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Maintenance of Multimodal Chromatography Media and Storage Conditions
This page describes the maintenance of media and storage conditions for multimodal chromatography using Cytiva products.
Immobilized Metal Chelate Affinity Chromatography (IMAC)
This page shows how to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography using HiTrap Chelating HP, Chelating Sepharose Fast Flow,His MicroSpin Purification Module or HisTrap Kit from Cytiva.
Lewatit® Ion Exchange Resins – All-around Solutions for Chemical Processes
Chemical processes comprise the steps of processing chemical starting materials, synthesizing the products, isolating the generated materials from the reaction mixture and subsequent purification.
UHPLC Analysis of Ephedrine and Related Compounds on Supel™ Carbon LC Column
Ultra-high performance liquid chromatographic (UHPLC) separation of ephedrine and its three structural analogs based on polar retention effect on graphite (PREG) mechanism using Supel™ Carbon LC column.
Characteristics of Dextrin Sepharose® High Performance Products
This page shows the characteristics of Dextrin Sepharose High Performance products from Cytiva.
UHPLC Analysis of Vitamin D2 & D3 Metabolites and Epimers on Supel™ Carbon LC Columns
Ultra high-performance liquid chromatographic (UHPLC) separation of Vitamin D Metabolites― 3-epi-25-hydroxyvitamin D2 and 25-hydroxyvitamin D2, using Supel™ Carbon LC column with baseline separation, excellent peak shape, and sensitivity.
LC-MS/MS Analysis of Bile Acids using Ascentis® Express C18
Isobaric separation of bile acids and conjugates by LC-MS/MS on Ascentis® Express C18 column with excellent resolution and linearity.
UHPLC Analysis of Paraquat and Diquat on Supel™ Carbon LC
Ultra-high performance liquid chromatographic (UHPLC) separation of polar herbicides, paraquat and diquat using SupelTM Carbon LC column with baseline resolution, excellent peak shape, and good lot-to-lot reproducibility.
Determination of Ephedrine HCl and Pseudoephedrine HCl in Xiao’er Kechuanling Oral Solution using a Discovery® HS C18 Column
Developing an HPLC method for quantifying ephedrine HCl and pseudoephedrine HCl in traditional Chinese medicine (TCM) with a Discovery® C18 column to report: chromatographic data, linearity, specificity and repeatability, LOD and LOQ.
Troubleshooting Guide for Affinity Chromatography of Tagged Proteins
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
Performing a Purity and Homogeneity Check
This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.
Capto Adhere Anion Exchanger for MAbs & Bind/Elute
Capto adhere from Cytiva is a multimodal strong anion exchanger for BioProcess applications.
Capto MMC
Capto MMC is a multimodal cation exchanger with the properties of a weak cation exchanger. In addition to electrostatic interactions, the ligand structure provides for additional interaction modes such as hydrophobic interaction, hydrogen bonding, and thiophilic interaction.
Desalting and Buffer Exchange for Affinity Chromatography of Antibodies
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
HPLC analysis of Letrozole and its impurities by following the USP Guidelines Using a Purospher® STAR RP18e Column and UV Detection
An application note concerning the HPLC analysis of letrozole and its impurities by following the USP43-NF38 Guidelines using a Purospher® STAR RP18e Column.
QuEChERS Cleanup and Analysis of Veterinary Drugs in Milk by LC/MS/MS
QuEChERS Cleanup and Analysis of Veterinary Drugs in Milk by LC/MS/MS
Non-volatile and Volatile Buffer Systems
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
HPLC Analysis of Fenofibrate and Its Impurities by Following the USP43-NF38 Guidelines Using a Purospher® STAR RP18e Column
An application note concerning the HPLC analysis of fenofibrate and its impurities by following the USP43-NF38 Guidelines using a Purospher® STAR RP18e Column.
Determination of Chlorogenic acid and Baicalin in compounded Lonucerae Japonicae Flos with Purospher® STAR RP-18e
Extraction and analysis of chlorogenic acid and baicalin active ingredients in traditional Chinese medicine (TCM) according to Chinese pharmacopeia monograph by HPLC with Purospher® RP-18e column
HPLC determination of Besifloxacin in Ophthalmic Suspension using Chromolith® SpeedROD RP-18e Column
A new HPLC method is proposed for the determination of Besifloxacin, a fluoroquinolone antibiotic in ophthalmic suspension using a Chromolith® SpeedROD RP-18e column. Read now!
LRA (Lipid Removal Agent) for Biopharmaceutical Production
Lipids are naturally-occurring hydrocarbons that are poorly soluble in water and soluble in non-polar solvents such as ether and chloroform.
Column, Media and Sample Preparation for Hydrophobic Interaction Chomatography
Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with Cytiva media are detailed, including sample application, load volume and temperature
Purification Using Protein A-based Chromatography Media
This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.
Purification or Removal of Viruses including Adeno-associated Virus
This page shows how to purify or remove viruses with a Capto DeVirS, AVB Sepharose High Performance from Cytiva.
Purification using GST SpinTrap™
This page shows how to purify GST-tagged proteins using GST SpinTrap™ from Cytiva.
Polishing of MAbs Using Capto Adhere ImpRes in Bind/Elute Mode
In these studies, the binding capacity for MAbs and the efficiency in the clearance of impurities using Capto adhere ImpRes in bind/elute mode was evaluated.
Purification or Removal of Proteins and Peptides
This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.
Principles and Standard Conditions for Different Purification Techniques
This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.
Affinity Chromatography Troubleshooting
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Determination of Nebivolol and its Related Substances in Bulk Drug with Ascentis® Express Phenyl Hexyl Column
An application note concerning the analysis of Nebivolol and its related substances in bulk drugs by following the Indian Pharmacopoeia method. Read now!
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