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Troubleshooting for Molecular Cloning
Molecular cloning is the process of inserting the gene-of-interest (GOI) into a plasmid vector and this vector is then inserted into a cell that expresses the protein encoded by the GOI. Once protein is expressed in the cell, the protein...
Quantitative PCR and Digital PCR Detection Methods
Fluorescent dyes or probes are included in PCR mixes to monitor the change in NA amplicon concentration as the reaction proceeds.
Using PCR to Detect Viral Agents in Animal Sera
The PCR method for detection of viral agents in FBS and other animal sera can help to ensure virus-free serum for cell culture.
Technical Guide to PCR Technologies
Examples of basic PCR/qPCR/dPCR protocols that can be used as the foundation for explorations into some of the concepts described in the theoretical chapters of this guide. Included are detailed protocols for assay quality control, in addition to more general...
PCR Assay Optimization and Validation
PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.
Whole Genome Amplification for Single Cell Biology
Whole genome amplification (WGA) offers a means to overcome the above restrictions for single-cell genomic analyses. WGA has been described as a non-specific amplification technique that affords an amplified product completely representative of the initial starting material.
Optimizing Crude Sample Plant PCR
Overcoming potent inhibitors and sample diversity in direct plant PCR.
PCR Troubleshooting Tips
When developing a PCR troubleshooting protocol, it is important to be open to any possible sources of error, however insignificant they may seem, in order to explore each potential problem independently.
FastStart™ Taq DNA Polymerase, dNTPack Protocol & Troubleshooting
The choice of the PCR enzyme in combination with an appropriate buffer can profoundly affect PCR outcome.
Working with PCR
General Considerations; Factors to Consider in RT-PCR; Prevention of Carryover Contamination; Troubleshooting
Complete Solutions for PCR Assay Development
Fit-for-use products offer the quality, consistency & documentation necessary for every step of your IVD development and manufacturing process.
Long and Accurate PCR
Long and accurate PCR applications address the needs for longer read lengths, greater fidelity and higher yields than that which can be achieved with Taq DNA polymerase.
GC-RICH PCR System Troubleshooting
GC-RICH Amplification of Polymerase Chain Reaction (PCR) System Troubleshooting.
ThermaGenix Product Overview
How to stop nonspecific bands and off target primers in PCR.
Introduction and Historical Timelines
Learn about the history of the polymerase chain reaction (PCR), from the basic principles that proceeded its discovery to the awarding of a Nobel Prize for Chemistry and more recent developments such as real-time PCR (qPCR) and digital PCR.
Locked Nucleic Acid
Frequently asked questions about Locked Nucleic Acids (LNA)
ThermaStop™ PCR Additive
PCR additive added to master mix that stops nonspecific bands after cool down.
Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA)
Preserved samples from medical, forensic, museum and other archival collections represent a rich source of study material, much of it meticulously collected, characterized and preserved through many decades of work by experts in the field.
FastStart™ Taq DNA Polymerase, 5 U/μL Protocol & Troubleshooting
The choice of the PCR enzyme in combination with an appropriate buffer can profoundly affect PCR outcome.
RT-PCR / RT-qPCR Troubleshooting
Developing a PCR or RT-PCR/RT-qPCR troubleshooting protocol so that data are reliable is essential. Potential sources of RT-PCR or PCR error and problems include operator error, the PCR master mix, and oligo design. This PCR troubleshooting guide outlines and details...
Extract-N-Amp™ Plant Tissue PCR Kits
The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that...
Retinoic Acid and Gene Expression
All-trans retinoic acid (RA, ATRA) is a pleiotropic activation factor that regulates genes associated with normal vertebrate cellular processes such as cell differentiation, cell proliferation, apoptosis, and embryonic development.
Oligonucleotide Quality Control & Quality Assurance
The foundation of Sigma's manufacturing process is a robust Quality Management System, which drives compliance to ISO 9001:2008 certification.
Mycoplasma PCR ELISA Protocol
Mycoplasmas are potential contaminants in mammalian cell culture manufacturing. All products produced in cell culture to be tested for the presence of Mycoplasma
Precision Genetic Modifications
Our scientists’ years of experience and Sigma-Aldrich’s access to multiple advanced gene editing technologies allow us to offer a cell engineering service with unparalleled success. We can engineer a variety of modifications in your cell line of choice to address...
Extract-N-Amp™ Blood PCR Kit Protocol
The Extract-N-Amp Blood PCR Kits contain all the reagents needed to rapidly extract and amplify human genomic DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.
PCR Master Mix
A PCR master mix is a batch of PCR or RT-PCR reagents that can be divided among many PCR reaction tubes. It usually includes DNA polymerase, dNTPs, MgCl2 and buffer. Make your own master mix or choose a commercial one.
KAPA Express Extract Kit FAQs
Frequently asked questions (FAQs) for KAPA Express Extract Kit.
KAPA2G Robust PCR Kits FAQs
Frequently asked questions (FAQs) for KAPA2G Robust PCR Kits.
A highly efficient method to concentrate DNA for forensic STR genotyping using DNAstable®
Forensic laboratories routinely use STR genotyping for identity testing of biological samples. However, forensic samples often contain low copy numbers of target DNA, making it difficult to obtain complete STR profiles.