Gene expression and silencing

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Successful Transduction Using Lentivirus

Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.

General Adherent Cells

Protocol for creating create stable cell lines from both dividing and non-dividing cells for use with a lentiviral delivery system.

Lentiviral Transduction

In this exercise we will be demonstrating lentiviral transduction on two different cell lines.

MISSION® Application Data

MISSION® Application Data. We are committed to providing you with helpful application notes and data for the MISSION® TRC shRNA libraries.

Custom Services

We are the exclusive distributor of the TRC shRNA library clones in DNA or lentiviral format and many custom services are available.

Pooled Lentiviral shRNA Screens to Identify Essential Cancer Pathways

Presenting an article on Pooled Lentiviral shRNA Screens to Identify Essential Cancer Pathways

Lentivirus Cell Line

Using lentivirus as a means to deliver shRNAs has become standard practice in many labs exploring RNAi.

Delivery of Nucleic Acids Using Polymers

Reverse Transfection of Plasmid DNA

Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.

Oligonucleotide Quality Control & Quality Assurance

The foundation of Sigma's manufacturing process is a robust QMS, which drives compliance to ISO 9001:2008 certification.

Experimental Design and Analysis — Experimental Design FAQs

We answer your frequently asked questions about designing shRNA experiments, including appropriate controls, screening techniques, selection and more.

MISSION® FAQs

We answer your frequently asked questions about shRNA including available formats, experimental design and analysis, advanced applications, ordering, custom options and much more.

esiRNA FAQs (Frequently Asked Questions)

esiRNA are endoribonuclease prepared siRNAs that target the same mRNA sequence for gene silencing. Here are some of the most asked questions regarding esiRNA uses and availability.

General shRNA FAQs

We answers your frequently asked questions about shRNA, the TRC. MISSION and more.

esiRNA Knockdown Efficiency Tested by Western Blotting

Quantitative and qualitative western blotting to validate knockdown by esiRNA.

MISSION^® Lentiviral shRNA

MISSION shRNA reduce the expression of specific target genes by targeting the specific mRNA therefore reducing the corresponding protein expression.

Getting Started with MISSION^® shRNA

RNA interference and siRNAs for gene silencing in molecular biology studies.

X-tremeGENE™ siRNA Transfection Reagent

Small inhibitory RNAs offer easy gene expression knockdown in mammalian cells, revolutionizing gene research.

Bioluminescent Firefly Luciferase Assays

Firefly luciferase is a sensitive reporter for gene studies due to its absence in mammalian cells or tissues.

miRNA (microRNA) Introduction

Mature microRNAs regulate gene expression through various mechanisms; partially complementary to mRNA molecules, downregulating gene expression.

TRC Design and pLKO.1 Vector FAQs

MISSION® shRNA clones in pLKO.1-Puro vector allow bacterial culture propagation and mammalian cell selection for gene silencing studies.

T7 RNA Polymerase - recombinant

T7 RNA Polymerase synthesizes RNA from DNA with high specificity, valuable for in vitro translation and anti-sense RNA.

Antisense Oligonucleotides

This article summarizes several of the common mechanisms of antisense gene modulation and more importantly, considerations to take into account when designing an antisense oligonucleotide (ASO).

MISSION^® shRNA Lentiviral Transduction

Lentiviruses can deliver significant amounts of genetic information into host cells. The resulting lentiviral particles were used to infect the human lung cancer cell line, H1299.

MISSION® siRNA FAQs

We answer your frequently asked questions about siRNA including experimental design and analysis, advanced applications, custom options and much more.

Phosphorothioate Oligonucleotides

Phosphorothioate is a widely used backbone variant effective in creating oligonucleotides for antisense knockdowns.

Using Microarray Technology to Select Housekeeping Genes in Chinese Hamster Ovary Cells

In the present study, we have identifi ed species-specifi c housekeeping genes (HKGs) for Chinese Hamster Ovary (CHO) cells using data from microarray gene expression profiling.

pLKO-1 Vector and shRNA Design Frequently Asked Questions

Frequently asked questions about pLKO-1 vector & shRNA design

CRISPR-based Gene Activation

Sigma has developed a gene activation system based on a fusion of dCas9 to the catalytic histone acetyltransferase (HAT) core domain of the human E1A-associated protein p300.

TRC Library Citations

TRC 1.5 human and mouse shRNA libraries

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