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Optimized PCR-based Detection of Mycoplasma
Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.
GenomePlex Whole Genome Amplification Kit Protocol (WGA1)
GenomePlex is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative, 500-fold amplification of genomic DNA
Integration of Sigma® TransPlex® WTA and the Complete WTA2 Kits with the Affymetrix Microarray Workflow
TransPlex WTA amplification product is suitable as microarray target for expression analysis on the Affymetrix platform, and can be incorporated into existing Affymetrix workflows
Standard Reverse Transcription Protocol (Two-step)
reverse transcription is the analysis of gene expression to measure concentration mrna a gene. there are several challenges such analyses, as differences in halflife between different transcripts, temporal patterns and lack correlation protein.
HighPrep™ PCR Clean Up Kit and Purification System Protocol
HighPrep™ PCR purification magnetic clean-up beads for manual and automated post-PCR, post-enzymatic reaction, NGS library clean-up.
TeloTAGGG Telomerase PCR ELISA Protocol & Troubleshooting
The TeloTAGGG Telomerase PCR ELISA is used for sensitive qualitative detection of telomerase activity.
REDExtract-N-Amp™ Seed PCR Kit Protocol
The REDExtract-N-Amp Seed PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from seeds (soybean, corn, wheat, etc.).
ABI3900 Synthesizer Columns with CUTAG CPG Frits
Next generation CPG Frit support for reliable, cost-effective oligo synthesis from Proligo®, for ABI 3900 and MerMade synthesizers
GenomePlex® Single Cell Whole Genome Amplification Kit (WGA4) - Protocol
GenomePlex® is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative amplification of genomic DNA
Oligonucleotide Melting Temperature
Calculate the melting temperature (Tm) of oligonucleotides using theoretical method like the nearest neighbor or the basic method. The DNA Tm is the temperature at which 50% of the oligonucleotide is duplexed with its perfect complement and 50% is free...
REDExtract-N-Amp™ Plant PCR Kit Protocol
The REDExtract-N-Amp Plant PCR kit includes an inert loading dye in the ReadyMix for easy gel loading after amplification. This protocol provides a rapid protocol to extract plant genomic DNA for amplification using the optimized ReadyMix.
Integration of Sigma® TransPlex® WTA and the Complete WTA2 Kits with the NimbleGen™ Expression Microarray Workflow
Amplification products generated by the TransPlex® WTA and Complete WTA2 kits are suitable for microarray target for expression analyses, and can be readily integrated into existing NimbleGen workflows.
AptaTaq Fast DNA Polymerase Protocol & Troubleshooting
AptaTaq Fast DNA Polymerase Protocol & Troubleshooting
Method for PCR amplification of Bacterial DNA with low contamination using MTP™ Taq DNA Polymerase (D7442)
Method for PCR amplification of Bacterial DNA with low contamination using MTP™ Taq DNA Polymerase (D7442)
Amplification of DNA Using Jumpstart™ Taq DNA Polymerase (D9307)
Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
Extraction & Amplification of whole blood using WGA-Protocol
Whole blood is a common source of material used to perform genetic analysis. Many times genomic DNA isolated from whole blood samples is of low yield. This protocol is a simple method to isolate DNA from fresh or aged...
Therma™ PCR Additive
PCR additive for the annealing and extension steps that reduce scatter and increase product signals.
Amplification of DNA Using Jumpstart™ Taq DNA Polymerase (D4184)
Hot Start Taq Polymerase protocol to reduce non-specific amplification, with MgCl2 Optimization
Solubilizing Peptides
Solubilizing Custom Peptides
Digital PCR
Digital PCR is an end-point PCR method that is used for absolute quantification and for analysis of minority sequences against a background of similar majority sequences, e.g., quantification of somatic mutations.
DNase I Recombinant, RNase-free Protocol
DNase I from bovine pancreas is a glycoprotein of Mr 37000. A special procedure is used to remove RNases from the DNase preparation.
REDExtract-N-Amp Plant PCR Kits Protocol
The REDExtract-N-Amp Plant PCR Kits contain all the reagents needed to rapidly extract and amplify genomic DNA from plant leaves
SeqPlex Enhanced DNA Amplification Kit (SEQXE) Protocol
The SeqPlex DNA Amplification Kit for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from extremely small quantities or from degraded/highly fragmented DNA
Amplification of DNA Using Jumpstart™ REDTaq® DNA Polymerase (D8187)
Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
Saliva DNA Extraction & WGA Amplification Protocol
Whole Genome Amplification can be performed on DNA extracted in many ways. We offer many products for DNA extraction, including the GenElute™ Blood Genomic DNA Kit, GenElute Mammalian Genomic DNA Miniprep Kit and the GenElute Plant Genomic DNA M iniprep.
Antibody-Enzyme Mediated Hot Start PCR Protocol
JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.
DNA Amplification using Illustra PuReTaq Ready-To-Go PCR Beads
This page shows DNA amplification using illustra PuReTaq ready-to-go PCR beads from Cytiva.
Long and Accurate PCR Amplification of DNA with RedAccuTaq® (D4812)
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. Red dye allows direct loading of reaction on a gel. REDAccuTaq LA
Amplification of Damaged DNA with Restorase DNA Polymerase (R1028)
Method for amplification of DNA from damaged DNA sources. Particularly useful for DNA extracted from old samples.