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Protocol for Transducing Mouse Embryonic Fibroblasts (MEF) using MISSION® ExpressMag™ Super Magnetic Kit®
This detailed procedure allows you to transduce Mouse Embryonic Fibroblasts (MEF) using MISSION ExpressMag Super Magnetic Kit.
CRISPR Cas 9 Nuclease RNA-guided Genome Editing
Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.
Preparation of Cationic Liposomes & Transfection of Cells - Avanti® Polar Lipids
This procedure describes the Preparation of Cationic Liposomes & Transfection of Cells
Lentiviral Transduction Protocol
Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.
X-tremeGENE™ 9 DNA Transfection Reagent Protocol
Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).
X-tremeGENE™ HP DNA Transfection Reagent Protocol
Cell preparation for transfection Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).
Biodegradable Nanoparticles
Preparation for biodegradable nanoparticles and their use in transfection protocols .
Antibiotic Optimization for Cell Transduction
Determine optimal antibiotic concentration for stable cell lines, ensuring efficient selection in transduced cells.
MISSION® shRNA Guarantee
Guarantee efficient gene knockdown with our shRNA clones, ensuring reliable results for targeted transcript suppression.
FACS Titration of Lentivirus Protocol
FACS sorts cells based on light scattering and fluorescence for objective cell analysis.
Preparation of the Lentiviral Transduction Particles Using Packaging Plasmid Mix
Preparation of the Lentiviral Transduction Particles Using Packaging Plasmid Mix
MystiCq® MicroRNA® Quantitation System
Method for purification, reverse transcription and quantitative PCR for MicroRNAs using Mysticq reagents
Escort™ IV Transfection Reagent Protocol
Product manual provides detailed protocol for easy DNA transfection.
X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting
X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting
hESC Transduction Protocol
A detailed protocol for the transduction of human embryonic stem cells using Lentiviral Vectors. Includes product list and references.
CRISPR Lentiviral Screening: Antibiotic Selection
Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.
Genome Editing in Plants with CRISPR/Cas9
ZFNs and CRISPR/Cas9 advance targeted genome editing, opening new research avenues.
Spinoculation Protocol
Protocol for lentiviral transduction of suspension cells using MISSION TRC shRNA Lentiviral Particles for long-term silencing.