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Large molecule HPLC

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Quantification of Infliximab in Human Serum by LC-MS/MS Using A Full-Length Stable Isotope Labeled Internal Standard
Infliximab, a chimeric monoclonal antibody, is used to treat rheumatoid arthritis, psoriatic arthritis, Crohn’s disease, and other autoimmune diseases.
Absolute Quantification of Serum Proteome
Absolute Quantification (Protein-AQUA™™) is a targeted quantitative proteomics technique that exhibits robust efficacy and is being increasingly utilized for a wide variety of quantitative proteomics studies.
New Certified Reference Materials for α‑Lactalbumin, β‑Lactoglobulin A & B, and Lactoferrin
We have developed Certified Reference Materials (CRMs) of fully intact milk proteins that allow accurate testing of the most critical proteins in infant formula; help labs to develop and test infant formula products with high accuracy, while ensuring legal and
SupraSolv® GC Solvent Specifications
Our GC solvents have been specially developed and tested for ECD, FID and MS detection or for headspace gas chromatography to ensure accurate, reliable and reproducible analytical results.
Large Molecule BioAnalysis
Bioanalysis for large molecules, like Proteins or specific Biotherapeutics, refer to a set of methods, assays, and procedures that enable scientists to analyze specific proteins found in living organisms and the biochemical reactions underlying life processes.
Development of Simple and Rapid Workflows for Quantitation of Infliximab and Adalimumab in Human Serum by LC-MS/MS
Development of Simple and Rapid Workflows for Quantitation of Infliximab and Adalimumab in Human Serum by LC-MS/MS
UHPLC Analysis of Conjugated Estrogens on Titan™ C18
The Titan C18 column provided efficient and rapid resolution of thirteen related estrogenic compounds. Ultra Ultra high purity solvents provided robust operation.
HPLC Products: The Best Choice for Any LC Instrument
Discover our comprehensive portfolio of High-performance liquid chromatography (HPLC) columns and consumables for your various and specialized U/HPLC based applications― 100% compatible with HPLC, UHPLC, or UPLC® systems from any vendor.
Optimising the LC-MS Analysis of Biomolecules
We present an article concerning optimising the LC-MS analysis of biomolecules.
An LC/MS Peptide Standard for Rapid System Suitability Assessment and Retention Time Translation Across LC/MS Platforms
Standards are critical in liquid chromatography/mass spectrometry (LC/MS) based proteomics to ensure optimal and consistent system performance before, during, and after sample analysis.
HPLC Troubleshooting Guide
Identify and correct common HPLC problems to restore column performance. Get help with peak problems (peak splitting, peak tailing, peak fronting, etc.), retention time, purge noise, and loss of resolution with example chromatograms.
Application Note – Peroxidase in Vegetables
Application Note: Peroxidase in vegetables – Visual determination after conversion of a specific substrate.
UHPLC Analysis of Vitamin D2 & D3 Metabolites and Epimers on Supel™ Carbon LC Columns
Ultra high-performance liquid chromatographic (UHPLC) separation of Vitamin D Metabolites― 3-epi-25-hydroxyvitamin D2 and 25-hydroxyvitamin D2, using Supel™ Carbon LC column with baseline separation, excellent peak shape, and sensitivity.
UHPLC Analysis of Ephedrine and Related Compounds on Supel™ Carbon LC Column
Ultra-high performance liquid chromatographic (UHPLC) separation of ephedrine and its three structural analogs based on polar retention effect on graphite (PREG) mechanism using Supel™ Carbon LC column.
HPLC Columns for Antibody and Large Protein Analysis
BIOshell™ IgG 1000 Å C4 columns are highly suited for the reversed phase separation of high molecular weight compounds such as monoclonal antibodies with molecular weight of 150 kDa.
Ascentis® Express UHPLC Columns with 2.0 μm Fused-Core Particles
Ascentis® Express columns with 2.0 μm particles show comparable efficiency at a significantly lower pressure than competitor columns that use smaller particles.
UHPLC Analysis of Paraquat and Diquat on Supel™ Carbon LC
Ultra-high performance liquid chromatographic (UHPLC) separation of polar herbicides, paraquat and diquat using SupelTM Carbon LC column with baseline resolution, excellent peak shape, and good lot-to-lot reproducibility.
LC-MS/MS Analysis of Bile Acids using Ascentis® Express C18
Isobaric separation of bile acids and conjugates by LC-MS/MS on Ascentis® Express C18 column with excellent resolution and linearity.
Ascentis® Express Biphenyl UHPLC and HPLC Columns
The bonded, densely endcapped, dimethyl-biphenyl stationary phase of Ascentis® Express 90 Å Biphenyl, based on fused-core particles, provides a stable, reversed phase packing with enhanced π-π and mild steric interactions due to the two sequential phenyl groups bonded to the
Developing HPLC Methods
We recommend every HPLC method developer have these four columns in their arsenal; Ascentis C18, Ascentis RP-Amide, Ascentis Phenyl and Discovery HS F5.
HPLC Analysis of Xanthines on Purospher® STAR RP-18 endcapped
Xanthine is a purine base found in most human body tissues and fluids as well as in other organisms. Methylated xanthines (methylxanthines), which include caffeine, paraxanthine, theobromine, and theophylline, commonly used for their effects as mild stiµlants and as bronchodilators
Ascentis® Express C8 U/HPLC Columns
When a C18 doesn′t give the desired separation, or your sample contains compounds that are known to be difficult to retain or resolve on a C18, consider changing to an Ascentis® Express C8 column.
UHPLC/MS Analysis of Diazepam and Metabolites on Titan™ C18, Comparison of Flow Rate and Column Length
Separation of Oxazepam; Desmethyldiazepam, analytical standard; Temazepam, analytical standard; Diazepam
Physical Stability of a Gel Filtration Column for Antibody Analysis
The results of this column stability study show that TSKgel G3000SWXL are highly stable; the efficiency and peak symmetry values show minimal variation from the first to the 1,000th injection.
BIOshell™ A400 Protein C4 U/HPLC Columns
BIOshell™ A400 Protein C4 columns contain 3.4 μm particles with 400 Å pores, which are derivatized with dimethylbutyl silane and exhaustively endcapped for optimum protein recovery.
HPLC Analysis of Sugars by HILIC Chromatography using Ascentis® Express OH-5 Column with ELSD Detection
Separation of Dextrose, Pharmaceutical Secondary Standard; Certified Reference Material; Sucrose; Maltitol, Pharmaceutical Secondary Standard; Certified Reference Material
UHPLC/MS Analysis of Spice Cannabinoids on Titan™ C18
Synthetic cannabinoids (e.g. "Spice") are a type of designer drug that provides a cannabis-type high.
LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5 (Monitored Ions)
The metabolic role and health implications of the various vitamin D isoforms are of current clinical interest. Therefore, it is important to have an analytical method that will resolve all of the known isoforms with necessary sensitivity and specificity
HPLC Tips & Tricks – Mobile Phase Preparation
Tips and tricks to prepare the correct and consistent mobile phase for optimized separations during your High-performance Liquid Chromatographic (HPLC) based method developments and optimizations.
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