Genomics

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facet applications:Genomics

facet applications:qPCR

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Mgat4 May Play a Role in Increased Sialylation by Overexpressing Functional MGAT1 in Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells

MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.

Choosing the Right Custom qPCR Probe

View a summary of the applications using commonly ordered custom qPCR probes.

Universal ProbeLibrary System Technology

UPL is based on only 165 short hydrolysis probes. They are labeled at the 5' end with fluorescein (FAM) and at the 3' end with a dark quencher dye.

Kapa Biosystems qPCR Reagents and Kits

Quantitative PCR (qPCR) and qRT-PCR reagents and kits that are designed to deliver maximum efficiency and reliable results from a variety of genetic materials.

Quantitative PCR Basics

Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR

PCR Assay Optimization and Validation

PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.

Complete Solutions for PCR Assay Development

Fit-for-use products offer the quality, consistency & documentation necessary for every step of your IVD development and manufacturing process.

Custom Oligos Modifications

We offer over 200 custom DNA oligos modification for both DNA, RNA oligonucleotides and sequencing to probes for gene.

KAPA Express Extract Kit FAQs

Extract DNA from crude samples for PCR applications, including qPCR, using the KAPA Express Extract Kit. See frequently asked questions regarding sample type, optimization and storage.

Minimizing Dimerization of Next-Generation Sequencing Adapters

Turn to our Next-Gen Sequencing Oligos (NGSO) as a potential solution if you are experiencing high levels of adapter dimer formation with custom adapters sourced from other vendors.

RT-PCR Troubleshooting

PCR troubleshooting guide for reliable data in molecular assays.

PCR Technologies Guide

Basic PCR/qPCR/dPCR protocols for assay quality control and specific studies.

Water for PCR Techniques

Learn how water impurities can affect PCR and related methodologies. High purity water purified using ultrafiltration is suitable for PCR and related techniques.

DNA Methylation & Bisulfite Conversion

Bisulfite treatment distinguishes methylated DNA in gene methylation studies, exploiting the stability of methylated cytosine.

Impact of GenElute™-E Purification on DNA Processes

GenElute™-E DNA purification kits ensure accurate DNA quantitation and downstream enzymatic processes.

KiCqStart^® SYBR^® Green Primers FAQs

Primer pairs detect prevalent eukaryotic genes, but may co-amplify other transcripts, emphasizing the importance of comprehensive primer design.

Extract-N-Amp™ Plant PCR Kits

Extract-N-Amp kits extract and amplify genomic DNA, optimized for plant tissue without inhibition concerns.

Cas9-Mediated Recombineering for Kdo2-Lipid A

E. coli CRISPR/Cas-mediated Lambda-Red HR vector system enables gene editing via homology-directed repair.

Locked Nucleic Acid & Minor Groove Binder / Eclipse Dark Quencher

Explore Locked Nucleic Acid and MGB. These oligo modifications offer stability and specificity in molecular analysis, making them ideal for research and diagnostic applications.

Quantitative PCR and Digital PCR Detection Methods

Fluorescent dyes or probes are included in PCR mixes to monitor the change in NA amplicon concentration as the reaction proceeds.

Water for Next-Generation Sequencing

Explore the essential role of water in next-generation sequencing for accurate and efficient genomic analysis

Genomic DNA Preparation Troubleshooting

Description of common problems that can occur during genomic DNA preparation

Polymerase Chain Reaction (PCR) Basics

Polymerase chain reaction (PCR) is a technique that results in exponential amplification of a target DNA sequence.

Troubleshooting PCR and RT-PCR Amplification

This page shows PCR and RT-PCR amplification troubleshooting.

PCR/qPCR Data Analysis

After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.

Colony PCR

Colony PCR reagents and our colony PCR protocol using REDExtract-N-Amp™ PCR ReadyMix™ and JumpStart™ REDTaq® PCR ReadyMix™ reagents.

Oligonucleotide Quality Control by Mass Spectrometry

Mass Spectrometry is the technology of choice for analyzing oligonucleotide synthesis. It enables the most sensitive detection of low levels of by-products, which can affect performance.

Primer and Probe Design

Video: Primer and Probe Design

Quantitative RT-PCR

RT-qPCR products combine the effective of Reverse Transcriptase with hot-start taq-directed antibody in convenient ReadyMixes for probe-based or SYBR® Green based applications.

Direct and Crude Extract qPCR

Rapid qPCR analysis from highly inhibited tissue and blood sample extractions with KAPA PROBE.

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