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Immunohistochemistry

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Prestige Antigens™—Blocking Protocol
Prestige Antigens™ and the corresponding Prestige Polyclonals or Monoclonals are recommended to use according to the standard Prestige Antigen-blocking protocol
Immunohistochemistry Procedure
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
Prestige Antibody® Immunohistochemistry Procedure
Prestige Antibody Immunohistochemistry Procedure protocol using standardized tissue microarray slides for antibody validation.
Conjugating Alkaline Phosphatase to Antibodies and Other Proteins
Protocol for the one-step, glutaraldehyde coupling method to conjugation alkaline phosphatase to antibodies and other proteins.
Tips and Techniques for Troubleshooting Immunohistochemistry (IHC)
Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.
Prestige Antibodies® in Immunofluorescence
Subcellular protein location often correlates with function; nucleus proteins implicated in gene regulation, mitochondria proteins in energy production.