Upon purchase of the defined clone set quantity on the shRNA detail page for your gene of interest (this varies, but is typically 5 clones), we guarantee that at least one of those clones for a gene should yield greater...
FACS Titration of Lentivirus Protocol
FACS (Fluorescence-Activated Cell Sorting) provides a method for sorting a mixed population of cells into two or more groups, one cell at a time, based on the specific light scattering and fluorescence of each cell. This method provides fast, objective...
Trophoblast Protocol
This protocol describes a method for culturing TS cell lines. These cells can then be used to study trophoblast differentiation and placental function.
Biodegradable Nanoparticles
Preparation for biodegradable nanoparticles and their use in transfection protocols .
Preparation of Cationic Liposomes & Transfection of Cells - Avanti® Polar Lipids
This procedure describes the Preparation of Cationic Liposomes & Transfection of Cells
X-tremeGENE HP DNA Transfection Reagent Protocol
Cell preparation for transfection Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).
Cancer Research Techniques
By focusing on analysis techniques and protocols specific for Cancer Research, we are putting your research needs first. Protein Tyrosine Kinase and Phosphatase Expression Profiling in Cancer Research.
Antibiotic Selection Optimization Using a Cytotoxicity Profile
A protocol describing how to use a cytotoxicity profile to select antibiotics.
CRISPR Cas 9 Nuclease RNA-guided Genome Editing
Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.
X-tremeGENE™ 9 DNA Transfection Reagent Protocol
Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).
Protocol for Transduction of Human Embryonic Stem Cells (hESCs) using Lentiviral Vectors
Protocol for Transduction of Human Embryonic Stem Cells (hESCs) using Lentiviral Vectors
Protocol for Transducing Mouse Embryonic Fibroblasts (MEF) using MISSION® ExpressMag™ Super Magnetic Kit®
This detailed procedure allows you to transduce Mouse Embryonic Fibroblasts (MEF) using MISSION ExpressMag Super Magnetic Kit.
Genome Editing in Plants with CRISPR/Cas9
Zinc finger nucleases (ZFNs), were introduced by Sigma-Aldrich less than 8 years ago, but in that time the technology of targeted genome editing has advanced rapidly. Most recently, the discovery of the CRISPR/Cas9 pathway has accelerated interest in this field...
MystiCq® MicroRNA® Quantitation System
Method for purification, reverse transcription and quantitative PCR for MicroRNAs using Mysticq reagents
siRNA Protocols
MISSION® siRNA Protocols
Spinoculation Protocol
Complete protocol for Lentiviral transduction of suspension cells. This protocol describes the use of MISSION TRC shRNA Lentiviral Particles for long term silencing and phenotypic observation of suspension cells.
CRISPR Lentiviral Screening: Antibiotic Selection
You are not alone designing successful CRISPR, RNAi, and ORF experiments. Sigma-Aldrich was the first company to commercially offer lentivirus versions of targeted genome modification technologies and has the expertise and commitment to support new generations of scientists.
hESC Transduction Protocol
A detailed protocol for the transduction of human embryonic stem cells using Lentiviral Vectors. Includes product list and references.
X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting
X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting
Lentivirus Protocols
MISSION Protocols in Functional Genomics and RNAi
Preparation of the Lentiviral Transduction Particles Using Packaging Plasmid Mix
Preparation of the Lentiviral Transduction Particles Using Packaging Plasmid Mix
Suspension Stable Cells
General Protocol for Creation of Suspension Stable Cells Using MISSION® Lentiviral Particles (SHVRS)
Escort™ IV Transfection Reagent Protocol
Product manual provides detailed protocol for easy DNA transfection.

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