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Target Identification & Validation for Early Drug Discovery
Target identification, validation and characterization for early stages of drug discovery. Identifying drug target via genetic association, expression profiling.
A Versatile new Lipofection Reagent for co-transfection of DNA and RNA: X-tremeGENE™ siRNA Transfection Reagent
Small inhibitory RNAs (siRNAs) have become the focus of interest in many laboratories. For the first time, these molecules offer an easy way to knock down the expression of selected genes in mammalian cells without having to resort to classical
CRISPR/Cas-GFP Vectors for Rapid Expression Verification and Enrichment of Genome Edited Cells
View experimental data showing crispr/cas expression and enrichment using FACS
Successful Transduction Using Lentivirus
Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.
Experimental Design and Analysis — Experimental Design FAQs
We answer your frequently asked questions about designing shRNA experiments, including appropriate controls, screening techniques, selection and more.
MISSION® FAQs
We answer your frequently asked questions about shRNA including available formats, experimental design and analysis, advanced applications, ordering, custom options and much more.
General Adherent Cells
Protocol for creating create stable cell lines from both dividing and non-dividing cells for use with a lentiviral delivery system.
MISSION® siRNA FAQs
We answer your frequently asked questions about siRNA including experimental design and analysis, advanced applications, custom options and much more.
MISSION® shRNA Library: Next Generation RNAi
RNAi technology has been one of the key biological breakthroughs of the last decade and has revolutionized basic biology, gene function studies, and it also holds promise to revolutionize drug discovery and therapeutics. Synthetic siRNAs have delivered the genetic
Expression of the Multiple Sclerosis-Associated MHC Class II Allele HLA-DRB1*1501 Is Regulated by Vitamin D
Expression of the Multiple Sclerosis-Associated MHC Class II Allele HLA-DRB1*1501 Is Regulated by Vitamin D
TRC Design and pLKO.1 Vector FAQs
The MISSION® shRNA clones, designed by the TRC, are pre-cloned into the pLKO.1-Puro vector. This lentiviral vector allows for propagation in bacterial culture and selection of inserts in mammalian cells.
miRNA (microRNA) Introduction
Mature microRNAs (miRNAs) are a class of naturally occurring, small non-coding RNA molecules, about 21-25 nucleotides in length. MicroRNAs are partially complementary to one or more messenger RNA (mRNA) molecules, and their main function is to downregulate gene expression
MISSION shRNA Lentiviral Transduction
MISSION® shRNA Lentiviral Transduction application data
Formats — Glycerol FAQs
Whole-genome TRC shRNA libraries, gene family sets or individual RNAi clones/vectors are available as glycerol stocks. Sigma-Aldrich® answers your frequently asked questions about using shRNA in this format
Advanced RNAi Applications — in vivo FAQs
We answer your frequently asked questions about in vivo RNAi applications.
Lentiviral Transduction
In this exercise we will be demonstrating lentiviral transduction on two different cell lines.
T7 RNA Polymerase - recombinant, expressed in E. coli
T7 RNA Polymerase is a DNA-dependant RNA Polymerase that exhibits a very high specificity for the T7 promoter sequence. The polymerase is useful for synthesizing large amounts of RNA suitable for in vitro translation and anti-sense RNA research.
siRNA FAQ
We answer your frequently asked questions about siRNA including experimental design and analysis, advanced applications, custom options and much more.
MISSION® Application Data
MISSION® Application Data. We are committed to providing you with helpful application notes and data for the MISSION® TRC shRNA libraries.
RNAi Glossary
A glossary of common RNAi terms including, antisense molecules, functional genomics, gene silencing, knock-down, pseudotype, shRNA and more.
Custom Services
We are the exclusive distributor of the TRC shRNA library clones in DNA or lentiviral format and many custom services are available.
Pooled Lentiviral shRNA Screens to Identify Essential Cancer Pathways
Presenting an article on Pooled Lentiviral shRNA Screens to Identify Essential Cancer Pathways
General shRNA FAQs
We answers your frequently asked questions about shRNA, the TRC. MISSION and more.
Retinoic Acid and Gene Expression
All-trans retinoic acid (RA, ATRA) is a pleiotropic activation factor that regulates genes associated with normal vertebrate cellular processes such as cell differentiation, cell proliferation, apoptosis, and embryonic development.
Antisense Oligonucleotides
This article summarizes several of the common mechanisms of antisense gene modulation and more importantly, considerations to take into account when designing an antisense oligonucleotide (ASO).
MISSION® Lentiviral shRNA
MISSION shRNA reduce the expression of specific target genes by targeting the specific mRNA therefore reducing the corresponding protein expression.
Lentivirus Cell Line
Using lentivirus as a means to deliver shRNAs has become standard practice in many labs exploring RNAi.
esiRNA Knockdown Efficiency Tested by Western Blotting
Quantitative and qualitative western blotting to validate knockdown by esiRNA.
Delivery of Nucleic Acids Using Polymers
Delivery of Nucleic Acids Using Polymers
Reverse Transfection of Plasmid DNA
Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.
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