Applications
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MISSION® Target ID Library for Human miRNA Target ID and Discovery
MISSION® Target ID Library for Human miRNA Target Identification and Discovery;
Custom Services
We are the exclusive distributor of the TRC shRNA library clones in DNA or lentiviral format and many custom services are available.
RNAi Glossary
A glossary of common RNAi terms including, antisense molecules, functional genomics, gene silencing, knock-down, pseudotype, shRNA and more.
Formats — Glycerol FAQs
Whole-genome TRC shRNA libraries, gene family sets or individual RNAi clones/vectors are available as glycerol stocks. Sigma-Aldrich® answers your frequently asked questions about using shRNA in this format
Pooled Lentiviral shRNA Screens to Identify Essential Cancer Pathways
Presenting an article on Pooled Lentiviral shRNA Screens to Identify Essential Cancer Pathways
miRNA (microRNA) Introduction
Mature microRNAs (miRNAs) are a class of naturally occurring, small non-coding RNA molecules, about 21-25 nucleotides in length. MicroRNAs are partially complementary to one or more messenger RNA (mRNA) molecules, and their main function is to downregulate gene expression...
Experimental Design and Analysis — Experimental Design FAQs
We answer your frequently asked questions about designing shRNA experiments, including appropriate controls, screening techniques, selection and more.
General Adherent Cells
Protocol for creating create stable cell lines from both dividing and non-dividing cells for use with a lentiviral delivery system.
Lentiviral Transduction
In this exercise we will be demonstrating lentiviral transduction on two different cell lines.
MISSION® Lentiviral shRNA
MISSION shRNA reduce the expression of specific target genes by targeting the specific mRNA therefore reducing the corresponding protein expression.
Antisense Oligonucleotides
This article summarizes several of the common mechanisms of antisense gene modulation and more importantly, considerations to take into account when designing an antisense oligonucleotide (ASO).
CRISPR/Cas-GFP Vectors for Rapid Expression Verification and Enrichment of Genome Edited Cells
View experimental data showing crispr/cas expression and enrichment using FACS
Successful Transduction Using Lentivirus
You are not alone designing successful CRISPR, RNAi, and ORF experiments. Sigma-Aldrich was the first company to commercially offer lentivirus versions of targeted genome modification technologies and has the expertise and commitment to support new generations of scientists.
MISSION shRNA Lentiviral Transduction
MISSION® shRNA Lentiviral Transduction application data
Lentivirus Cell Line
Using lentivirus as a means to deliver shRNAs has become standard practice in many labs exploring RNAi.
Delivery of Nucleic Acids Using Polymers
Delivery of Nucleic Acids Using Polymers
Target Identification & Validation for Early Drug Discovery
Target identification, validation and characterization for early stages of drug discovery. Identifying drug target via genetic association, expression profiling.
TRC Design and pLKO.1 Vector FAQs
The MISSION® shRNA clones, designed by the TRC, are pre-cloned into the pLKO.1-Puro vector. This lentiviral vector allows for propagation in bacterial culture and selection of inserts in mammalian cells.
Bioluminescent Firefly Luciferase Assays
Firefly luciferase is a widely used bioluminescent reporter for studying gene regulation and function. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues.
Retinoic Acid and Gene Expression
All-trans retinoic acid (RA, ATRA) is a pleiotropic activation factor that regulates genes associated with normal vertebrate cellular processes such as cell differentiation, cell proliferation, apoptosis, and embryonic development.
esiRNA FAQs (Frequently Asked Questions)
Custom & Predesigned esiRNA FAQ
shRNA FAQs
We answers your frequently asked questions about shRNA, the TRC. MISSION and more.
Oligonucleotide Quality Control & Quality Assurance
The foundation of Sigma's manufacturing process is a robust Quality Management System, which drives compliance to ISO 9001:2008 certification.
A Versatile new Lipofection Reagent for co-transfection of DNA and RNA: X-tremeGENE™ siRNA Transfection Reagent
Small inhibitory RNAs (siRNAs) have become the focus of interest in many laboratories. For the first time, these molecules offer an easy way to knock down the expression of selected genes in mammalian cells without having to resort to classical...
Advanced RNAi Applications — in vivo FAQs
We answer your frequently asked questions about in vivo RNAi applications.
Precision Genetic Modifications
Our scientists’ years of experience and Sigma-Aldrich’s access to multiple advanced gene editing technologies allow us to offer a cell engineering service with unparalleled success. We can engineer a variety of modifications in your cell line of choice to address...
MISSION® shRNA Library: Next Generation RNAi
RNAi technology has been one of the key biological breakthroughs of the last decade and has revolutionized basic biology, gene function studies, and it also holds promise to revolutionize drug discovery and therapeutics. Synthetic siRNAs have delivered the genetic...
MISSION® FAQs
We answer your frequently asked questions about shRNA including available formats, experimental design and analysis, advanced applications, ordering, custom options and much more.
MISSION® siRNA FAQs
We answer your frequently asked questions about siRNA including experimental design and analysis, advanced applications, custom options and much more.

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